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Test strip for detecting human novel coronavirus IgG antibody, kit and preparation method of test strip

A technology of coronavirus and test strips, which is applied in the field of immunoassay analysis, can solve the problems of poor stability and sensitivity, and achieve the effects of not easy to decompose, good sensitivity, and improved detection specificity

Active Publication Date: 2020-08-14
BIOISLAND LAB +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, when colloidal gold is used in immunoassays, it is affected by environmental pH and electrolytes, and its stability is slightly poor, and colloidal gold is a particle visible to the naked eye, and its sensitivity is poor

Method used

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  • Test strip for detecting human novel coronavirus IgG antibody, kit and preparation method of test strip
  • Test strip for detecting human novel coronavirus IgG antibody, kit and preparation method of test strip
  • Test strip for detecting human novel coronavirus IgG antibody, kit and preparation method of test strip

Examples

Experimental program
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Effect test

Embodiment 1

[0089] The present embodiment provides a test strip for rapid detection of human novel coronavirus IgG antibody. The test strip includes a PVC bottom plate, and the PVC bottom plate is connected with a sample pad, a binding pad, a nitrocellulose membrane (NC membrane) ) and absorbent pads.

[0090] In this embodiment, the components of the various solutions used are shown in Table 1 below, and the % in the table represents the mass fraction;

[0091] Table 1

[0092]

[0093]

[0094] The preparation step of described test strip comprises:

[0095] (1) Preparation of the sample pad: cut the sample pad into a size of 23mm×300mm, soak it in the sample pad treatment solution, take it out after 1 hour, and dry it at room temperature for 16 hours. agent, sealed, and stored at 4°C for later use;

[0096] (2) Preparation of bonding pad: A piece of glass fiber with a size of 200mm×300mm is completely immersed in 50mL of bonding pad treatment solution, and the roller is evenly...

Embodiment 2～5

[0111] Examples 2-5 provide a plurality of test strips with different concentrations of S1 protein and N protein. The difference from Example 1 is that the working concentrations of the detection line S1 protein and N protein in each embodiment are respectively set as:

Embodiment 2

[0112] Example 2: S1 protein 1.50 mg / mL, N protein 0.50 mg / mL;

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Abstract

The invention provides a test strip for detecting a human novel coronavirus IgG antibody, a kit and a preparation method of the test strip. A nitrocellulose membrane of the test strip is provided witha detection line and a quality control line; the detection line is coated with novel coronavirus nucleocapsid protein and S1 protein, and the quality control line is coated with a chicken IgY antibody; the combination pad of the test strip is coated with a quantum dot labeled goat anti-human IgG antibody and a quantum dot labeled goat anti-chicken IgY antibody. According to the test strip provided by the invention, the sensitivity and specificity of human novel coronavirus IgG antibody detection can be improved, misdiagnosis caused by a false positive result is prevented, and when the test strip is used for repeatedly detecting the same sample, the obtained T / C value is relatively close; and meanwhile, the test strip is simple in use method and can realize on-site rapid detection.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis, in particular to an immunochromatographic detection test strip, a test kit and a preparation method thereof, in particular to a test strip, a test kit and a preparation method thereof for detecting human novel coronavirus IgG antibodies . Background technique [0002] The clinical manifestations of patients with novel coronavirus pneumonia (CoronaVirus Disease 2019, COVID-19) caused by the novel coronavirus (2019-nCoV or SARS-CoV-2): fever, fatigue, dry cough as the main manifestations, nasal congestion, runny nose, etc. Upper respiratory tract symptoms are rare, and hypoxic and hypoxic states will occur. About half of the patients have dyspnea after more than a week, and severe cases rapidly progress to acute respiratory distress syndrome, septic shock, metabolic acidosis that is difficult to correct, and coagulation dysfunction, etc. [0003] The detection of COVID-19 is...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558G01N33/531G01N33/533
CPCG01N33/6854G01N33/582G01N33/587G01N33/588G01N33/56983G01N33/558G01N33/531G01N33/533G01N2333/165G01N2469/20Y02A50/30
Inventor 张志栋解巧丽齐凯歌陈志龙
Owner BIOISLAND LAB
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