Method for detecting tetracycline residues in milk and drinking water

A technology of tetracycline and drinking water, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of time-consuming and high cost, and achieve the effects of high reliability of results, wide application range and simple operation

Inactive Publication Date: 2013-06-12
SHANGHAI JIAO TONG UNIV
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  • Abstract
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Problems solved by technology

Therefore, this method is fast, cheap, high sensitivity, good selectivity, easy to operate and does not need to rely on large-scale equipment, and the minimum detection limit is 45.8nM. cheap advantage

Method used

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  • Method for detecting tetracycline residues in milk and drinking water
  • Method for detecting tetracycline residues in milk and drinking water
  • Method for detecting tetracycline residues in milk and drinking water

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Embodiment 1

[0035] like figure 1 As shown, this embodiment includes the following steps:

[0036] Optimizing the concentration of aptamers in the system: add aptamers in the concentration range of 1-20nM to the nano-gold solution, and finally add 0.1365μM PDDA, and draw the difference between the ratio of the light absorption values ​​at 650nm and 520nm measured by a microplate reader Curve, the aptamer concentration corresponding to the peak is 6nM.

[0037] Optimizing the PDDA concentration in the system: After incubating 6nM tetracycline aptamer with 0.2 and 2 μM tetracycline solution for 15 minutes, add 100 μL nano-gold, and finally add PDDA with a concentration range of 0.084-0.158nM for 5 minutes, and use a microplate reader to The measured difference between the ratio of light absorption values ​​at 650nm and 520nm was plotted, and the peak corresponding to the PDDA concentration was 0.1121nM.

[0038] Prepare a tetracycline detection system of known concentration: Take 9 PE tube...

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Abstract

The invention discloses a method for detecting tetracycline residues in milk and drinking water and belongs to the technical field of food safety. The method comprises the following steps of: constructing a detection system with known tetracycline concentration to obtain a light absorption difference standard curve, fitting the curve to obtain a regression equation, mixing milk or drinking water to be detected with an acetic acid solution to obtain a mixed solution, centrifuging and layering the mixed solution to obtain a supernatant liquid, adding a tetracycline aptamer solution, adding a nanogold solution after incubation, finally adding positive ion electrolyte, determining the difference of the light adsorption rates at the 650nm part and the 520nm part of an electrolyte standard solution and a blank control solution by a microplate reader, and calculating the content of the tetracycline in milk and drinking water to be detected under the control of the equation obtained by fitting the standard curve. The method is high in speed, low in cost, high in sensitivity, good in selectivity, and convenient to operate without depending on large-sized instruments or equipment, the lowest detection limit is 45.8nM, the defects that the existing aptamer is time-wasting due to marking and high in cost are overcome and the method has the advantages of high speed, sensitivity and low cost.

Description

technical field [0001] The invention relates to a method in the technical field of food safety, specifically a method for detecting tetracycline concentration in milk and drinking water with a concentration range of 1-5 μM and a minimum detection limit of 45.8 nM by using cationic electrolytes to gather nano-gold particles. Background technique [0002] Tetracyclines are a class of broad-spectrum antibiotics that control bacterial infections and inhibit protein synthesis. In view of its good bacteriostasis and low price, tetracycline is widely used in human and animal treatment, growth promotion and added to livestock and poultry feed. However, excessive use of tetracycline can induce strain resistance and cause high drug residues, which will cause direct or indirect harm to human health. In order to ensure the food safety of consumers, in 1990, the EU stipulated that the maximum residual amount of tetracycline in milk was 225nM; the US FDA stipulated that the maximum resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 周培贺兰罗艳芳智文婷吴远根詹深山
Owner SHANGHAI JIAO TONG UNIV
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