Method for preparing Eu3+ labelled molecule imprinted sensor used for pesticide residue detection

A technology of labeling molecules and molecular imprinting, which is applied in the field of materials science, can solve the problems of slow binding kinetics, small number of recognition sites, and no signal output, etc., and achieve highly sensitive trace detection, high selectivity and trace detection Effect

Active Publication Date: 2015-12-09
HEFEI UNIV
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Problems solved by technology

[0015] The purpose of the present invention is to solve the shortcomings of the traditional molecular imprinting materials in the current prior art, such as the small number of recognition sites, slow binding kinetics, poor sensitivity and no signal output after binding target analytes. The present invention utilizes Eu 3+ Ions can coordinate with the electron-rich amino group in 3-aminopropyltriethoxysilane (3-aminopropyltriethoxysilane, APTS) and the target analyte molecule (pesticide molecule). Under alkaline conditions, 3-aminopropyl Hydrolysis of triethoxysilane and ethyl orthosilicate yields silica nanoparticles with Eu bound in the particle matrix 3+ Ions and amino groups, target analyte coordination chelation derivatives, target analyte molecules after chelation Eu 3+ Enhanced fluorescence intensity of ions, Eu in silica nanoparticle matrix 3+ Ion coordination and chelation form a molecularly imprinted sensor with fluorescent properties. Therefore, the application of rare earth chelation luminescence and molecular imprinting technology in the detection of pesticide residues can be explored

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  • Method for preparing Eu3+ labelled molecule imprinted sensor used for pesticide residue detection
  • Method for preparing Eu3+ labelled molecule imprinted sensor used for pesticide residue detection
  • Method for preparing Eu3+ labelled molecule imprinted sensor used for pesticide residue detection

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specific Embodiment approach

[0054] detailed description: A kind of Eu for the detection of pesticide residues 3+ Preparation method of labeled molecularly imprinted sensor, including Eu 3+ Pre-assembled with 3-aminopropyltriethoxysilane (3-aminopropyltriethoxysilane, APTS) and the pesticide target analyte chlorpyrifos molecule, after hydrolysis and condensation to obtain Eu 3+ The labeled chlorpyrifos molecularly imprinted silica nanoparticle sensor is characterized in that: the Eu 3+ After the template molecule (the target analyte chlorpyrifos molecule) is eluted from the labeled chlorpyrifos molecularly imprinted silica nanoparticle sensor, it has a selective recognition site for the target analyte chlorpyrifos molecule, and the target analyte chlorpyrifos molecule enters the silica After the recognition site of the nanoparticle sensor, it will be further combined with the Eu on the recognition site 3+ The ions are chelated, based on the principle of rare earth chelation luminescence, the target an...

Embodiment 1

[0058] Example 1: According to the principle of rare earth chelation luminescence, using Eu 3+ Chelate with the target molecule and the amino group in APTS, and obtain Eu 3+ labeled molecularly imprinted SiO 2 Nanoparticle sensor. Specific steps are as follows:

[0059] Weigh 0.0312g of Eu 2 o 3 Place in a 25mL beaker, add 250μL of concentrated nitric acid and 1300uL of deionized water to it, and sonicate for 7min, and when the solution is clarified, Eu(NO 3 ) 3 solution. Take 0.0368g of chlorpyrifos and ultrasonically dissolve it in ethanol solution, then add 240 μL of APTS to the solution, then add this solution to the previously prepared Eu(NO 3 ) 3 solution, and then put the solution in a 250mL round bottom flask, ultrasonic 9min, after standing still for 28min, add 2.9mL TEOS, adjust the pH to 10 with ammonia water, add 90mL ethanol water to adjust the volume of the solution to 100mL. The solution was placed on a magnetic stirrer, stirred at 750rpm for 3min, and ...

Embodiment 2

[0060] Embodiment 2: Eu by weighing 0.0180g 2 o 3 Place in a 25mL beaker, add 240μL of concentrated nitric acid and 1300uL of deionized water to it, and ultrasonicate for 6 minutes. After the solution is clarified, Eu(NO 3 ) 3 solution. Take 0.0240 g of imidacloprid and ultrasonically dissolve it in an ethanol solution, then add 220 μL of APTS to the solution, and then add this solution to the Eu(NO 3 ) 3 solution, and then put the solution in a 250mL round bottom flask, ultrasonic 9min, after standing for 30min, add 2.5mL of TEOS, adjust the pH to 10 with ammonia water, add 85mL of ethanol water to adjust the volume of the solution to 100mL, put the solution in On a magnetic stirrer, stir at 750rpm for 5min, then gently stir (at a fixed speed of 500rpm) for 5h, the resulting solution is evenly distributed in three 50mL centrifuge tubes, and centrifuged to obtain the hydrolyzed imprinted polymer. Take 90mL of concentrated nitric acid, put it into a 500mL volumetric flask,...

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Abstract

The invention relates to a method for preparing a Eu3+ labelled molecule imprinted sensor used for pesticide residue detection and belongs to the technical field of environment functional material preparation. The method comprises the steps that Eu3+ and amino groups and pesticide residue molecules in APTS are pre-organized and then subjected to hydrolytic crosslinking condensation with TEOS so that the Eu3+ labelled pesticide residue molecule imprinted silicon dioxide nanoparticle sensor can be obtained, pesticide residue molecule selective recognition site holes are obtained after pesticide residue molecules are eluted, chelation between pesticide residue molecules and Eu3+ on a recognition site is conducted after the pesticide residue molecules enter the recognition site of the sensor again, the fluorescence intensity of the pesticide residue molecules is improved after being chelated with Eu3+, and then high-selectivity, high-binding-capacity and high-sensitivity detection of the trace pesticide residue molecules is achieved by means of the change of the fluorescence intensity. The obtained Eu3+ labelled molecule imprinted sensor has the advantages that the rigidity of the recognition site is high, Eu3+ is not prone to elution, repeated utilization can be achieved, solvent inertness, light stability and single dispersity are high, and size is uniform.

Description

Technical field [0001] The invention involves the field of material science, and specially involves EU with the detection of pesticide residues of traces 3+ Method Molecular Seal Sensor Preparation Method. Background technique [0002] From the perspective of social development, the generation and development of pesticides have greatly promoted the pace of human society.However, from the perspective of environmental protection and food safety, pesticides have the characteristics of high residues and high stability, which can not be underestimated by human health. Therefore, in recent yearsIt has attracted widespread attention and effective exploration of social research institutions.The laboratory test of the trace pesticide residue (referred to as the agricultural residue) has been widely used through the method of launching by gas chromatography, gas chromatography-mass spectrometry (GC-MS method) and immune analysis technology.These traditional analysis technologies can meet t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 高大明江晓敏郑传阳许梦菡侯婷婷周杨群陈红朱德春
Owner HEFEI UNIV
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