Gene chip for detecting several kinds of common pathogenic bacteria and its prepn process and kit

A gene chip and pathogen technology, applied in the field of bioengineering technology and medical diagnosis, can solve the problems of time-consuming and laborious, single detection method, etc., and achieve the effect of easy use
CN101045945AInactive Publication Date: 2007-10-03IPE BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
IPE BIOTECHNOLOGY CO LTD
Publication Date
2007-10-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.
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Description

technical field

[0001] The invention relates to the fields of bioengineering technology and medical diagnosis, in particular to a gene chip of nine common bacterial pathogens, a preparation method of the chip and a detection kit. Background technique

[0002] Nine kinds of common bacterial pathogens described in the present invention are as follows:

[0003] Staphylococcus aureus

[0004] Staphylococcus epidermidis

[0005] Pseudomonas aeruginosa

[0006] Enterococcus faecium

[0007] Enterococcus faecalis

[0008] Escherichia coli uidA 211bp (Escherichia Coli)

[0009] Enterobacter cloacae ECL

[0010] Klebsiella pneumoniae Hemolysin gene (Klebsiella pneumoniae)

[0011] Acinetobacter baumannii

[0012] The existence of bacterial pathogens seriously threatens human health. The invention detects a variety of common bacterial pathogens, and has more clinical significance especially for the detection of burn infection. Because of the large amount of necrotic and dege...

Claims

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