Preparation method and use method of visual detection chip for multiple pesticide residues based on membrane material
A technology with multiple pesticide residues and detection chips, which is applied in the direction of analytical materials, biological material analysis, and measuring devices, can solve the problems of inability to meet the requirements of on-site rapid measurement, high cost, and difficulty in popularizing and applying daily life, so as to ensure uniformity The effect of stability and reliability, easy operation and low price
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Embodiment 1
[0052] Example 1: Preparation method of multi-residue visualization chip of six kinds of pesticides such as triazophos:
[0053] The solid phase support is a rectangular nitrocellulose membrane (Sartorius CN140, pore size 8 μm), with a size of 0.5 cm (width)×2 cm (length). The sample points on the solid phase carrier include positive control --- positive quality control point, which is goat anti-mouse IgG diluted with 0.01M PBS (pH7.4) to a mass concentration of 2 mg / mL; negative control --- negative quality control point, which is 0.01M PBS (pH7.4); the pesticide hapten-chicken ovalbumin (OVA) conjugates used in the detection point are the following 6 kinds: triazophos-OVA, parathion-OVA, methyl parathion -OVA, carbofuran-OVA, imidacloprid-OVA, chlorpyrifos-OVA), diluted with 0.01M PBS (pH7.4) to a concentration of 5-10mg / mL.
[0054] Spot the above-mentioned positive control, negative control, and diluted 6 kinds of pesticide hapten-chicken ovalbumin (OVA) conjugates on the...
Embodiment 2
[0073] Example 2, Preparation of 8 kinds of pesticide multi-residue visualization chips such as triazophos
[0074]Wherein the solid-phase carrier is a circular nitrocellulose membrane (Sartorius CN140, pore size 8 μm), with a diameter of 1 cm; the sample point on the solid-phase carrier includes a positive control-positive quality control point, which is a goat anti-mouse IgG with 0.01M PBS ( pH7.4) diluted to a mass concentration of 2mg / mL; negative control---negative quality control point, which is 0.01M PBS (pH7.4); the pesticide hapten used in the detection point-chicken ovalbumin (OVA) coupling The substances are the following 8 kinds: triazophos-OVA, parathion-OVA, methyl parathion-OVA, carbofuran-OVA, imidacloprid-OVA, fenpropathrin-OVA, acetamiprid-OVA, Chlorpyrifos-OVA, diluted with 0.01M PBS (pH7.4) to a concentration of 5-10mg / mL.
[0075] Spot the above-mentioned positive control, negative control, and diluted 8 kinds of pesticide haptens-chicken ovalbumin (OVA) ...
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