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Detection kit for detecting coronavirus antibody and preparation method thereof

A technology for detecting kits and coronaviruses, which is applied in the field of biomedicine and can solve problems such as low efficiency and decreased sensitivity

Pending Publication Date: 2020-10-23
SUZHOU KANGHESHUN MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficiency of this reaction is very low, resulting in a more obvious change in turbidity when there is a higher concentration of antibody in the sample, resulting in a decrease in detection sensitivity

Method used

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  • Detection kit for detecting coronavirus antibody and preparation method thereof
  • Detection kit for detecting coronavirus antibody and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The detection kit for detecting novel coronavirus antibody in this embodiment includes a first reagent, a second reagent and a calibrator, and the volume ratio of the first reagent and the second reagent in the detection kit is 4:1.

[0050] 1. First Reagent

[0051] The first reagent is latex microspheres coated with novel coronavirus recombinant protein, arginine (Arg), sodium chloride (NaCl), trehalose, bovine serum albumin (BSA), MOPSO buffer, preservative (PC- 300) and a mixed solution of solvent water.

[0052] The preparation method of the first reagent in this embodiment specifically includes the following steps:

[0053] 1) Prepare a MES solution with a concentration of 25mM, and adjust the pH of the solution to 6.5 with 6mol / L NaOH to obtain the MES buffer;

[0054] 2) Prepare a MOPSO solution with a concentration of 50mM, add Arg, NaCl, trehalose, BSA, PC-300 to the MOPSO solution in sequence, stir and dissolve, adjust the pH of the solution to 7.0 with 6mo...

Embodiment 2

[0080] The detection kit for detecting novel coronavirus antibody in this embodiment includes a first reagent, a second reagent and a calibrator, and the volume ratio of the first reagent and the second reagent in the detection kit is 5:1.

[0081] 1. First Reagent

[0082] The first reagent is latex microspheres coated with novel coronavirus recombinant protein, arginine (Arg), sodium chloride (NaCl), trehalose, bovine serum albumin (BSA), MOPSO buffer, preservative (PC- 300) and a mixed solution of solvent water.

[0083] The preparation method of the first reagent in this embodiment specifically includes the following steps:

[0084] 1) Prepare a MES solution with a concentration of 25mM, and adjust the pH of the solution to 6.5 with 6mol / L NaOH to obtain the MES buffer;

[0085] 2) Prepare a MOPSO solution with a concentration of 50 mM, add Arg, NaCl, trehalose, BSA, PC-300 to the solution in sequence, stir and dissolve, adjust the pH of the solution to 7.0 with 6mol / L H...

Embodiment 3

[0106] The detection kit for detecting novel coronavirus antibody in this embodiment includes a first reagent, a second reagent and a calibrator, and the volume ratio of the first reagent and the second reagent in the detection kit is 5:1.

[0107] 1. First Reagent

[0108] The first reagent and its preparation method in this example are the same as in Example 2.

[0109] 2. Second reagent

[0110] The second reagent is latex microspheres coated with anti-new coronavirus recombinant protein antibody, arginine (Arg), sodium chloride (NaCl), trehalose, bovine serum albumin (BSA), MOPSO buffer solution, preservative ( PC-300) and a mixed solution of solvent water.

[0111] The preparation method of the second reagent in this embodiment specifically includes the following steps:

[0112] 1) Prepare a MES solution with a concentration of 25mM, and adjust the pH of the solution to 6.5 with 6mol / L NaOH to obtain the MES buffer;

[0113] 2) Prepare a MOPSO solution with a concentr...

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Abstract

The invention discloses a detection kit for detecting a coronavirus antibody. The detection kit comprises a first reagent and a second reagent, the first reagent is a solution containing first microspheres, and the first microspheres are latex microspheres coated with coronavirus recombinant protein; and the second reagent is the solution containing second microspheres, and the second microspheresare latex microspheres coated with anti-coronavirus recombinant protein antibodies. According to the detection kit disclosed by the invention, latex microspheres are coated with a novel coronavirus recombinant protein antigen and a paired monoclonal antibody; whether a novel coronavirus antibody exists in a sample to be detected or not is judged through a change of solution absorbance (absorbance) by utilizing an indirect competition method that the latex microspheres coated with recombinant protein antigens are firstly combined with the novel coronavirus antibody in the sample to be detectedand then combined with the latex microspheres coated with anti-recombinant protein monoclonal antibodies. The detection kit for detecting the novel coronavirus antibody is strong in specificity, highin sensitivity and wide in detection range, and reagent cost can be greatly reduced.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a detection kit for detecting coronavirus antibodies and a preparation method of the detection kit. Background technique [0002] The pneumonia caused by the new coronavirus is an acute infectious disease. Its pathogen is a coronavirus that has not been found in humans before, that is, SARS-CoV-2. The disease caused by it is named COVID-19 by the World Health Organization It is mainly transmitted through respiratory droplets and close contact infection, which is highly contagious and the crowd is generally susceptible to infection. [0003] Since the development of the pneumonia epidemic caused by the new coronavirus, it is necessary to quickly and accurately screen samples on a large scale to provide a basis for the clinical diagnosis of people infected with the new coronavirus and to provide quick and timely judgment results for enterprises to resume work and production. I...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/569G01N33/543
CPCG01N33/6854G01N33/577G01N33/56983G01N33/54346G01N2333/165G01N2469/20
Inventor 张正阳刘向晖王明陈胜胜汪春芳
Owner SUZHOU KANGHESHUN MEDICAL TECH
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