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Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique

An organophosphorus pesticide and ratio fluorescence technology, applied in the field of agrochemical analysis, can solve the problems of inability to distinguish between organophosphorus and carbamate pesticides, expensive instruments, difficult on-site detection, etc. Simple operation, avoid interference effect

Inactive Publication Date: 2013-11-27
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

The detection results of these large-scale instruments are accurate and reliable, but they are difficult to use for on-site detection due to the shortcomings of expensive instruments, high detection costs, complex sample pretreatment, and professional training for operators.
In addition, although the "enzyme inhibition rate method + spectrophotometry" has been listed as a national recommended standard (GB / T5009.199-2003), it has become a rapid on-site preliminary screening / qualitative method for organophosphorus and carbamate pesticide residues in fruits and vegetables. One of the mainstream techniques for detection, however, enzyme inhibition methods have a common disadvantage: they cannot distinguish organophosphorus and carbamate pesticides present in the same sample
However, the development of ratiometric fluorescent probes for the detection of organophosphorus pesticide residues has not been reported in the literature so far.

Method used

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  • Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique
  • Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique
  • Method for visualized detection of organophosphorus pesticide residue by doped quantum dot ratio fluorescence technique

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Embodiment 1

[0034] Example 1: Detection of chlorpyrifos and its degradation products

[0035] 1. Preparation of manganese-doped zinc sulfide quantum dots

[0036] First dissolve 50mmol of zinc nitrate in 40mL of deionized water, then take 0.5-7.5mmol of manganese acetate and dissolve it in the above solution to do a series of manganese doping experiments to determine which amount has the highest luminescence yield. Ultrasonic the above mixture for 10 minutes Dissolve completely, then reflux and pass through dry nitrogen for more than 30min to remove oxygen in the solution. Dissolve 50mmol of sodium sulfide in 10mL of deionized water, add dropwise to the above boiling mixture, reflux and vigorously stir for 5h. After cooling, centrifuge, ultrasonically disperse and wash three times with deionized water before redispersing in water. Fluorescence spectrum see figure 1 .

[0037] 2. Quenching of fluorescence with double emission bands of doped quantum dots

[0038] Take 5 mL of manganese...

Embodiment 2

[0043] The detection of embodiment 2 methyl parathion

[0044] 1. Preparation of copper and manganese co-doped zinc sulfide quantum dots

[0045] First dissolve 50mmol of zinc chloride in 40mL of deionized water, then take 0.5-7.5mmol of manganese chloride and copper chloride to dissolve in the above solution and do a series of manganese-involving experiments to determine which amount has the highest luminescence yield. The above mixture was sonicated for 10 minutes to dissolve completely, and then refluxed with dry nitrogen for more than 30 minutes to remove the oxygen in the solution. Dissolve 50mmol of sodium sulfide in 10mL of deionized water, add dropwise to the above boiling mixture, reflux and vigorously stir for 5h. After cooling, centrifuge, ultrasonically disperse and wash three times with deionized water, then redisperse in water (concentration micrograms per milliliter), and set aside.

[0046] 2. Quenching of fluorescence with double emission bands of doped quan...

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Abstract

The invention relates to a method for visualized detection of organophosphorus pesticide residue by a doped quantum dot ratio fluorescence technique. Being a standard curve method, the method includes preparation and quenching of a double-fluorescence emission quantum dot, establishment of a standard curve and detection of organophosphorus pesticide residue. The method is characterized in that: the standard curve is determined under ultraviolet irradiation and is a curve about the corresponding relationship of a fluorescence ratio and a sample concentration, i.e. the standard curve is established by adding sample solutions with gradient concentrations into a quenched doped quantum dot probe dispersion fluid with a concentration of 30-40mcg / ml in order under ultraviolet irradiation, and determining fluorescence ratios successively. For the quenched doped quantum dot, a quenching agent is employed to quench the double-fluorescence of the doped quantum dot probe dispersion fluid to fluorescence intensity that no longer reduces. The method provided in the invention realizes sensitive and high selectivity detection of organophosphorus pesticide residue.

Description

1. Technical field [0001] The invention relates to an analysis method of agricultural chemicals, in particular to a method for visual analysis and detection of trace amount of agricultural chemicals, in particular to a method for visual detection of organophosphorus pesticide residues in doped quantum dot ratio fluorescence mode. 2. Background technology [0002] Doped quantum dots refer to semiconductor nanoparticles formed by introducing transition metal ions or rare earth metal ions into the host. The result of doping often has the property of single excitation and multiple emission bands. As a fluorescent probe with potential application value, compared with traditional organic fluorescent dyes, the fluorescence properties of doped quantum dots have greater advantages: the emission peak is narrow and Symmetry, large Stokes shift, high quantum yield, strong brightness and high photostability. In recent years, the fluorescence properties of doped quantum dots have been wi...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王素华张奎朱后娟马芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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