Detection kit capable of being used for detecting neutralizing antibody of novel coronavirus
A detection kit and coronavirus technology, applied in the field of novel coronavirus IgG antibody detection kits, can solve the problem of not being able to indicate the existence of neutralizing antibodies, etc.
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Embodiment 1
[0022] Example 1. Preparation and Confirmation of Polypeptides and Proteins
[0023] The peptides of SEQ ID NO: 1 and 2, as well as the RBD domain and N protein of the S protein of the new coronavirus SARS-CoV-2 were commissioned to be synthesized by Nanjing GenScript Biotechnology Co., Ltd.
[0024] The amino acid sequence (single-letter symbol of amino acid) of described polypeptide is as follows:
[0025]
Embodiment 2
[0026] Embodiment 2. Preparation of kit (detection device)
[0027] Kit (detection device) 1
[0028] The solution of the polypeptide shown in the above-mentioned SEQ ID NO: 1 and 2, the solution of the RBD domain of the S protein of the new coronavirus SARS-CoV-2, and the solution of the new coronavirus SARS were respectively spotted on the iPDMS membrane solid phase carrier by conventional methods. -The solution of the N protein of CoV-2, another sample positive quality control point and negative quality control point, prepared a kit (detection device) (chip reaction plate in the form of a 48-well plate).
Embodiment 3
[0029] Embodiment 3. detect with kit
[0030] Inspection steps
[0031] 1. Preparation: Before the test, the reagents and test samples should be equilibrated to room temperature; the serum should be diluted 100 times with serum diluent (50 times for whole blood).
[0032] 2. Wet the chip: Take out the chip reaction plate. Wet the chip surface with cleaning solution for 3 minutes, then discard the cleaning solution.
[0033] 3. Add sample: Take 100 μL of the sample to be tested and add it to the reaction well of the chip.
[0034] 4. Sample incubation: put the chip reaction plate into a constant temperature incubator, and incubate at 37°C and 500rpm for 30 minutes.
[0035] 5. Sample cleaning: Discard the samples in the reaction wells of the chip, rinse the reaction wells with washing solution and shake off, repeat 3 times.
[0036] 6. Add enzyme antibody: add 100 μL enzyme-labeled antibody solution to the reaction well of the chip, and the enzyme-labeled antibody is HRP-...
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