Rapid construction method of transcriptome sequencing library, and application of rapid construction method

A technology of transcriptome sequencing and construction method, which is applied in the field of rapid construction of transcriptome sequencing library, and achieves the effects of high library yield, efficient connection, and fast experimental process.

Active Publication Date: 2020-04-03
广州表观生物科技有限公司
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Problems solved by technology

However, there is no efficient library construction method for 100-300bp RNA

Method used

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  • Rapid construction method of transcriptome sequencing library, and application of rapid construction method
  • Rapid construction method of transcriptome sequencing library, and application of rapid construction method
  • Rapid construction method of transcriptome sequencing library, and application of rapid construction method

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Embodiment Construction

[0063] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any form.

[0064] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field. Unless otherwise specified, the reagents and materials used in the following examples are commercially available. Experimental methods that do not indicate specific conditions are usually implemented under conventional conditions or conditions suggested by the manufacturer.

[0065] In a specific embodiment of the present invention, combined with figure 1 As shown in the schematic flow chart of library construction, the present invention provides a rapid construction method of transcriptome sequencing library and its application.

[0066] The rapid construction method for transcriptome sequencing library ...

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Abstract

The invention provides a rapid construction method of a transcriptome sequencing library, and an application of the rapid construction method. Compared with the existing reports, the invention is an efficient library construction method specifically for RNA with a small and medium-length of 100-300 bp, and the construction method does not require any enzyme reaction and chemical reaction treatmentbefore synthesizing cDNA, and cannot cause degradation and loss of RNA during the treatment process. The experimental process is rapid and simple, the whole process has no complicated treatment of RNA and intermediate products, the construction method is especially suitable for the case of a small initial sample size, the efficient connection is realized, and finally the transcriptome library with a sufficiently high concentration is obtained to complete on-machine sequencing and subsequent transcriptome sequencing related applications. The widespread application of transcriptome sequencing of single cells, exosomes, or precious clinical samples in fields such as basic research, clinical diagnosis, and drug development is effectively realized.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on December 28, 2018, with the application number 201811620280.0, and the invention title is "A Method for Quickly Constructing a Transcriptome Sequencing Library and Its Application", the entire content of which is passed References are incorporated in this application. technical field [0002] The present invention relates to the field of high-throughput sequencing and library construction, and more specifically, to a method for rapidly constructing a transcriptome sequencing library and its application. Background technique [0003] The construction of cDNA library is the main technical means to study the functional genome of organisms. Different RNA-seq high-throughput sequencing library construction methods have different performances in terms of coverage, sensitivity, multiplexing ability, and the full-length rate of the built library. [0004] When const...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6869C40B50/06
CPCC12Q1/6806C12Q1/6869C40B50/06
Inventor 杨学敏陈永顺张燕菲高丰鑫
Owner 广州表观生物科技有限公司
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