67results about How to "Improve selective recognition" patented technology

The invention provides a preparation method of a fluorescent probe and an application of the fluorescent probe in aluminum ion detection. The fluorescent probe is obtained by virtue of a reaction in a heating reflux manner between 2-hydroxyl-1-naphthaldehyde and carbohydrazide. In the structure of the fluorescent probe provided by the invention, the N atoms on the C=N double bonds are influenced by a photo-induced electron transfer effect, and after the N atoms are combined with aluminum ions, the photo-induced electron transfer effect is inhibited, and planar conjugation is enhanced at the same time; two mechanisms together induce obvious enhancement of fluorescence emission; the fluorescent probe has good selectivity and sensitivity to the aluminum ions. High-selectivity identification of the aluminum ions is realized, a relatively good linear relation exists in a range of 5*10<-6> to 4*10<-5> mol/L, and the limit of detection is as low as 1.89*10<-8> mol/L, so that the fluorescent probe has a chromogenic sensing function.

The invention relates to a method for preparing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence sensor array used for detecting an organic chlorine pesticide. The marking recognition sites in the fluorescence sensor array are mutually effected with organic chlorine pesticide molecules (target molecules), the target molecules and Tb<3+> on the recognition sites form an organic ligand through coordination, and change of the fluorescence intensity of Tb<3+> is used for detecting the target molecules. The method comprises the following three steps: preparing Y2O3:Tb<3+> fluorescent powder, then synthesizing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence probe having capability for identifying the marking target molecules, finally, through a micromachining technology and a plasma etching method, dropping the fluid suspension of the fluorescence probe having the capability for identifying the marking target molecules on a silicon chip, naturally drying the silicon chip, and spontaneously putting the fluorescence probe materials in a micro hole to obtain the fluorescence sensor array for high-selectivity identification and high sensitive signal detection on the target molecules.

The invention relates to a method for preparing a Eu3+ labelled molecule imprinted sensor used for pesticide residue detection and belongs to the technical field of environment functional material preparation. The method comprises the steps that Eu3+ and amino groups and pesticide residue molecules in APTS are pre-organized and then subjected to hydrolytic crosslinking condensation with TEOS so that the Eu3+ labelled pesticide residue molecule imprinted silicon dioxide nanoparticle sensor can be obtained, pesticide residue molecule selective recognition site holes are obtained after pesticide residue molecules are eluted, chelation between pesticide residue molecules and Eu3+ on a recognition site is conducted after the pesticide residue molecules enter the recognition site of the sensor again, the fluorescence intensity of the pesticide residue molecules is improved after being chelated with Eu3+, and then high-selectivity, high-binding-capacity and high-sensitivity detection of the trace pesticide residue molecules is achieved by means of the change of the fluorescence intensity. The obtained Eu3+ labelled molecule imprinted sensor has the advantages that the rigidity of the recognition site is high, Eu3+ is not prone to elution, repeated utilization can be achieved, solvent inertness, light stability and single dispersity are high, and size is uniform.

The invention discloses a preparation method of compound (I) 4-hydroxy substituted phenyl imine type rhodamine 6G fluorescence molecule probe and purpose thereof. The compound I has the advantages of rhodamine fluorescence dye, and simultaneously can emit strong fluorescence in the highly acid environment, and the solution color is changed from achromatic color to orange red. Therefore, the compound I has the purposes of naked eye color developing identification and fluorescence identification with high selectivity to hydrogen ion.

The invention provides a preparation method of a SrAlB2O7:Eu<3+> fluorescent probe for detection of OPs (organophosphorus pesticides). The preparation method comprises two steps as follow: firstly, Eu<3+> and target analyte, OP molecules, interact to form organic ligand, then strontium nitrate, aluminum nitrate, boric acid and a pore-forming agent are added to a beaker accommodating deionized water, the mixture is stirred, and sol is formed; calcination is performed after gel is obtained, and Eu<3+> marked OP molecular printing SrAl2B2O7:Eu<3+> fluorescent probe is obtained. OP molecule selectivity recognition sites are available after template molecules are removed, the OP molecules further interact with Eu<3+> on the recognition sites after entering the recognition sites of the fluorescent probe, the OP molecules and the recognition sites form organic ligand, the organic ligand absorbs energy and transfers the energy to Eu<3+>, the fluorescence intensity of the fluorescent probe is changed, and selective recognition and detection of pesticide molecules are realized.

The invention discloses a preparation method and application of a fluorescent probe based on benzothiazole Schiff base, belonging to the technical field of fluorescent probes for metal ion detection. The molecular formula of the fluorescent probe is C34H31N5O2S2. The preparation method comprises the following steps: (1) subjecting 2-hydroxy-5-methylbenzaldehyde with 2-aminothiophenol to reacting to obtain a compound a; (2) carrying out the Darff reaction on the compound a and hexamethylenetetramine to generate a compound b; and (3) carrying out a condensation reaction on the compound b and diethylenetriamine to obtain a probe molecule L. The probe molecule reacts with zinc ions in a solution containing both absolute ethyl alcohol and Tris-HCl in a ratio of 3: 2, and the concentration of the zinc ions can be detected by utilizing the change of fluorescence intensity. The probe disclosed by the invention is simple and convenient to synthesize, mild in reaction conditions and high in selectivity and sensitivity to zinc ions, the response range of the probe is 0-8.00 [mu]M, and the detection limit of the probe is 60.4 nM. In addition, through a laser confocal scanning microtechnique, the novel fluorescent probe is successfully applied to detection of zinc ions in cells.

The invention discloses a cyanide ion fluorescence sensor taking N,N-diethyl salicylaldehyde as a fluorescence signal group and acylhydrazone as a recognition site. The sensor is prepared by performing esterification and hydrazinolysis on 2-hydroxy-3-naphthoic acid and reacting with N,N-diethyl salicylaldehyde. The recognition effect of the sensor for F-, Cl-, Br-, I-, AcO-, H2PO4-, HSO4-, ClO4-, CN- and SCN- totally ten kinds of negative ions is studied by the colorimetric method and fluorescent spectrometry, and indicates that the sensor molecule can perform single selection recognition on cyanide ions in a water-containing system and has relatively high sensitivity on CN- detection, and the recognition process is not interfered by other negative ions. The invention further provides CN- detection test paper based on the sensor molecule. The test paper can be used for conveniently and quickly detecting CN- in a water-containing medium. The sensor molecule FC which is designed and synthesized by the invention is simple in structure, simple and convenient in synthesizing method and low in cost, and has a good application prospect in CN- detection.

The invention belongs to the technical field of covalent organic framework functional materials, particularly relates to preparation and application of an amino-functionalized hydrazone covalent organic framework material, and aims to develop a novel amino-functionalized sensing material which has a strong fluorescence effect and can be used as a fluorescent probe for metal ion recognition. The preparation method comprises the following steps: firstly designing and synthesizing an amino-functionalized dihydrazide monomer containing functional groups with different reaction activities, and then carrying out acetic acid catalytic reaction on the amino-functionalized dihydrazide monomer and a benzenetricarboxaldehyde monomer in an organic solvent under the protection effect of inert gas. The prepared amino-functionalized hydrazone covalent organic framework material has the advantages of rich amino functional sites, good crystallinity, high stability and the like, has strong fluorescence, and can be used as a fluorescent probe for high-sensitivity and high-selectivity recognition of Fe (III) ions.

The invention belongs to the technical field of covalent organic framework functional materials, particularly relates to preparation of a hydrazone chiral covalent organic framework material and an application of the hydrazone chiral covalent organic framework material in metal ion recognition, and aims to develop a novel sensing material which has strong fluorescence and can be used as a fluorescent probe for the metal ion recognition. The hydrazone chiral covalent organic framework material is obtained through an acetic acid catalytic reaction of tris (4-formyl phenyl)amine and chiral hydrazide monomers in an organic solvent under the protection action of inert gas, and the prepared hydrazone chiral covalent organic framework material has the advantages of being good in crystallinity, high in stability and the like, has strong fluorescence and can be used as a fluorescent probe for recognizing Fe (III) ions. Meanwhile, the hydrazone chiral covalent organic framework material is bonded to an amino-modified QCM wafer through an in-situ growth method, and a COF-based QCM sensor capable of achieving high-selectivity recognition of Fe (III) is prepared.

The invention relates to a preparation method for an artificial antibody for caffeine detection. Imprinted molecules located in an imprinted sphere layer are eluted with a prepared artificial antibody, so that a hole structure of which the structure, size and functional group are complementary to the structure, size and functional group of the imprinted molecules is formed in the imprinted spherelayer; the artificial antibody has specific recognition sites for enzyme-labeled antigen molecules, so that the artificial antibody can selectively recognize the enzyme-labeled antigen molecules; anda chemical immunoluminescence method is used to perform trace detection on the enzyme-labeled antigen molecules with a detection limit being 5.2*10<-11> mol.L<-1>. The preparation process of the artificial antibody includes the following four steps that: a horseradish peroxidase is oxidized; a horseradish peroxidase-labeled antigen is prepared, a caffeine antigen of a certain concentration gradient is configured, and the oxidized horseradish peroxidase is added dropwise, an enzyme-labeled antigen having the largest luminescence value is selected; a target molecular imprinted polymer is prepared with imprinted molecules, functional monomer, a cross-linking agent, and an initiator; and the target molecules are eluted, so that an artificial antibody capable of selectively recognizing caffeinecan be obtained.

The invention discloses a preparation method for a cubic nanogold SERS probe used for TNT detection. The preparation method comprises seed crystal gold preparation, further growth and the functional modification and the self assembling of a silicon wafer. The preparation process of the preparation method comprises the following steps that: firstly, preparing seed crystal gold, and enabling the seed crystal gold to further grow; secondly, through a seed growth mechanism, strictly controlling the growth environment of the seed crystal gold to enable the seed crystal gold to grow into a cubic structure; thirdly, modifying sulfydryl on a polished 1*1cm<2> silicon wafer to enable a gold particle monolayer to be assembled to the surface of the silicon wafer on the own; and finally, modifying thesurface of the silicon wafer with electron-rich amino, realizing the selective recognition of the TNT further through a static electricity mutual function with three electron deficiency nitryls in aTNT target molecule, and utilizing a local area plasma resonance field on the surface of the nanogold to amplify and enhance the conventional Raman signal of the TNT molecule so as to realize the trace detection of the TNT, wherein a detection limit is 10<-9>mol.L<-1>. The preparation method has the advantages of being simple in preparation, convenient in operation, low in cost, good in selectivity and high in sensitivity.

The invention discloses the one-step hydrothermal synthesis based on ionic liquid novel fluorescent carbon dots and the detection application of one-step hydrothermal synthesis for sulfathiazole. Theone-step hydrothermal synthesis is characterized by taking 1-allyl-3-vinyl imidazole tetrafluoroborate as a carbon source, the malonic acid as a passivating agent, and synthesizing the novel fluorescent carbon dots capable of specifically recognizing sulfathiazole through a high-temperature hydrothermal reaction. A novel sulfathiazole fluorescent probe synthesized by the invention is simple in preparation process, low in cost and high in selectivity, has high sensitivity and accuracy in the detection of the trace sulfathiazole remained in an environmental sample, and has a wide application prospect in the field of analysis and detection of sulfathiazole.

The invention discloses a preparation method of a molecularly imprinted open tubular column, and relates to the technical field of new materials. The preparation method of the molecularly imprinted open tubular column comprises the following steps: a molecularly imprinted polymer is prepared by taking p-hydroxy-benzoate ester as template molecules and subjected to epoxy group modification, and theepoxy modified molecularly imprinted polymer is obtained; the surface of a capillary tube is subjected to amino modification, and an amino-modified capillary tubular column is obtained; the epoxy modified molecularly imprinted polymer is dispersed in ethyl alcohol to form a mixed solution; and the mixed solution is injected into the amino-modified capillary tubular column, sealing and polymerization are conducted, and the molecularly imprinted open tubular column is formed. The molecularly imprinted open tubular column is prepared, and the molecularly imprinted open tubular column can analyzethe p-hydroxy-benzoate ester in a complex sample.

The invention discloses a paper base dual-imprinting material capable of selectively recognizing a protein as well as a preparation method and an application of the paper base dual-imprinting material. The preparation method comprises the steps of immobilizing a graphene oxide-hemin compound on the surface of a filter paper material and preparing the dual-imprinting material on the surface of filter paper. The paper base dual-imprinting material is used for selectively recognizing and quantitatively detecting the protein. The paper base dual-imprinting material has the characteristics of low cost, environmental protection, simplicity in operation, high recyclability, and specific recognition of the protein, and particularly has the excellent characteristics of color development, water retention and hydrophily, and can rapidly detect the protein macromolecule at high throughput.

The invention discloses a method for improving the economy of 5G differential protection based on disturbance identification, and the method comprises the steps: in the 5G differential protection of a distribution network line, in a normal state, sending a normal message to other sides through the differential protection of the local side every T1; when disturbance occurs, the slave side continuously sends full data messages to the host side; according to the method, the situation that the power distribution network has a certain probability of disturbance and fault occurrence during normal operation and sends the synchronous state and the state quantity related to differential motion at regular time in a normal state is considered, and the full-data message is continuously sent only when disturbance, abnormity or fault occurs, so that the monthly average flow of 5G differential motion is reduced to the maximum extent, the monthly average flow of 5G differential motion can be effectively reduced, and the service life of the power distribution network is prolonged. A large amount of popularization and application of 5G differential motion in the power distribution network are promoted, the selective fault recognition capability of the power distribution network is improved, and therefore the problems that the power distribution network multi-end power line fault cannot be accurately recognized and the current power distribution network line relay protection selectivity is not high are solved.