Methods, compositions, and articles comprising stabilized gold nanoclusters

A technology of gold nanoclusters and compositions, which is applied in nanomedicine, nanostructure manufacturing, nanotechnology, etc., and can solve problems such as simplicity, sensitivity, and selectivity constraints

Inactive Publication Date: 2011-08-10
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many of these systems are constrained in simplicity, sensitivity, selectivity, and / or limited in practical application (e.g., incompatibility with aqueous environments)

Method used

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  • Methods, compositions, and articles comprising stabilized gold nanoclusters
  • Methods, compositions, and articles comprising stabilized gold nanoclusters
  • Methods, compositions, and articles comprising stabilized gold nanoclusters

Examples

Experimental program
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no. 1 approach

[0008] In some embodiments, the present invention provides methods. According to a first embodiment, the method for forming a plurality of gold nanoclusters comprises: forming a reaction mixture comprising a plurality of gold atom precursor molecules and a plurality of protein molecules, wherein the ratio of the gold atom precursor molecules to protein molecules is at least about 5:1; adjusting the pH of the reaction mixture to greater than about 11, and maintaining the reaction mixture at an appropriate temperature for a sufficient period of time to form a plurality of gold nanoclusters stabilized by at least one protein molecule, wherein the gold The average diameter of the nanoclusters is less than about 2 nm.

[0009] In another embodiment, a method of detecting mercury ions comprises: providing a composition comprising a plurality of gold nanoclusters and a protein or stabilizer, exposing the composition to a sample suspected of containing mercury ions, and determining wh...

Embodiment 1

[0073] The following examples describe the synthesis and properties of gold nanoclusters according to some embodiments of the invention.

[0074] Specifically, this example describes a simple one-pot "green" synthetic route based on the biomineralization capacity of a common commercially available protein, bovine serum albumin (BSA), for the preparation of with red emission (λ em Au-NCs with maximum = 640 nm, quantum yield (QY) ~ 6%). In this example, Au(III) ions were added to an aqueous solution of BSA. In some cases, BSA molecules chelate Au ions and trap them (see figure 1 ). The reducing ability of BSA molecules was activated by adjusting the pH of the reaction mixture to about 12; the trapped ions underwent stepwise reduction to form BSA-conjugated gold nanoclusters (BSA-Au-NCs) in situ. The as-prepared BSA-Au-NCs contained about 25 gold atoms (as evident from matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF) mass spectrometry) and were stable as...

Embodiment 2

[0086] The following examples describe additional experimental information on the gold nanoclusters made and used in Example 1.

[0087] All chemicals were purchased from Sigma-Aldrich and used as received. Use ultra-pure microporous water (18.2M).

[0088] Synthesis of red fluorescent Au-NCs. Aqua regia (HCl:HNO 3 volume ratio = 3:1) and rinsed with ethanol and ultrapure water. In a typical experiment, the HAuCl 4 Aqueous solution (5 mL, 10 mM, 37°C) was added to BSA solution (5 mL, 50 mg / mL, 37°C). After 2 minutes NaOH solution (0.5 mL, 1 M) was added and the reaction was allowed to proceed at 37 °C for 12 hours with vigorous stirring.

[0089] Material Characterization. Absorption and photoemission spectra were obtained with Agilent 8453 UV-visible spectrophotometer and Jobin Yvon Horiba Fluorolog spectrofluorometer, respectively. DLS analysis of BSA-Au-NC and BSA aqueous solutions was performed using a BI-200SM laser light scattering system (Brookhaven Instruments C...

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Abstract

The invention relates generally to gold nanoclusters, and in particular, fluorescent gold nanoclusters. The gold nanoclusters may be stabilized, for example, with a protein or stabilizing agent. In some cases, the gold nanoclusters may be used in methods or articles to determine the presence, absence, and / or concentration of mercuric ions in a sample.

Description

[0001] related application [0002] This application claims priority under 35 U.S.C. §119(e) to co-pending U.S. Provisional Application Serial No. 61 / 129,994, filed August 5, 2008, the contents of which are incorporated herein by reference. field of invention [0003] The present invention relates generally to gold nanoclusters, and in particular to fluorescent gold nanoclusters. The gold nanoclusters can be stabilized, for example, with proteins or stabilizers. In some cases, gold nanoclusters can be used in methods or articles of manufacture for determining the presence, absence and / or concentration of mercury ions in a sample. Background technique [0004] Noble metal nanoclusters (NCs) usually contain a few to tens of metal atoms and are usually smaller than 1 nm in size. The spatial confinement of free electrons in metal nanoclusters can generate discrete and size-tunable electronic transitions, leading to molecule-like properties such as luminescence and unique charg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64B82B1/00B82B3/00C09K11/58
CPCC09K11/58B82Y30/00B82Y15/00B82Y5/00G01N21/643G01N2021/7786C09K11/025B82B1/00B82B3/00G01N21/64
Inventor 应仪如谢建平郑远刚
Owner AGENCY FOR SCI TECH & RES
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