Visualized molecularly imprinted nanosensor and preparation and application thereof

A nano-sensor and molecular imprinting technology, applied in the fields of materials, analytical chemistry and food safety, can solve the problems of cumbersome synthesis process, and achieve the effect of simplifying the synthesis process, reducing the experimental cost and improving the mass transfer rate.

Active Publication Date: 2019-08-30
NANCHANG UNIV
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Problems solved by technology

However, the current synthetic process of the core-shell molecularly imprinted ratiometric fluorescent sensor is cumbersome and usually involves two steps. "Post-imprinting"; or prepare the core-shell type single-emission molecularly imprinted fluorescence sensing and embedded reference fluorescent silica nanoparticles separately, that is, "preparation first and then mixing"

Method used

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  • Visualized molecularly imprinted nanosensor and preparation and application thereof
  • Visualized molecularly imprinted nanosensor and preparation and application thereof
  • Visualized molecularly imprinted nanosensor and preparation and application thereof

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Embodiment 1

[0029] A preparation method of visualized molecularly imprinted nanosensor (see figure 1 ),Proceed as follows:

[0030] (1) 1g·L -1 Preparation of folic acid solution: first prepare 0.01mol L -1 Sodium hydroxide ethanol solution, in 500mL ethanol solution (volume fraction is 20%), add 200mg sodium hydroxide. Take 100mL of this solution and add 100mg of folic acid to get 1g·L-1 folic acid solution.

[0031] (2) Preparation of folic acid imprinted ratiometric fluorescent sensors (FL-MIPs) by sol-gel method: 8 mL of ultrapure water, 37 μLAPTES, and 8 mL of the above-mentioned folic acid were added to 1 mL of the silica nanoparticle solution, and stirred for 1 hour to form a prepolymerized mixture. Then add 50 μLTEOS and 50 μL ammonia water, and react for 10-12 hours in a dark environment. After the reaction, the product was centrifuged (8000rpm, 10 minutes), the supernatant was discarded, and then the template molecule folic acid in the precipitate was repeatedly eluted with ...

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Abstract

The present invention belongs to the fields of material, analytical chemistry and food safety, in particular to a molecularly imprinted ratiometric fluorescent nanosensor based on dual fluorescence emission, a preparation method and an application thereof in visual detection of folic acid in food. The surface of silica nanoparticles is subjected to imprinting polymerization with the sol-gel methodin one step; red fluorescent cadmium telluride quantum dots (CdTe QDs) and folic acid with spontaneous blue fluorescence are embedded; holes of eluted folic acid are used as recognition sites; then the dual fluorescence emission molecularly imprinted nanosensor with a core-shell structure is obtained, which is the visualized molecularly imprinted nanosensor. The preparation method of the invention is simpler than the conventional two-step method for preparing the molecularly imprinted ratiometric fluorescent sensor; cumbersome synthesis steps are avoided; and the experimental period is shortened. In addition, the sensor prepared by the method of the invention can be used for detecting folic acid with a high sensitivity, a high selectivity and self-correction; abundant fluorescence color evolution is provided; and the visual detection for a target is realized.

Description

technical field [0001] The invention belongs to the fields of materials, analytical chemistry and food safety, and in particular relates to a molecularly imprinted ratiometric fluorescence sensor based on dual fluorescence emission, a preparation method thereof and an application for visual detection of folic acid in food. Background technique [0002] Folic acid is a water-soluble vitamin that has important clinical significance to human health. As an important coenzyme factor, it plays an important physiological role in cell metabolism and participates in nucleic acid and red blood cell formation, cell replication and tissue growth. In addition, folic acid is a dietary supplement, and the current recommended daily intake for adults in the United States is 400 μg. The lack of folic acid in pregnant women can cause fetal neural tube defects; excessive intake of folic acid can affect the absorption of other nutrients, such as B 12 , zinc, which can cause other health proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N2021/6432
Inventor 熊华李楚瑶杨倩彭海龙朱雯婷
Owner NANCHANG UNIV
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