Influenza A virus subtype parting detection high-flux kit

An influenza A virus, high-throughput technology, applied in the field of biotechnology detection, can solve problems such as lack of good detection methods
CN109136406AActive Publication Date: 2019-01-04浙江以和细胞生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
浙江以和细胞生物科技有限公司
Publication Date
2019-01-04

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Abstract

The invention discloses an influenza A virus subtype parting detection high-flux kit which comprises a micropore plate, a micropore plate cover, a main electromagnetic chuck, a carboxyl magnetic beadand a reverse primer, wherein the micropore plate cover is matched with the micropore plate, the main electromagnetic chuck is detachably arranged at the bottom of the micropore plate, the carboxyl magnetic bead is connected with a magnetic bead connection forward primer, and a 5' end of the reverse primer is provided with a fluorescent mark. The micropore plate comprises a plurality of micropores, annular projections with inner diameters matched with those of the micropores are arranged on the micropore plate cover, the micropore plate and the micropore plate cover can be placed into a PCR (polymerase chain reaction) instrument, and a PCR tube and a PCR frame are replaced. The kit is high in repeatability and sensitivity, good in specificity and convenient and rapid to operate, and RT-PCR(reverse transcription-polymerase chain reaction) results are detected without electrophoresis.
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Description

technical field

[0001] The invention belongs to the field of biotechnology detection, in particular to a high-throughput kit for subtyping and detection of influenza A virus. Background technique

[0002] Influenza A virus is a common influenza virus that mutates very easily, so there are many subtypes of influenza A virus. These subtypes are known as "bird flu". Influenza A is highly pathogenic to humans and has caused worldwide pandemics many times. Among the influenza A viruses, the subtypes of avian influenza viruses that can directly infect humans have been found so far: A H1N1, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 and H10N8. Among them, H1, H5, and H7 subtypes are highly pathogenic, and H1N1, H5N1, and H7N9 deserve special attention. Therefore, it is very necessary to detect influenza A virus subtypes H1N1, H5N1, and H7N9. There are currently no good detection methods for these subtypes. Contents of the invention

[0003] The purpose of the present invention...

Claims

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