Mercury-ion detection method simulating peroxidase based on nano platinum and kit
A peroxidase and detection kit technology, applied in the fields of analytical chemistry and nanometers, can solve the problems of unfavorable rapid analysis of low-concentration mercury detection, troublesome pretreatment analysis technicians, complicated operations, etc., and achieves a simple, fast, and easy preparation process. Obtain and test the effect of high sensitivity
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Embodiment 1
[0037] Mix 1 ml of 16 mmol / L chloroplatinic acid aqueous solution with 1 ml of 40 mmol / L trisodium citrate, dilute with 38 ml of double distilled water, and stir for 30 minutes in the dark. Subsequently, 0.2 ml of 50 mmol / L sodium borohydride aqueous solution was added (the addition time was controlled within 2 minutes), the color of the reaction solution changed from light yellow to brownish yellow, and stirring was continued for 1 hour in the dark. The resulting nano-platinum concentration was 78 mg / L, and the average particle size was 2.5±0.4 nm. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.
Embodiment 2
[0039] The nano-platinum solution obtained in Example 1 was diluted 50 times with double distilled water to obtain a nano-platinum solution with a concentration of 1.56 mg / L. Take 0.1 ml of nano-platinum solution with a concentration of 1.56 mg / L, add 2.7 ml of double distilled water, 1 ml of hydrogen peroxide with a concentration of 2 mol / L, and 0.2 ml of 3,3',5 , 5'-Tetramethylbenzidine hydrochloride, after mixing, bathe in water at 45°C for 10 minutes, the color of the system will be dark blue, and the maximum absorption wavelength of the system is at 652 nm (see figure 1 ).
Embodiment 3
[0041] The nano-platinum solution obtained in Example 1 was diluted 50 times with double distilled water to obtain a nano-platinum solution with a concentration of 1.56 mg / L. In 0.1 ml of nano-platinum solution with a concentration of 1.56 mg / L, add 0.4 ml of a mercury ion sample solution with a concentration of 50 nmol / L, mix and place it at room temperature for 2 minutes, add 2.3 ml of double distilled water, 1 ml of hydrogen peroxide with a concentration of 2 mol / L and 0.2 ml of 3,3',5,5'-tetramethylbenzidine hydrochloride with a concentration of 16 mmol / L were mixed in a water bath at 45°C for 10 minutes, and the system It is light blue after color development, and the absorption value of this system at 652 nm is significantly lower than that of Example 2 (see figure 2 ).
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