Method for detecting tetracycline residues in milk and drinking water
A technology of tetracycline and drinking water, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of time-consuming and high cost, and achieve the effects of high reliability of results, wide application range and simple operation
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[0035] like figure 1 As shown, this embodiment includes the following steps:
[0036] Optimizing the concentration of aptamers in the system: add aptamers in the concentration range of 1-20nM to the nano-gold solution, and finally add 0.1365μM PDDA, and draw the difference between the ratio of the light absorption values at 650nm and 520nm measured by a microplate reader Curve, the aptamer concentration corresponding to the peak is 6nM.
[0037] Optimizing the PDDA concentration in the system: After incubating 6nM tetracycline aptamer with 0.2 and 2 μM tetracycline solution for 15 minutes, add 100 μL nano-gold, and finally add PDDA with a concentration range of 0.084-0.158nM for 5 minutes, and use a microplate reader to The measured difference between the ratio of light absorption values at 650nm and 520nm was plotted, and the peak corresponding to the PDDA concentration was 0.1121nM.
[0038] Prepare a tetracycline detection system of known concentration: Take 9 PE tube...
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