Gene chip for detecting several kinds of common pathogenic bacteria and its prepn process and kit

A gene chip and pathogen technology, applied in the field of bioengineering technology and medical diagnosis, can solve the problems of time-consuming and laborious, single detection method, etc., and achieve the effect of easy use

Inactive Publication Date: 2007-10-03
IPE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection method of the present invention is easy to operate, overcomes the single and time-consuming and labor-intensive problems of the existing detection methods, can effectively and quick

Method used

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  • Gene chip for detecting several kinds of common pathogenic bacteria and its prepn process and kit
  • Gene chip for detecting several kinds of common pathogenic bacteria and its prepn process and kit
  • Gene chip for detecting several kinds of common pathogenic bacteria and its prepn process and kit

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preparation example Construction

[0178] 1.2 Preparation of probes

[0179] At present, oligo DNA synthesis generally uses the solid-phase phosphoramidite triester method to synthesize DNA fragments. This method has the advantages of high efficiency and fast coupling, and has been widely used in DNA chemical synthesis. DNA chemical synthesis is different from enzymatic DNA synthesis, which extends from 5' to 3', but starts from the 3' end. Concrete reaction steps are as follows:

[0180] protecting group

[0181] Use trichloroacetic acid (Trichloroacetic Acid, TCA) to remove the protective group DMT (dimethoxytrityl) of the nucleotides linked to CPG (Controlled Pore Glass) to obtain a free 5'-hydroxyl end, for the next condensation reaction.

[0182] activation

[0183] The phosphoramidite-protected nucleotide monomer is mixed with tetrazolium activator and enters the synthesis column to form phosphoramidite tetrazolium active intermediate (its 3'-end has been activated, but the 5'-end is still protected b...

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Abstract

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

Description

technical field [0001] The invention relates to the fields of bioengineering technology and medical diagnosis, in particular to a gene chip of nine common bacterial pathogens, a preparation method of the chip and a detection kit. Background technique [0002] Nine kinds of common bacterial pathogens described in the present invention are as follows: [0003] Staphylococcus aureus [0004] Staphylococcus epidermidis [0005] Pseudomonas aeruginosa [0006] Enterococcus faecium [0007] Enterococcus faecalis [0008] Escherichia coli uidA 211bp (Escherichia Coli) [0009] Enterobacter cloacae ECL [0010] Klebsiella pneumoniae Hemolysin gene (Klebsiella pneumoniae) [0011] Acinetobacter baumannii [0012] The existence of bacterial pathogens seriously threatens human health. The invention detects a variety of common bacterial pathogens, and has more clinical significance especially for the detection of burn infection. Because of the large amount of necrotic and dege...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCY02A50/30
Inventor 姚志建于勇逄建涛马立人甄志成周骋
Owner IPE BIOTECHNOLOGY CO LTD
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