Method for detecting mercury ions by nucleic acid aptamer based photoelectrochemistry

A nucleic acid aptamer and photoelectrochemical technology, applied in the field of analysis and testing, can solve the problems of low sensitivity, high toxicity of reagents, expensive equipment, etc., and achieve the effect of increasing the production rate, good specificity, and promoting effective separation

Inactive Publication Date: 2014-02-05
ANHUI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, each method has at least one of the following disadvantages, such as expensive equipment, complicated methods, time-consuming, low sensitivity, and high toxicity of reagents.

Method used

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  • Method for detecting mercury ions by nucleic acid aptamer based photoelectrochemistry
  • Method for detecting mercury ions by nucleic acid aptamer based photoelectrochemistry

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Embodiment 1

[0022] like figure 1 As shown, the preparation method of perylenetetracarboxylic acid / graphene oxide heterojunction is as follows: First, 2 × 10 -4 mol L -1 of perylenetetracarboxylic acid DMF solution and 2 mg mL -1 Then, the perylene tetracarboxylic acid solution and the graphene oxide solution are fully mixed in a volume ratio of 10:1, and allowed to stand, and a perylene tetracarboxylic acid / graphene oxide heterojunction is formed.

[0023] The glassy carbon electrode was polished with a 0.05 μm particle size aluminum oxide suspension, washed with acetone and pure water in turn, and then left to dry at room temperature. 10 μL of perylene tetracarboxylic acid / graphene oxide heterojunction solution was drop-coated on the surface of glassy carbon electrode and dried at room temperature to prepare perylene tetracarboxylic acid / graphene oxide heterojunction modified electrode. Next, the aminated T bases were immobilized on the surface of the perylenetetracarboxylic acid / grap...

Embodiment 2

[0025] The preparation method of perylenetetracarboxylic acid / graphene oxide heterojunction is as follows: First, prepare 1 × 10 -4 mol L -1 perylenetetracarboxylic acid DMF solution and 1 mg mL -1 Then, the perylene tetracarboxylic acid solution and the graphene oxide solution are fully mixed in a volume ratio of 5:1, and allowed to stand, and a perylene tetracarboxylic acid / graphene oxide heterojunction is formed.

[0026] The glassy carbon electrode was polished with a 0.05 μm particle size aluminum oxide suspension, washed with acetone and pure water in turn, and then left to dry at room temperature. 5 μL of the perylene tetracarboxylic acid / graphene oxide heterojunction solution was drop-coated on the surface of the glassy carbon electrode and dried at room temperature to obtain the perylene tetracarboxylic acid / graphene oxide heterojunction modified electrode. Next, the aminated T bases were immobilized on the surface of the perylenetetracarboxylic acid / graphene oxide ...

Embodiment 3

[0028] The preparation method of perylenetetracarboxylic acid / graphene oxide heterojunction is as follows: First, prepare 0.5×10 -4 mol L -1 perylenetetracarboxylic acid DMF solution and 0.5mg mL -1 Then, the perylene tetracarboxylic acid solution and the graphene oxide solution are fully mixed at a volume ratio of 2.5:1, and allowed to stand, and a perylene tetracarboxylic acid / graphene oxide heterojunction is formed.

[0029] The glassy carbon electrode was polished with a 0.05 μm particle size aluminum oxide suspension, washed with acetone and pure water in turn, and then left to dry at room temperature. 2.5 μL of perylene tetracarboxylic acid / graphene oxide heterojunction solution was drop-coated on the surface of glassy carbon electrode and dried at room temperature to prepare perylene tetracarboxylic acid / graphene oxide heterojunction modified electrode. Next, the aminated T bases were immobilized on the surface of the perylenetetracarboxylic acid / graphene oxide hetero...

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Abstract

The invention relates to a method for detecting mercury ions by nucleic acid aptamer based photoelectrochemistry. An On-Off type nucleic acid aptamer based photoelectrochemical sensor is adopted for detecting the mercury ions with different concentrations and is constructed based on quercetin copper sensitization perylene tetracarboxylic-oxidized graphene heterojunction. Based on double advantages of a quercetin copper compound as a photosensitizer and a reducing agent, the method can be used for double amplifying photoelectrical signals, and has the characteristics of being convenient, simple, super sensitive, economical, high in specificity and the like. The constructed On-Off type nucleic acid aptamer based photoelectrochemical sensor adopts current-time technology to detect the mercury ions with different concentrations in a 0.1 mol/L phosphate buffered solution at the offset electric potential of 0.2V and under optical radiation of more than 450 nm.

Description

technical field [0001] The invention relates to the field of analysis and testing, in particular to a method for detecting mercury ions that can be applied to environmental monitoring. Background technique [0002] Mercury ions are one of the common pollutants, which can be transformed into highly toxic methylmercury through the action of microorganisms, and enter the human body through the food chain, causing mercury poisoning. At present, there are methods for detecting mercury ions, such as atomic spectroscopy, molecular spectroscopy, ion chromatography, electrochemical methods and immunoassays. However, each method has at least one of the following disadvantages, such as expensive equipment, complex method, time-consuming, low sensitivity, and high toxicity of reagents. Therefore, it is especially necessary to invent new analytical methods to overcome the above-mentioned disadvantages. Photoelectrochemical detection is a novel test method, which is based on the separat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
Inventor 薛延石建军李红波王伟
Owner ANHUI UNIV OF SCI & TECH
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