Human procalcitonin immunodetection kit, and preparation method and application thereof

Abstract

The invention provides a human procalcitonin immunodetection kit, comprising: an enzyme-labeled plate coated by PCT monoclonal multi-antibody; at least two PCT standard reagent bottles, wherein each of the PCT standard reagent bottles is filled with a PCT standard substance of a different concentration; and an enzyme-labeled reagent bottle which is filled with the enzyme-labeled PCT monoclonal multi-antibody. The immunodetection kit provided by the invention has good sensitivity and specificity; the results of the kit highly accord with the results of a reference reagent; the kit can provide accurate and reliable detections, is simple and easily practicable to operate and is rapid and sensitive in detection; a microplate reader used in the invention is simple and popular; and the kit provides an effective tool for detection of human procalcitonin.

Description

technical field [0001] The invention relates to a detection method for human procalcitonin, in particular to a vde immune detection kit, belonging to the biological field. Background technique [0002] Procalcitonin (PCT) is a calcitonin propeptide substance without hormone activity, a glycoprotein with a molecular mass of 13kD, consisting of 116 amino acids, including N-residue, calcitonin, and calcitonin. 1-57 are N-stumps, 60-92 are calcitonin, and 96-116 are calcitonin. Since 1996, PCT has been used as a new type of differential diagnosis tool for the diagnosis of bacterial infection and its secondary complications. In terms of the diagnosis of such diseases as sepsis, septic shock and severe systemic inflammatory reactions, and the follow-up observation of the process, PCT diagnosis It has obvious advantages and high specificity. The sepsis diagnostic criteria of the International Sepsis Conference in 2001 has already used PCT as one of the diagnostic indicators. In ...

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Problems solved by technology

[0006] (1) Gel method, this method is troublesome and difficult to form automatic detection;

[0007] (2) Enzyme-linked immunosorbent assay, the traditional ELISA method has complex detection operations, long reaction time and low sensitivity;

[0008] (3) Radioimmunoassay, which has a long reaction time, unstable test results, poor repeatability, and radioactive contamination problems;

[0009] (4) Immunoluminescence method, which has strong specificity and high sensitivity, but requires expensive experimental instruments;

[0010] (5) colloidal gold chromatography, this method has low sensitivity, can only be qualitative but cannot be quantitatively detected by experiment;

This kind of product adopts two-step method in the detection, and the detection speed is slow, which leads to long detection time, and the detection accuracy is also insufficient; and this kind of method usually uses PCT monoclonal antibody or polyclonal antibody, and the detection sensitivity is insufficient. It is often impossible to establish a standard curve with good linearity at lower concentrations, resulting in the inability to detect lower concentrations of PCT test samples

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