Preparation method and use method of visual detection chip for multiple pesticide residues based on membrane material

A technology with multiple pesticide residues and detection chips, which is applied in the direction of analytical materials, biological material analysis, and measuring devices, can solve the problems of inability to meet the requirements of on-site rapid measurement, high cost, and difficulty in popularizing and applying daily life, so as to ensure uniformity The effect of stability and reliability, easy operation and low price

Inactive Publication Date: 2014-11-26
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above patent reports all require supporting signal monitoring equipment (biochip scanner, CCD detector, etc.), the cost is relatively high, and it still cannot meet the requirements of on-site rapid detection of pesticide residues in large quantities of samples in the field of agricultural product safety. , it is difficult to popularize and apply to people's daily life

Method used

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  • Preparation method and use method of visual detection chip for multiple pesticide residues based on membrane material
  • Preparation method and use method of visual detection chip for multiple pesticide residues based on membrane material
  • Preparation method and use method of visual detection chip for multiple pesticide residues based on membrane material

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1: Preparation method of multi-residue visualization chip of six kinds of pesticides such as triazophos:

[0053] The solid phase support is a rectangular nitrocellulose membrane (Sartorius CN140, pore size 8 μm), with a size of 0.5 cm (width)×2 cm (length). The sample points on the solid phase carrier include positive control --- positive quality control point, which is goat anti-mouse IgG diluted with 0.01M PBS (pH7.4) to a mass concentration of 2 mg / mL; negative control --- negative quality control point, which is 0.01M PBS (pH7.4); the pesticide hapten-chicken ovalbumin (OVA) conjugates used in the detection point are the following 6 kinds: triazophos-OVA, parathion-OVA, methyl parathion -OVA, carbofuran-OVA, imidacloprid-OVA, chlorpyrifos-OVA), diluted with 0.01M PBS (pH7.4) to a concentration of 5-10mg / mL.

[0054] Spot the above-mentioned positive control, negative control, and diluted 6 kinds of pesticide hapten-chicken ovalbumin (OVA) conjugates on the...

Embodiment 2

[0073] Example 2, Preparation of 8 kinds of pesticide multi-residue visualization chips such as triazophos

[0074]Wherein the solid-phase carrier is a circular nitrocellulose membrane (Sartorius CN140, pore size 8 μm), with a diameter of 1 cm; the sample point on the solid-phase carrier includes a positive control-positive quality control point, which is a goat anti-mouse IgG with 0.01M PBS ( pH7.4) diluted to a mass concentration of 2mg / mL; negative control---negative quality control point, which is 0.01M PBS (pH7.4); the pesticide hapten used in the detection point-chicken ovalbumin (OVA) coupling The substances are the following 8 kinds: triazophos-OVA, parathion-OVA, methyl parathion-OVA, carbofuran-OVA, imidacloprid-OVA, fenpropathrin-OVA, acetamiprid-OVA, Chlorpyrifos-OVA, diluted with 0.01M PBS (pH7.4) to a concentration of 5-10mg / mL.

[0075] Spot the above-mentioned positive control, negative control, and diluted 8 kinds of pesticide haptens-chicken ovalbumin (OVA) ...

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Abstract

The invention discloses a preparation method of a visual detection chip for multiple pesticide residues based on a membrane material. The preparation method comprises the steps of spotting a detection point and quality-control points on a carrier to form a point array, the carrier being a nitrocellulose membrane; washing with a cleaning solution; drying in the air; sealing uncombined regions by using bovine serum albumin; washing with the cleaning solution; and drying in the air to obtain the visual detection chip for the multiple pesticide residues. The quality-control points comprises a positive quality-control point and a negative quality-control point; the positive quality-control point is goat anti mouse IgG; the negative quality-control point is 0.01 M phosphate buffer solution PBS (pH7.4); and the detection point is a pesticide hapten-chicken ovalbumin conjugate. The invention also provides a use method of the visual detection chip for the multiple pesticide residues. According to the invention, visualization of an enhanced gold mark color development and high throughput of a chip technology are integrated together, and an immune chip capable of determining by naked eyes and detecting a plurality of pesticide residues at the same time is prepared.

Description

technical field [0001] The present invention relates to a pesticide multi-residue visualization chip based on a membrane material and its preparation method and application method. Rapid qualitative and semi-quantitative detection of nicotinic pesticides. Background technique [0002] Pesticides are indispensable substances used in agricultural production to prevent and control diseases, insects, and weeds in crops, and play an extremely important role in ensuring and promoting stable agricultural production and income. However, due to long-term unreasonable use, the problem of pesticide pollution and multi-residue exceeding the standard has become more and more serious, and food safety issues have gradually become the focus of people's attention. At present, the detection of pesticide residues in my country mainly relies on instrumental analysis, such as gas chromatography (GC), liquid chromatography (LC), gas chromatography-mass spectrometry (GC-MS) and liquid chromatogra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/548
CPCG01N33/548G01N2430/10G01N2430/12
Inventor 郭逸蓉兰美静桂文君刘蕊朱国念
Owner ZHEJIANG UNIV
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