Fluorescent probe for detecting viscosity and hydrogen peroxide and synthesis method and application of probe

A hydrogen peroxide and fluorescent molecular probe technology, applied in the field of chemical analysis and detection, can solve the problems of unfavorable biological sample detection, weak photobiological penetration ability, biological damage, unfavorable and other problems, and achieve high sensitivity, stable optical performance, and responsive fast effect
CN110172070AActive Publication Date: 2019-08-27SHANGQIU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGQIU NORMAL UNIVERSITY
Publication Date
2019-08-27

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Abstract

The invention discloses a fluorescent probe for detecting viscosity and hydrogen peroxide and a synthesis method and application of the probe, and belongs to the technical field of chemical analysis detection. The probe is obtained through a coupling reaction between aldehydic benzothiazole and a 4-methylpyridinium salt substituted by methylene phenylboronic acid. According to the structure of theprobe, due to the quenching effect of twisted intramolecular charge transfer (TICT), a probe molecule has almost no fluorescence emission characteristics. However, with the increase of the viscosityof an environmental system, the TICT effect is inhibited, and the probe molecule achieves strong fluorescence emission with the emission peak of 680 nm. In addition, the probe can also generate sensitive fluorescence response to H2O2, and the emission peak is 590 nm after the reaction. The concentration detection range of hydrogen peroxide is 0-25 Mumol L(-1), and the detection limit is 0.34 MumolL(-1). The probe can be used for detecting H2O2 in water and cells.
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Description

technical field

[0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a long-wavelength turn-on fluorescent probe for detecting viscosity and hydrogen peroxide and its combination method and application. Background technique

[0002] Hydrogen peroxide (H 2 o 2 ) is an important physiologically active oxygen molecule, and studies have shown that abnormal levels of hydrogen peroxide are associated with various diseases, such as inflammatory diseases, cardiovascular diseases, neurodegenerative diseases and cancer. On the other hand, viscosity is an important index parameter for the normal operation of cells, which can reflect the signal transduction of biomolecules in cells, the transportation of nutrients and metabolic waste. Changes in cell viscosity and abnormal levels of hydrogen peroxide have also been linked to a number of diseases. Therefore, the development of a simple and reliable method to realize the s...

Claims

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