Molecular marker for identifying amur sturgeon germplasm and application of molecular marker

Abstract

The invention provides a molecular marker for identifying amur sturgeon germplasm and a method for identifying amur sturgeon germplasm by virtue of the molecular marker. Amur sturgeon or a finished product thereof can be directly identified through sequence alignment on the basis of an interspecific sequence difference of an fzd8 gene segment obtained from PCR amplification of a specific primer between the amur sturgeon and other sturgeons. The molecular marker disclosed by the invention, which is used for analyzing and identifying an SNP site on the fzd8 nuclear gene segment screened from acipenseridae fishes, has the advantages of being accurate in experiment, reliable and rapid in identification, convenient and simple in operation, and the like.

Description

technical field

[0001] The invention relates to an identification method of fish germplasm, in particular to a molecular marker for identifying the germplasm of Acipenser's sturgeon and a method for identifying the germplasm of Acipenser's sturgeon by using the molecular marker. Background technique

[0002] Sturgeon fishes are one of the oldest surviving fishes on earth and are known as "living fossils". Acipenserdabryanus (Acipenserdabryanus) belongs to Acipenseridae, Acipenseridae, Acipensergenus, commonly known as seven floaters, etc. It is mainly distributed in the Heilongjiang water system, from the lower reaches to the upper reaches of the Ergun River and the Shilika River; most of them are distributed in the middle reaches of Heilongjiang and the In the lower reaches of the Songhua River, there are fewer Wusuli Rivers. Due to reasons such as overfishing and water pollution, the resources of Sturgeon's sturgeon have been drastically reduced. In 2010, Sturgeon's stur...

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