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Preparation method and Cu<2+> detection application of rare earth ratiometric fluorescent probe

A ratiometric fluorescent probe and ratiometric fluorescence technology, applied in the field of environmental detection, to achieve the effect of improved accuracy, high sensitivity and selective detection

Active Publication Date: 2018-09-11
RUIJIN SHENGYUAN METAL NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, for Cu 2+ Most of the fluorescence responses are based on single fluorescence quenching probes, which are often limited by light source or detector drift or complex sample environmental factors, etc.

Method used

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  • Preparation method and Cu&lt;2+&gt; detection application of rare earth ratiometric fluorescent probe
  • Preparation method and Cu&lt;2+&gt; detection application of rare earth ratiometric fluorescent probe
  • Preparation method and Cu&lt;2+&gt; detection application of rare earth ratiometric fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of luminol-Tb-GMP ratiometric fluorescent probe: Mix 40 μL of 10 mM luminol solution with 90 μL of 100 mM guanosine monophosphate solution, stir thoroughly for 30 minutes, then add 100 μL of 100 mM Tb(NO 3 ) 3 ·6H 2 O solution, continue to stir and react for 30 minutes to obtain a milky white flocculent precipitate; centrifuge the milky white flocculent precipitate at a speed of 12000rpm, wash the obtained precipitate with ultrapure water and then centrifuge again, repeat 3 times, and dissolve the obtained precipitate in 1mL In ultrapure water, make a rare earth ratio fluorescent probe solution.

[0022] Luminol, luminol-Tb, GMP-Tb, luminol-Tb-GMP and luminol-GMP were characterized by fluorescence spectroscopy, the results were as follows figure 1 shown. in, figure 1 Fluorescence spectra of luminol, luminol-Tb, GMP-Tb, luminol-Tb-GMP and luminol-GMP. Under the excitation wavelength of 290nm, the maximum fluorescence emission peak of luminol is located a...

Embodiment 2

[0027] luminol concentration, Tb 3+ Optimization of concentration and assay pH

[0028] For the luminol concentration and Tb in the prepared luminol-Tb-GMP solution 3+ Concentration and the Cu 2+ The experimental conditions such as the pH value of the solution during detection were optimized. The concentration of luminol plays an important role in the sensing process, it not only affects the effect of luminol-Tb-GMP on Cu 2+ Sensitivity and selectivity, also play the role of internal reference signal. When no Cu 2+ When, with the increase of luminol concentration in luminol-Tb-GMP, F 430 / F 547 The value of gradually increases; when adding Cu 2+ , due to Cu 2+ Quenching Tb 3+ The fluorescence makes F 430 / F 547 value increases, and the concentration of luminol in luminol-Tb-GMP is 0.4mM on Cu 2+ The response is the most sensitive, therefore, the optimal concentration of luminol in the prepared luminol-Tb-GMP solution is 0.4mM. When no Cu 2+ , with Tb in luminol-T...

Embodiment 3

[0030] luminol-Tb-GMP on Cu 2+ detection application

[0031] Under optimized experimental conditions, the luminol-Tb-GMP ratiometric fluorescent probe was used for Cu 2+ Perform quantitative testing. Mix 10 μL luminol-Tb-GMP solution, 40 μL 10 mM pH 9.0 Tris-HCl buffer solution and different concentrations of Cu 2+ The solutions were mixed, diluted with ultrapure water to a total volume of 200 μL, reacted at 37° C. for 30 minutes, and measured the fluorescence spectrum of the solution when the excitation wavelength was 290 nm. Depend on Figure 5 It can be seen that the luminol-Tb-GMP ratiometric fluorescent probe simultaneously emits luminol and Tb 3+ double fluorescent signal; with Cu 2+ As the concentration increases, the fluorescence intensity of luminol at 430nm remains unchanged, and Tb 3+ The fluorescence intensity at 547nm gradually weakens, then luminol and Tb 3+ The fluorescence signal ratio F 430 / F 547 Gradually increase ( Figure 5 A), F 430 / F 547 wi...

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Abstract

The invention discloses a preparation method and a Cu<2+> detection application of a rare earth ratiometric fluorescent probe, and belongs to the technical field of environmental detection. The preparation method of the luminol-Tb-GMP fluorescent probe is established through the self-polymerization coordination effect among luminol, GMP and Tb<3+> by using luminol and guanosine monophosphate (GMP)as dual ligands and using a rare earth ion Tb<3+> as a luminescent center ion. The luminol-Tb-GMP fluorescent probe combines the dual fluorescent signals of luminol and Tb<3+>, and simultaneously emits dual fluorescent signals of the luminol and Tb<3+> under the same excitation wavelength. When Cu<2+> exists, the luminol and GMP have a strong coordination effect on the Cu<2+> in order to preventelectrons from being transferred from the amino group of the GMP to Tb<3+>, so fluorescence quenching of the Tb<3+> is caused, and the fluorescence intensity of the luminol is unchanged, thereby a Cu<2+> detection method based on the fluorescence signal ratio of the luminol and Tb<3+> is established. Additionally, the green fluorescence of the Tb<3+> gradually weakens and the blue fluorescence ofthe luminol gradually appears with the increase of the Cu<2+> concentration, so the visual detection of the Cu<2+> can be achieved. The luminol-Tb-GMP fluorescent probe also can be applied to the sensitive detection of the Cu<2+> in an environmental water samples and a biological sample.

Description

technical field [0001] The invention relates to a preparation method of a rare earth ratio fluorescent probe and its Cu 2+ The detection application belongs to the technical field of environmental detection. Background technique [0002] Rare earth coordination polymers (Ln-CPs) have attracted more and more attention due to their excellent optical properties such as high fluorescence quantum yield, long fluorescence lifetime, large stoke shift, linear emission spectrum, and adjustable composition and nanometer size. . In recent years, fluorescent probes based on Ln-CPs have been widely used in the sensing of small molecules, cations, anions, temperature and humidity, and pH (Real-time Ratiometric Fluorescent Assay for Alkaline Phosphatase Activity with Stimulus Responsive Infinite Coordination Polymer Nanoparticles, Analytical Chemistry, 2015, 87, 3080-3086). Studies have shown that most of the bridging ligands constituting Ln-CPs are synthetic organic molecules. These or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C08G83/00G01N21/64G01N21/78
CPCC08G83/008C09K11/06C09K2211/182G01N21/643G01N21/6486G01N21/78
Inventor 邱建丁童圆君梁汝萍
Owner RUIJIN SHENGYUAN METAL NEW MATERIAL CO LTD
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