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Electrochemiluminescence immunosensor based on CdZnTeS quantum dots as well as preparation method and application of electrochemiluminescence immunosensor

A technology of luminescence immunity and electrochemistry, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, which can solve problems such as difficult to overcome cancer treatment methods

Active Publication Date: 2018-06-29
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of cancer is still a difficult challenge

Method used

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  • Electrochemiluminescence immunosensor based on CdZnTeS quantum dots as well as preparation method and application of electrochemiluminescence immunosensor
  • Electrochemiluminescence immunosensor based on CdZnTeS quantum dots as well as preparation method and application of electrochemiluminescence immunosensor
  • Electrochemiluminescence immunosensor based on CdZnTeS quantum dots as well as preparation method and application of electrochemiluminescence immunosensor

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Experimental program
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Effect test

Embodiment 1

[0045] The preparation method of the electrochemiluminescent immunosensor based on CdZnTeS quantum dots and the detection of different concentrations of AFP and CA125 include the following steps:

[0046] (1) Preparation of CdZnTeS quantum dots:

[0047] (a) 0.6g CdCl 2 2.5H 2 O and 0.8 g of L-cysteine ​​were dissolved in 100 mL of ultrapure water and the pH was adjusted to 10 with NaOH to prepare the Cd precursor solution;

[0048] (b) Add 0.1g tellurium powder to 25mL ultrapure water, pass N 2 10min, quickly add 0.08g NaHB 4 , and reflux at 80°C for 30 minutes to prepare a NaHTe precursor solution;

[0049] (c) 0.08g of ZnCl 2 and 0.144g of L-cysteine ​​were dissolved in 30mL of deionized water to prepare the ZnS precursor solution;

[0050] (d) 15 mL of freshly prepared NaHTe precursor solution was added to N2-saturated Cd precursor solution followed by ZnS precursor solution. The obtained mixed solution was heated to about 100° C. to obtain a CdZnTeS quantum dot so...

Embodiment 2

[0072] The preparation method of the visualized electrochemiluminescence immunosensor based on CdZnTeS quantum dots and the detection of different concentrations of AFP and CA125 include the following steps:

[0073] (1) Preparation of CdZnTeS quantum dots:

[0074] (a) 0.8g CdCl 2 2.5 H 2 O and 0.8g L-cysteine ​​were dissolved in 100mL ultrapure water and the pH was adjusted to 10.5 with NaOH to prepare the Cd precursor solution;

[0075] (b) Add 0.12g tellurium powder to 30mL ultrapure water, pass N 2 20min, quickly add 0.12g NaHB 4 , and reflux at 80°C for 30 minutes to prepare a NaHTe precursor solution;

[0076] (c) 0.15g of ZnCl 2 and 0.2g of L-cysteine ​​were dissolved in 30mL of deionized water to prepare the ZnS precursor solution;

[0077](d) Add 18 mL of fresh NaHTe solution to N 2 The saturated Cd precursor solution is then added with the ZnS precursor solution, and the resulting solution mixture is heated to about 110° C. to obtain a CdZnTeS quantum dot sol...

Embodiment 3

[0093] The preparation method of the electrochemiluminescent immunosensor based on CdZnTeS quantum dots and the comparison of the detection results of AFP and CA125 with the ELISA results include the following steps:

[0094] (1) Preparation of CdZnTeS quantum dots:

[0095] (a) 0.75g CdCl 2 2.5H 2 O and 0.8 g of L-cysteine ​​were dissolved in 110 mL of ultrapure water and the pH was adjusted to 10 with NaOH to prepare the Cd precursor solution;

[0096] (b) Add 0.1g tellurium powder to 25mL ultrapure water, pass N 2 10min, quickly add 0.1g NaHB 4 , and reflux at 80°C for 30 minutes to prepare a NaHTe precursor solution;

[0097] (c) 0.12g of ZnCl 2 and 0.18g of L-cysteine ​​were dissolved in 35mL of deionized water to prepare the ZnS precursor solution;

[0098] (d) Add 20 mL of fresh NaHTe solution to N 2 Add the ZnS precursor solution to the saturated Cd precursor solution, and heat the obtained mixed solution to about 110° C. to obtain a CdZnTeS quantum dot solutio...

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Abstract

The invention discloses an electrochemiluminescence immunosensor based on CdZnTeS quantum dots. The electrochemiluminescence immunosensor comprises CdZnTeS-Ab2, Fe3O4@SiO2-Ab1 with closed non-specificbinding sites, and antigens respectively connected with the CdZnTeS-Ab2 and the Fe3O4@SiO2-Ab1 by mutual action between antigens and antibodies; the CdZnTeS-Ab2 is secondary antibodies marked by CdZnTeS quantum dots; the Fe3O4@SiO2-Ab1 is Fe3O4@SiO2 with the surface being modified with primary antibodies. The invention also provides a preparation method and application of the electrochemiluminescence immunosensor. The electrochemiluminescence immunosensor, the preparation method and the application disclosed by the invention have the beneficial effects that the electrochemiluminescence immunosensor is established on the basis of mutual action between the antigens and the antibodies, so that the specificity of detection is greatly improved, the detection speed is fast, the sensitivity is high, the specificity is good, the detection limit is low, the detectable linear range is wide, the operation is simple and visual detection can be realized.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence analysis, in particular to an electrochemiluminescence immunosensor based on CdZnTeS quantum dots and a preparation method and application thereof. Background technique [0002] Cancer is considered the second leading threat to human death. At present, the treatment of cancer is still a challenge that is difficult to overcome. Biomarkers are considered to be an indicator signal of cancer occurrence in the human body. The detection of biomarkers can provide valuable information for early cancer diagnosis and effective monitoring of treatment effects, significantly improving the performance of conventional cancer diagnosis. So far, a variety of immunoassay methods based on the interaction between antigen and antibody have been used for the detection of biomarkers, such as colorimetric immunoassay, fluorescence immunoassay, electrochemical immunoassay, electrochemiluminescence immunoassay, et...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N27/48G01N21/76
CPCG01N21/76G01N27/48G01N33/53
Inventor 丁收年梁秀丽韩亭亭朱红允武锡锦左家莹刘金霞闫其报温雪飞
Owner SOUTHEAST UNIV
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