Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique
An organophosphorus pesticide and ratio fluorescence technology, applied in the field of agrochemical analysis, can solve the problems of inability to distinguish between organophosphorus and carbamate pesticides, expensive instruments, difficult on-site detection, etc. Simple operation, avoid interference effect
Inactive Publication Date: 2013-11-27
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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The invention relates to a method for visualized detection of organophosphorus pesticide residue by a doped quantum dot ratio fluorescence technique. Being a standard curve method, the method includes preparation and quenching of a double-fluorescence emission quantum dot, establishment of a standard curve and detection of organophosphorus pesticide residue. The method is characterized in that: the standard curve is determined under ultraviolet irradiation and is a curve about the corresponding relationship of a fluorescence ratio and a sample concentration, i.e. the standard curve is established by adding sample solutions with gradient concentrations into a quenched doped quantum dot probe dispersion fluid with a concentration of 30-40mcg/ml in order under ultraviolet irradiation, and determining fluorescence ratios successively. For the quenched doped quantum dot, a quenching agent is employed to quench the double-fluorescence of the doped quantum dot probe dispersion fluid to fluorescence intensity that no longer reduces. The method provided in the invention realizes sensitive and high selectivity detection of organophosphorus pesticide residue.
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