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Molecular imprinting electrochemical sensor for selectively identifying chloramphenicol and fabrication method of molecular imprinting electrochemical sensor

A molecular imprinting and electrochemical technology, applied in the fields of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of cumbersome method of grafting initiators on the membrane surface, affecting the conductivity and sensitivity of sensors, conductivity or sensitivity problems, etc. Achieve the effect of shortened preparation time, high selective recognition ability, guaranteed conductivity and sensitivity

Inactive Publication Date: 2019-02-01
HUBEI INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modification of the functional membrane will also affect the conductivity and sensitivity of the sensor, and the grafting method of the membrane surface initiator is very cumbersome and complicated.
[0006] Therefore, the existing molecularly imprinted electrochemical sensors all have problems in conductivity or sensitivity

Method used

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  • Molecular imprinting electrochemical sensor for selectively identifying chloramphenicol and fabrication method of molecular imprinting electrochemical sensor
  • Molecular imprinting electrochemical sensor for selectively identifying chloramphenicol and fabrication method of molecular imprinting electrochemical sensor
  • Molecular imprinting electrochemical sensor for selectively identifying chloramphenicol and fabrication method of molecular imprinting electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of initiator graphene-hemin complex

[0037] Monolayer graphene oxide (0.83mmol, sheet diameter 50-200nm) and hemin (0.015mmol) were dissolved in 20ml of 50% ethanol solution, the pH was adjusted to 10.0 with 1mol / L sodium hydroxide solution, and ultrasound was performed for 30 minutes Finally, reflux at 100 degrees for 1 hour, cool and store in the dark;

[0038] (2) Preparation of molecularly imprinted electrochemical sensors

[0039] 2.1) Disperse the prepared graphene-hemin complex into dimethylformamide to make a 1 mg / mL solution, take 10 μL drop-coated on the surface of the glassy carbon electrode, and dry it under an infrared lamp.

[0040] 2.2) Dissolve functional monomer methacrylic acid (4 mmol) and template molecule chloramphenicol (1 mmol) in 2.5 ml acetonitrile, sonicate for 10 minutes, and incubate at room temperature for 1 hour;

[0041] 2.3) Insert the graphene-hematin modified electrode prepared in step 2.1) into the crosslinker N,N-me...

Embodiment 2

[0045] The molecularly imprinted electrochemical sensing electrode, the non-molecularly imprinted electrochemical sensing electrode and the bare glassy carbon electrode prepared in Example 1 were sequentially inserted into a three-electrode system consisting of a platinum counter electrode and a reference electrode in 10 ml 5 mmol L -1 K 3 Fe(CN) 6 In the aqueous solution, cyclic voltammetry scanning was carried out for 10 circles in the potential range of -0.3 ~ 0.6V, and the scanning speed was 50mV s -1 , to obtain the corresponding cyclic voltammetry curve. The result is as figure 2 As shown, after the surface of the glassy carbon electrode is modified with a molecularly imprinted polymer film, the current response becomes smaller, while the glassy carbon electrode modified with a non-molecularly imprinted polymer film has almost no current response. This is because the chloramphenicol molecularly imprinted film partially blocks the redox electrochemical behavior of the...

Embodiment 3

[0047] Insert the three-electrode system composed of the molecularly imprinted electrochemical sensing electrode, platinum counter electrode and reference electrode prepared in Example 1 into 10 ml of chloramphenicol containing 5 mmol L -1 K 3 Fe(CN) 6 In the aqueous solution, the concentration of chloramphenicol is 0-100 μg / L respectively. After magnetic stirring for 10 minutes, cyclic voltammetry scanning was carried out in the potential range of -0.3 to 0.6V for 10 cycles, and the scanning speed was 50mV s -1 , to obtain the mean value of the peak current response difference with the bare glassy carbon electrode, and to obtain the relationship curve between the peak current difference and the concentration of the chloramphenicol standard solution. The result is as image 3 As shown, in the concentration range of 0.5-100ng / mL, the peak current response maintains good linearity with the concentration of chloramphenicol, and the detection limit is 0.1ng / mL, which can realiz...

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Abstract

The invention discloses a molecular imprinting electrochemical sensor for selectively identifying chloramphenicol and a fabrication method of the molecular imprinting electrochemical sensor, and belongs to the field of a new material. The chloramphenicol is used as a template molecule, polymethacrylic acid is used as a functional monomer, N,N-methylene acrylamide is used as a crosslinking agent, agraphene-based composite metal nanometer material is used for triggering polymerization reaction, an electroinitiated free radical polymerization technology is combined, a molecular imprinting polymerization thin film is directly fabricated on a surface of an electrode, the chloramphenicol in a composite matrix can be rapidly identified, and high-sensitive quantitative detection is achieved. By the molecular imprinting electrochemical sensor, the dilemma problem of the molecular imprinting electrochemical sensor during the fabrication process are solved, direct film formation of a molecular imprinting material is achieved, and the conductivity of the molecular imprinting material is also ensured.

Description

technical field [0001] The invention relates to the field of new materials, in particular to a molecularly imprinted electrochemical sensor for selectively recognizing chloramphenicol and a preparation method thereof. Background technique [0002] In recent years, the problem of antibiotic abuse has widely existed in clinical practice and in the process of aquaculture and livestock and poultry breeding. With the process of cross-media transfer, the residual antibiotics are constantly migrating and transforming in the environmental medium. The combined form remains in animal tissues and eventually enters the human body, destroying the ecological balance of human gastrointestinal flora, causing rapid heartbeat, muscle tremors, nervous allergies, teratogenic, carcinogenic and mutagenic adverse reactions, resulting in antibiotic resistance and "clenbuterol" "Poisoning and other public health incidents. It not only poses a serious threat to human health, but also has a huge impa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/48
CPCG01N27/26G01N27/308G01N27/48
Inventor 郭少飞荆涛王鹏陈孝宇叶诚潘瑞花凌约涛陈诚
Owner HUBEI INSPECTION & QUARANTINE TECH CENT
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