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Dual-emission fluorescent molecularly imprinted polymer nanoparticles and preparation method and application thereof

A dual emission fluorescence, molecular imprinting technology, applied in nanotechnology, nanotechnology, luminescent materials, etc., can solve the problems of dopamine human harm, complicated processing, expensive chromatography equipment, etc. fast effect

Active Publication Date: 2019-02-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The harm of abnormal dopamine (DA) content to the human body cannot be underestimated. At present, most of the methods for detecting dopamine are realized by chromatography, but the instruments used in chromatography are relatively expensive, and the pretreatment of sample detection is relatively complicated.

Method used

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  • Dual-emission fluorescent molecularly imprinted polymer nanoparticles and preparation method and application thereof
  • Dual-emission fluorescent molecularly imprinted polymer nanoparticles and preparation method and application thereof
  • Dual-emission fluorescent molecularly imprinted polymer nanoparticles and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0035] CdTe QDs were prepared by hydrothermal method. First prepare the precursor: prepare a clean centrifuge tube, mix 51 mg Te powder, 100 mg NaBH 4 Add 1.5 mL of double-distilled water to the centrifuge tube in turn, put a needle on the lid of the centrifuge tube to discharge the excess hydrogen from the reaction, and then place the centrifuge tube in an ultrasonic machine for ultrasonic reaction for 1.0 h to obtain the precursor of CdTe QDs NaHTe. 0.3651 g CdCl 2 ·2.5H 2 O and 0.1777 mL thioglycolic acid solution are mixed to form a mixed solution, add 100 mL distilled water to dilute, and use 1 mol / L NaOH solution to adjust the pH of the mixed solution to 11.2, pour in nitrogen gas and stir, half an hour later, the precursor NaHTe was quickly added to the mixed solution, and the reaction was refluxed in an oil bath at 140°C. Nitrogen was slowly introduced throughout the reaction. After 7 days of reaction, red fluorescent CdTe QDs were obtained.

[0036] Take 2.0 g citric ac...

Embodiment 2

[0039] First prepare the precursor: prepare a clean centrifuge tube, mix 40 mg Te powder, 80 mg NaBH 4 Add 1.2 mL of double-distilled water to the centrifuge tube in turn, insert a needle into the cap of the centrifuge tube to discharge the excess hydrogen from the reaction, and then place the centrifuge tube in an ultrasonic machine for ultrasonic reaction for 1.0 h to obtain the precursor of CdTe QDs NaHTe. 0.2 g CdCl 2 ·2.5H 2 O and 0.05 mL of thioglycolic acid solution were mixed to form a mixed solution, diluted with 50 mL of distilled water, and the pH of the mixed solution was adjusted to 9.5 with 1 mol / L NaOH solution, and argon was introduced and stirred. After half an hour, the precursor NaHTe was quickly added to the mixed solution, and the reaction was refluxed in an oil bath at 140°C. The argon gas was slowly introduced throughout the reaction. After 7 days of reaction, red fluorescent CdTe QDs were obtained.

[0040] Take 2.0 g citric acid, 1.0 g polyethyleneimine a...

Embodiment 3

[0043] CdTe QDs were prepared by hydrothermal method. First prepare the precursor: prepare a clean centrifuge tube, mix 60 mg Te powder, 120 mg NaBH 4 Add 1.8 mL of double-distilled water to the centrifuge tube in turn, put a needle on the lid of the centrifuge tube to discharge the excess hydrogen in the reaction, and then place the centrifuge tube in an ultrasonic machine for ultrasonic reaction for 1.0 h to obtain the precursor of CdTe QDs NaHTe. Add 0.5g CdCl 2 ·2.5H 2 O and 0.25mL thioglycolic acid solution were mixed into a mixed solution, diluted with 500mL distilled water, and the pH of the mixed solution was adjusted to 12 with a NaOH solution with a concentration of 1 mol / L. Nitrogen was introduced and stirred. After half an hour, the precursor NaHTe was quickly added to the mixed solution, and the reaction was refluxed in an oil bath at 140°C. Nitrogen was slowly introduced throughout the reaction. After 7 days of reaction, red fluorescent CdTe QDs were obtained.

[0...

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Abstract

The invention relates to the technical field of preparation of bio-functional materials, in particular to dual-emission fluorescent molecularly imprinted polymer nanoparticles and a preparation methodand application thereof. carbon quantum dots are coated with silica nanospheres to form a core of a ratio fluorescence probe; red cadmium telluride quantum dots are used as response signals, acrylamide and 4-vinylbenzeneboronic acid are used as bifunctional monomers, N,N-methylene bisacrylamide is used as a crosslinker, dopamine is used as templating molecules, dual-emission fluorescent molecularly imprinted polymer nanoparticles having specific recognition sites for dopamine are synthesized in an alcohol phase under initiating of azodiisobutyronitrile, and fluorescent detection test paper with visualizing effect is prepared then by means of impregnating so as to provide visual detection for DA (dopamine); the fluorescent detection test paper is applied to the detection of DA in a human serum sample, and the results prove that the fluorescent detection test paper prepared herein is suitable for half-quantitative detection of DA in actual complex samples.

Description

Technical field [0001] The invention relates to the technical field of biological functional material preparation, in particular to a dual-emission fluorescent molecularly imprinted polymer nanoparticle, and a preparation method and application thereof. Background technique [0002] Molecular imprinting technology (Molecular imprinting technology, MIT) is a process of preparing polymers with specific recognition ability for a specific molecule, and can be used to prepare molecularly imprinted polymers (MIPs) with specific selectivity. Fluorescence analysis has the advantages of fast, simple, sensitive, etc., and has great potential in the field of analysis. Compared with high-performance liquid chromatography and other advanced instrument analysis, it is relatively large in terms of solvent consumption, sample pre-processing, and test time. The advantages. [0003] More and more scientific researchers choose quantum dots as the emission source of fluorescent signals, and use the s...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/06C09K11/65C09K11/59C09K11/88B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00C09K11/025C09K11/06C09K11/592C09K11/65C09K11/883G01N21/6428G01N2021/6439
Inventor 王吉祥徐叶青潘国庆闫永胜
Owner JIANGSU UNIV
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