31 results about "Microplate Well" patented technology

A cell incubation system and methods for using a cell incubation system are described. The incubator system comprises an incubation compartment for receiving a microplate for processing samples. The microplate comprises a plurality of wells, each of the plurality of wells including a collection chamber, and a plurality of fluidic structures coupled to the plurality of wells. The microplate further comprises a plurality of sensors coupled to the plurality of wells.

The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.

A high capacity microarrayer for spotting solution onto slides in an automated microarray dispensing device. A microplate indexing device automatically moves, in sequence, a plurality of microplates to a solution removal area. A dispense head accesses each microplate at the solution removal area to remove solution from the microplate. The dispense head then moves to a slide positioning station to spot slides at the slide positioning station. In a preferred embodiment of the present invention, the microplate indexing station has at least one input stacking chamber for stacking microplates, and at least one output stacking chamber for stacking microplates. A walking beam indexer is disposed between the at least one input stacking chamber and the at least one output stacking chamber. The walking beam indexer is for moving microplates from said at least one input stacking chamber to said at least one output stacking chamber. While at the solution removal area, a lid lifter lifts the lid off each microplate to permit the microplate to be accessed by the dispense head for solution removal. After the solution is removed, the lid lifter replaces the lid. In another preferred embodiment, there is at least one light source capable of illuminating the slides, and at least one camera operating in conjunction with the at least one light source. The at least one camera is capable of acquiring and transmitting slide image data to a computer. The computer is programmed to receive the slide image and analyze it. The computer will then generate post analysis data based on the analysis of the slide image data. The post analysis data is available for improving the spotting of the solution onto the slides. In a preferred embodiment, the slide image data includes information relating to slide alignment, information relating to spot quality, and slide identification information. In a preferred embodiment, the analysis of the information relating to slide alignment enables the computer to make automatic adjustments to the relative positions of the at least one dispense head and the slides to increase the accuracy of the spotting. In a preferred embodiment, the analysis of the information relating to spot quality identifies a spot as pass or fail. An operator is then able to rework the spot. In a preferred embodiment, the analysis of the slide identification information enables the computer to track each slide.

A microplate having a base and wells is disclosed. The base has mark parts at least on one edge thereof. Each mark part is defined by notches cut in the edge of the base. The wells are provided in the base and arranged in rows and columns. The mark parts can be used as indicia for identifying not only the microplate but also the wells so that the microplate and the wells can be easily identified without writing numerals or marks on the edges of the base with a felt pen or the like.

The invention provides a space transcriptome sequencing chip and method. The chip and the method are used for determining one or more single cell sequences in different space positions in tissues. Thechip comprises a micropore plate and coding sequencing microspheres, wherein the micropore plate is provided with the coding sequencing microspheres. The method comprises the steps of coupling with the microspheres using a plurality of random codes, wherein each random code comprises a molecular marker and a position marker barcode; the molecular marker can detect sequence information of single cells of the tissues, and the position markers are used for recording space position information of the single cells of the tissues. Based on the detection chip and the detection method, the single-cell specific transcriptome information of the tissues can be analyzed and detected, and the spatial positions of thousands of single cells in the tissues can be simultaneously detected.

The invention relates to a dual-amplification method of template linear amplification and multi-biotin signal amplification. The method comprises steps that: nucleic acid is separated from a specimen; T7RNA polymerase linear amplification is carried out, such that an RNA product is obtained; the RNA product obtained through linear amplification is captured onto a microtiter plate, and signal amplification is carried out. The method is advantaged in low cost and more reliable data. With the method, the sensitivity and the detection time are similar to those of a PCR method.

The present invention relates to adapters for microplates. The adapters allow sampling from a liquid growth medium in a microplate well after a microorganism has formed a dense layer of biomass on the surface of the growth medium. The adapters also allow easy transfer of a surface-grown microorganism from all wells of a microplate in one go, when inserted before the microorganism form the layer and then removed after incubation and layer-formation, which also enables easy sampling of the growth medium in the

The invention discloses a method for extracting DNA from plant genome and optimizing an amplification system. The method comprises the following steps: taking a plant tissue sample which is 3-5mm longand 2-3 mm wide into a micro-pore plate; adding zirconia balls into the micro-pore plate; adding a DNA extracting solution into the micro-pore plate; and sealing the micro-pore plate, putting the sealed micro-pore plate onto a tissue grinding apparatus to be ground to form sample tissue liquid; taking the sample tissue liquid as a PCR reaction template to perform PCR amplification. The method canbe applied to quick extraction of DNA from various plant genomes, meets research of the plant molecular biology and the genetics, and rapid identification of genetically modified crops.

The invention relates to a method for rapidly determining the titer of bacteriophage of Enterobacter sakazakii, comprising the following steps of: A. preparing a bacteriophage concentration gradient suspension; B. preparing an Enterobacter sakazakii suspension; C. sequentially adding an Enterobacter sakazakii culture solution, the Enterobacter sakazakii suspension in the step B and the acteriophage concentration gradient suspension in the step A in a micropore plate; and D. scanning and determining in real time, scanning and determining a growth curve in a dynamics determining mode in real time under a certain culture temperature and wave length by utilizing a microplate reader. By using the determining method, the titer of the bacteriophage of the Enterobacter sakazakii can be simply, rapidly and efficiently determined.

Methods and systems for venting a well that receives a liquid. The method includes providing a microplate including a well that has a cavity with an open inlet and a closed end. The cavity extends between the open inlet and the closed end. The cavity is defined by a wall surface having a cross-sectional contour that includes at least one continuous section and at least one discontinuity section. The method also includes depositing a liquid into the open inlet of the well. The liquid enters the cavity and flows toward the closed end to at least partially fill the well. The liquid flows along the continuous section of the wall surface and remains separated from the discontinuity section of the wall surface, thereby maintaining a gas exhaust path along a spacing between the liquid and the discontinuity section as the liquid flows toward the closed end.

The invention discloses an influenza A virus subtype parting detection high-flux kit which comprises a micropore plate, a micropore plate cover, a main electromagnetic chuck, a carboxyl magnetic beadand a reverse primer, wherein the micropore plate cover is matched with the micropore plate, the main electromagnetic chuck is detachably arranged at the bottom of the micropore plate, the carboxyl magnetic bead is connected with a magnetic bead connection forward primer, and a 5' end of the reverse primer is provided with a fluorescent mark. The micropore plate comprises a plurality of micropores, annular projections with inner diameters matched with those of the micropores are arranged on the micropore plate cover, the micropore plate and the micropore plate cover can be placed into a PCR (polymerase chain reaction) instrument, and a PCR tube and a PCR frame are replaced. The kit is high in repeatability and sensitivity, good in specificity and convenient and rapid to operate, and RT-PCR(reverse transcription-polymerase chain reaction) results are detected without electrophoresis.

Microplate reader has measuring space, action source, measuring device for biological structure signals of microplate wells, transport support for positioning wells in relation to an optical axis of the device and controller for the action source, the measuring device and the transport support. An incubation apparatus with a frame accommodates a microplate with wells having bottoms for reducing liquid evaporation. The frame has a first opening surrounded by an inner wall for the microplate and an outer wall parallel to the inner wall and connected thereto by an intermediate bottom so that a channel is formed and the intermediate bottom for accommodating a liquid adjusted to the content of the microplate wells. The incubation apparatus has a support surface with a second opening for a microplate and, as a result, at least a portion of the well bottoms is freely accessible by the second opening.