Detection kit capable of being used for detecting neutralizing antibody of novel coronavirus

A detection kit and coronavirus technology, applied in the field of novel coronavirus IgG antibody detection kits, can solve the problem of not being able to indicate the existence of neutralizing antibodies, etc.

Active Publication Date: 2020-08-14
中国疾病预防控制中心病毒病预防控制所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The production of neutralizing antibodies indicates that the condition is getting better or even cured, but none of the existing diagnostic kits for detecting virus antibodies can indicate the presence of neutralizing antibodies

Method used

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  • Detection kit capable of being used for detecting neutralizing antibody of novel coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Preparation and Confirmation of Polypeptides and Proteins

[0023] The peptides of SEQ ID NO: 1 and 2, as well as the RBD domain and N protein of the S protein of the new coronavirus SARS-CoV-2 were commissioned to be synthesized by Nanjing GenScript Biotechnology Co., Ltd.

[0024] The amino acid sequence (single-letter symbol of amino acid) of described polypeptide is as follows:

[0025]

Embodiment 2

[0026] Embodiment 2. Preparation of kit (detection device)

[0027] Kit (detection device) 1

[0028] The solution of the polypeptide shown in the above-mentioned SEQ ID NO: 1 and 2, the solution of the RBD domain of the S protein of the new coronavirus SARS-CoV-2, and the solution of the new coronavirus SARS were respectively spotted on the iPDMS membrane solid phase carrier by conventional methods. -The solution of the N protein of CoV-2, another sample positive quality control point and negative quality control point, prepared a kit (detection device) (chip reaction plate in the form of a 48-well plate).

Embodiment 3

[0029] Embodiment 3. detect with kit

[0030] Inspection steps

[0031] 1. Preparation: Before the test, the reagents and test samples should be equilibrated to room temperature; the serum should be diluted 100 times with serum diluent (50 times for whole blood).

[0032] 2. Wet the chip: Take out the chip reaction plate. Wet the chip surface with cleaning solution for 3 minutes, then discard the cleaning solution.

[0033] 3. Add sample: Take 100 μL of the sample to be tested and add it to the reaction well of the chip.

[0034] 4. Sample incubation: put the chip reaction plate into a constant temperature incubator, and incubate at 37°C and 500rpm for 30 minutes.

[0035] 5. Sample cleaning: Discard the samples in the reaction wells of the chip, rinse the reaction wells with washing solution and shake off, repeat 3 times.

[0036] 6. Add enzyme antibody: add 100 μL enzyme-labeled antibody solution to the reaction well of the chip, and the enzyme-labeled antibody is HRP-...

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Abstract

The invention relates to a detection kit capable of being used for detecting a neutralizing antibody of a novel coronavirus. The novel coronavirus SARS-CoV-2 antibody (IgG) detection device provided by the invention comprises a solid carrier, and a combination of specific polypeptide and protein connected to the solid carrier.

Description

[0001] This application claims the priority of Chinese patent application 202010302216.9 (Name of Invention: Novel Coronavirus Antibody Detection Kit Suitable for Screening People Without Obvious Symptoms, Application Date: April 16, 2020). technical field [0002] The present invention mainly relates to an antibody detection kit. Specifically, the present invention relates to a novel coronavirus (SARS-CoV-2) IgG antibody detection kit. Background technique [0003] The 2020 novel coronavirus (SARS-CoV-2) pneumonia has swept the world, and the prevention and control of the epidemic has become a common task for governments and people of all countries. Disease control experts have put forward the prevention and control measures of "early detection, early isolation, and early treatment", and diagnostic kits have become an indispensable tool for epidemic prevention and control. [0004] The diagnostic kits that have been developed so far can be divided into two categories accor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/545
CPCG01N33/6854G01N33/56983G01N33/545G01N2333/165G01N2469/20
Inventor 谭文杰马宏伟黄保英李一婷王慧娟杨兰程虎王文玲
Owner 中国疾病预防控制中心病毒病预防控制所
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