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Carbon-gold nanocluster ratio fluorescent sensor microsphere as well as construction and application thereof

A gold nanocluster, ratio fluorescence technology, applied in the fields of materials science and engineering and environmental analytical chemistry, can solve problems such as uncontrollable, unfavorable fluorescent material application, complex construction process, etc., to achieve easy operation, rich applications, and simplified experimental steps Effect

Active Publication Date: 2018-06-29
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method usually involves processes such as chemical modification and chemical reaction, making the construction process complicated and uncontrollable
In addition, the cumbersome modification process may cause changes in the properties of the fluorescent material itself, which is not conducive to the application of fluorescent materials

Method used

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  • Carbon-gold nanocluster ratio fluorescent sensor microsphere as well as construction and application thereof
  • Carbon-gold nanocluster ratio fluorescent sensor microsphere as well as construction and application thereof
  • Carbon-gold nanocluster ratio fluorescent sensor microsphere as well as construction and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] For the process of ratiometric fluorescence sensor preparation, see figure 1 :

[0032] a. Preparation of carbon-gold nanoclusters: Weigh 46 mg of glutathione and add 2 mL of 50 mM chloroauric acid trihydrate solution, then add 48 mL of water, and reflux at 70° C. for 2 h to obtain a gold seed solution. Then weigh 10mg of glucose and dissolve in 5mL of the above-prepared gold seed solution, and then put it into a household microwave oven and microwave at medium heat (microwave power 700W, rated voltage 220V, rated frequency 50Hz) for 8min to prepare carbon-gold nanoclusters solution. Finally, it was dissolved in 5mL of water for use (see figure 2 A and Figure 6 ).

[0033] Depend on figure 2 A and Figure 6 It can be seen that the particle size of the obtained carbon-gold nanoclusters is between 2-5 nm, and the two emission peaks are at 466 nm and 598 nm, respectively.

[0034] b. Preparation of dithiothreitol-modified carbon-gold nanoclusters: Take 20 μL of D...

Embodiment 2

[0037] Take 10 μL of the carbon-gold nanoclusters and dithiothreitol-modified carbon-gold nanocluster solutions obtained in the above examples diluted 1000 times with ultrapure water, respectively, and disperse each dilution on the ethanol-washed copper On the net, after drying, the copper net loaded with the above-mentioned each diluted substance is observed with a transmission electron microscope (see figure 2 A-B); if figure 2 As shown in A, the particle size of carbon-gold nanoclusters is about 3nm, such as figure 2 As shown in B, the particle size of dithiothreitol-modified carbon-gold nanoclusters is also about 3nm, indicating that the modification of dithiothreitol will not cause the agglomeration or shape change of carbon-gold nanoclusters .

Embodiment 3

[0039] After the prepared dithiothreitol-modified carbon-gold nanocluster (DTT / C-AuNCs) solution was ultrasonically dispersed by an ultrasonic machine, three parallel copies were taken, and the same volume of 1mL DTT / C-AuNCs solution was added to a weighed In a heavy 1.5mL centrifuge tube, weigh again after drying. The concentration of the DTT / C-AuNCs solution was calculated as 0.50 mg / mL by calculating the ratio of the mass difference before and after the three parallel samples to the volume.

[0040] When measuring the fluorescence spectrum, take 0.2mL of the above-mentioned DTT / C-AuNCs solution with a concentration of 0.50mg / mL, and measure the total volume of the solution to be 0.5mL, that is, the concentration of DTT / C-AuNCs is 0.20mg / mL.

[0041] Prepare a series of PBS buffer solutions with pH values ​​of 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0. The specific method is to prepare sodium dihydrogen phosphate and disodium hydrogen phosphate solutions with a concent...

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Abstract

The invention belongs to the field of material science and engineering and environment analysis chemistry, and particularly discloses a dithiothreitol-modified carbon-gold nanocluster ratio fluorescent sensor microsphere and construction of the dithiothreitol-modified carbon-gold nanocluster ratio fluorescent sensor microsphere as well as application of the dithiothreitol-modified carbon-gold nanocluster ratio fluorescent sensor microsphere in high-selectivity high-sensitivity detection of mercury ions. A sensor utilizes a novel fluorescent nano-material carbon-gold nanocluster as a fluorescent source, and then thiol (-SH) is modified on the surface of the gold nanocluster through DTT (dithiothreitol); the mercury ions are attracted to the surface of the cluster by using the thiol; becauseaction force exists between the mercury ions (5d10) and gold ions (5d10), the high-selectivity high-sensitivity detection of the mercury ions can be realized. According to the carbon-gold nanoclusterratio fluorescent sensor microsphere as well as the construction and the application thereof disclosed by the invention, through a mixed solution of microwave glucose and gold seeds, the carbon-goldnanocluster with double emission wavelengths is prepared, so that a construction process of the ratio fluorescent sensor is simplified, and a complicated preparation process of a fluorescent compositematerial is effectively avoided. The carbon-gold nanocluster ratio fluorescent sensor microsphere is used for performing analysis and detection on the mercury ions in actual water samples, and a standard adding recovery ratio is 90 percent or above.

Description

technical field [0001] The invention belongs to the fields of material science and engineering and environmental analytical chemistry, and specifically relates to a dithiothreitol-modified carbon-gold nanocluster ratio fluorescent sensing microsphere and its construction and high selectivity and high sensitivity to mercury ions. Sensitive detection applications. Background technique [0002] As a typical heavy metal ion, mercury ion has biodegradability and bioaccumulation, which has caused serious threats to human health and ecological environment. The toxicity of mercury ions mainly comes from the strong attraction between it and the sulfhydryl groups of proteins and enzymes in cells, thus causing the dysfunction of the body. Therefore, it is necessary to develop a sensitive, rapid and efficient method for the detection of mercury ions. Traditional mercury ion analysis methods mainly include atomic absorption spectrometry, atomic fluorescence spectrophotometry, and induc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/64
CPCG01N21/6428G01N33/543G01N2021/6432
Inventor 李金花王晓艳陈令新刘雯
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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