Gold nanocluster preparation method and application thereof

A gold nanocluster and reaction technology, applied in the field of nanomaterials, can solve the problems of high price and limit the amount of DNA, and achieve the effects of good stability, high detection sensitivity and large Stokes shift.

Inactive Publication Date: 2013-08-28
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

DNA is also used in the synthesis of gold nanoclusters of different sizes, but its price is relatively

Method used

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  • Gold nanocluster preparation method and application thereof
  • Gold nanocluster preparation method and application thereof
  • Gold nanocluster preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, the polypeptide used is pentapeptide: CALNN(P1).

[0034] At 37°C, mix 3mL of P1 (5mg / mL) aqueous solution with 3mL of HAuCl 4 (4mM) was added to 10mL water successively, stirred for 5 minutes, then, slowly added 250μL 10mM freshly prepared NaBH 4 Carry out the reduction and continue the reaction for 3 hours, the color of the reaction solution will change from light yellow to light brown, successfully synthesizing gold nanoclusters with fluorescent effect.

[0035] The number of acidic amino acids and basic amino acids in P1 is the same, so there is no need to add NaOH to adjust the pH, and the reaction can also be realized in an acidic environment, and the pH of the solution after the reaction is 2.26.

[0036] Such as Figure 4 As shown, the synthesized gold nanoclusters have a size of 1.8±0.2nm and a maximum emission wavelength of 611nm (eg figure 1 As shown in the curve a), the solution is observed to have a strong fluorescence under the ultravio...

Embodiment 2

[0043] In this example, the polypeptide used is pentapeptide: DDCAGGEYDTFPYWDD(P2).

[0044] The reaction was carried out at 50°C, 0.0391g P2 was dissolved in 10mLNaOH (15mM) and ultrasonically stirred for 5 minutes, then 3.125mL HAuCl was added 4 (4mM), stirred for 5 minutes, and slowly added 250μL of 10mM freshly prepared NaBH 4 , and continue to react for 6 hours, the color of the reaction solution will change from light yellow to light brown, successfully synthesizing gold nanoclusters with fluorescent effect.

[0045]There are more acidic amino acid residues in P2 than basic amino acid residues, so it is necessary to add NaOH to adjust the pH to generate gold nanoclusters. By adjusting the reaction conditions, we found that under different reaction conditions, the synthesized gold nanoclusters have different wavelengths (such as Figure 12 , Figure 13 Shown), it was verified that the above reaction conditions were the optimal reaction conditions.

[0046] Such as F...

Embodiment 3

[0049] In this example, the polypeptide used is hexapeptide: CDDDDD(P3).

[0050] The reaction was carried out at 37°C, 5mL HAuCl 4 (4mM) and 5mL P3 (4mM) were mixed thoroughly, then, 2.5mL 50mM NaOH was added thereto, and after stirring for 5 minutes, 250μL 10mM NaBH freshly prepared was added thereto 4 , and continue to react for 4 hours, the color of the reaction solution will change from light yellow to light brown, successfully synthesizing gold nanoclusters with fluorescent effect.

[0051] There are more acidic amino acid residues in P3 than basic amino acid residues, so it is necessary to add NaOH to adjust the pH to form gold nanoclusters. By adjusting the reaction conditions, we found that under different reaction conditions, the synthesized gold nanoclusters have different wavelengths (such as Figure 14 , Figure 15 Shown), it was verified that the above reaction conditions were the optimal reaction conditions.

[0052] Such as Figure 6 As shown, the synthesi...

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Abstract

The invention provides a gold nanocluster preparation method and an application thereof, and belongs to the technical field of nano materials. The problem of high cost of gold nanoparticle compositing in the prior art is solved. The gold nanocluster preparation method includes the steps: a, mixing polypeptide water solution with HAuCl4 solution in a reaction vessel to prepare solution A; and b, adding NaBH4 solution into the solution A for reduction, and reacting for a certain time to obtain fluorescent gold nanocluster products. A series of gold nanoclusters with different maximum emission wavelengths are composited successfully by means of taking different polypeptides as templates and sodium borohydride as a reducer. The composited gold nanoclusters have the advantages of large Stokes shift, long fluorescence lifetime, good stability and the like, can be used for detecting mercury ions (Hg2+) in water samples, and is high in detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and relates to a preparation method and application of gold nanoclusters. Background technique [0002] Gold nanocluster is a new type of nanomaterial composed of several to dozens of gold atoms, and its size is comparable to the Fermi wavelength. Their ultra-small size determines that their optical and chemical properties are significantly different from those of larger gold nanoparticles. Because of the special electronic, catalytic, and optical properties of gold nanoclusters, as well as low toxicity, high chemical stability, and good biocompatibility, gold nanoclusters have been a research hotspot for scientists in recent years. Currently, templates successfully used to prepare gold nanoclusters include proteins, thiol compounds, DNA, and polymers. These gold nanoclusters have broad application space: detection, catalysis, biolabeling and bioimaging. [0003] Common biomolecule...

Claims

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Application Information

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IPC IPC(8): B82B3/00B82Y30/00G01N21/64
Inventor 黄宏周丹玲吕章英冯九菊王爱军
Owner ZHEJIANG NORMAL UNIVERSITY
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