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Fluorescent ratio probebased on aggregation induced luminescence property and detection application to hydrogen peroxide and glucose of fluorescent ratio probe

A fluorescence ratio probe and aggregation-induced luminescence technology, which is applied in the intersection of chemistry and materials science, can solve the problems of self-quenching and easy interference of detection results, achieve broad application prospects, and realize the effect of visual semi-quantitative detection.

Active Publication Date: 2019-07-26
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a fluorescent ratiometric probe based on aggregation-induced luminescent properties and its hydrogen peroxide and glucose detection application in order to solve the problems of self-quenching and interference of detection results in the rapid detection of traditional fluorescent probes

Method used

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  • Fluorescent ratio probebased on aggregation induced luminescence property and detection application to hydrogen peroxide and glucose of fluorescent ratio probe
  • Fluorescent ratio probebased on aggregation induced luminescence property and detection application to hydrogen peroxide and glucose of fluorescent ratio probe
  • Fluorescent ratio probebased on aggregation induced luminescence property and detection application to hydrogen peroxide and glucose of fluorescent ratio probe

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Embodiment 1

[0035] A fluorescent ratiometric probe based on aggregation-induced luminescence properties, which is a composite material of tetraphenylethylene sulfonate and bovine serum albumin-coated gold nanoclusters. According to the different mixing ratios of the two, the color of the composite material appears red , yellow or blue;

[0036] A method for preparing a fluorescent ratio probe based on aggregation-induced luminescent properties, the specific steps are as follows:

[0037] Step 1. Prepare tetraphenylethylene sulfonate solution, weigh a certain amount of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (BSPOTPE) , dissolved in water with a concentration of 0.1mg / L.

[0038] Step 2: To prepare gold nanoclusters coated with bovine serum albumin, add 5mL of chloroauric acid (10mM) to 5mL of bovine serum albumin solution (50mM), adjust the pH to 10 with sodium hydroxide, and react at 37°C for 12 Hours, bovine serum albumin-coated gold nanoclusters (BSA-Au NC...

Embodiment 2

[0041] Detection of hydrogen peroxide: take 12.5 μL of bovine serum albumin-coated gold nanoclusters (5 mM, as Au in chloroauric acid 3+ Ion concentration meter) and 25 μL of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (0.1 mg / L) were mixed into a fluorescent ratiometric probe. 50 μL H 2 o 2 The solution (1, 2, 3, 4, 5, 6, 7, 8mM) was mixed with the above-mentioned fluorescent ratio probe, and 100 μL of PBS buffer (10 mM, pH 7) was added, and the volume was adjusted to 500 μL, and reacted at 37 ° C for 30 min, and then used The fluorometer scans the fluorescence emission wavelength of 410-850nm, and records the ratio of fluorescence intensity I and I 0 , I and I 0 The ratio (F 680 / F 490 ), to obtain the fluorescence ratio change value (I 0 -I) / I 0 The linear relationship between the concentration of hydrogen peroxide and C (I 0 -I) / I 0 =0.0322C+0.5347, such as image 3 shown. Using this linear relationship, hydrogen peroxide in unknown sampl...

Embodiment 3

[0043] Glucose detection: Take 12.5 μL bovine serum albumin-coated gold nanoclusters (5mM, as Au in chloroauric acid 3+ Ion concentration meter) and 25 μL of 1,2-bis[4-(3-sulfonic acid propoxy)phenyl]-1,2-stilbene sodium salt (0.1 mg / L) were mixed into a fluorescent ratiometric probe. Mix 100 μL glucose solution (1, 2, 3, 4, 5, 6, 7, 8, 9 mM) with 100 μL glucose oxidase (0.5 mg / L) solution, add 100 μL PBS buffer (10 mM, pH 7), add Add water to the fluorescence ratio probe, add water to make up to 500 μL, mix well, react at 37°C for 30 minutes, then scan the fluorescence emission wavelength of 410-850nm with a fluorometer, and record the fluorescence intensity ratio I and I 0 , I and I 0 The ratio (F 680 / F 490 ), to obtain the fluorescence ratio change value (I 0 -I) / I 0 Linear relationship with glucose concentration C (I 0 -I) / I 0 =0.0705C+0.0407, such as Figure 4 shown. Using this linear relationship, glucose in unknown samples can be quantified. Semi-quantitative...

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Abstract

The invention relates to a fluorescent ratio probebased on the aggregation induced luminescence property and detection application to hydrogen peroxide and glucose of thefluorescent ratio probe and belongs to the intersection field of chemistry and materials science. The fluorescent ratio probe comprises tetraphenylethylenesulfonate and gold nanoclusters coated with bovine serum albumin. The aggregation induced luminescence phenomenon is generated by the tetraphenylethylenesulfonatedue to the effect of electrostatic adsorption of the tetraphenylethylenesulfonate and the gold nanoclusters, a mixed solution presents dual emission wavelengths in410-550nm and in 500-850 nm correspondingly, and when the ratios of the tetraphenylethylenesulfonateto the gold nanoclustersare different, presented colors of a mixed fluorescent probe are different. Aggregation induced luminescence of the tetraphenylethylenesulfonate can be used as a reference fluorescence color, detection of the hydrogen peroxideand the glucose can be achieved according tothe linear change relationship betweenthe change value of the double emission peak fluorescence ratios (I<0>-I) / I<0> and analyte concentration C, and thefluorescent ratio probe has the advantages of being simple, fast and visual in the detection of the hydrogen peroxide and glucose.

Description

technical field [0001] The invention relates to a fluorescent ratio probe based on aggregation-induced luminescent properties and its application in the detection of hydrogen peroxide and glucose, relates to the preparation of nanomaterials and the detection of hydrogen peroxide and glucose, and belongs to the cross field of chemistry and material science. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is an important reactive oxygen species, widely used in industrial and household washing, bleaching and medical disinfection, but the high concentration of residual hydrogen peroxide in wastewater will cause serious and irreversible oxidative damage to aquatic organisms. On the other hand, hydrogen peroxide, as an important biological signal molecule, plays an important role in various biological processes. Therefore, hydrogen peroxide detection is crucial in both environmental protection and biomedical research fields. [0003] Glucose is an important substance...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/6428G01N21/78G01N2021/6432
Inventor 武晓丽薛健
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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