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Molecular marker for identifying amur sturgeon germplasm and application of molecular marker

A molecular marker, sturgeon technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. Effect

Active Publication Date: 2015-11-18
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patented method can only identify Acipenser schrencke to a certain extent, distinguishing Acipenser schrenckii from Hulus dauribus, and is only applicable to suspected samples known to be Acipenser shieldii or Acipenser dauribus, so the scope of application is narrow

Method used

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  • Molecular marker for identifying amur sturgeon germplasm and application of molecular marker
  • Molecular marker for identifying amur sturgeon germplasm and application of molecular marker
  • Molecular marker for identifying amur sturgeon germplasm and application of molecular marker

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Experimental program
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Effect test

preparation example Construction

[0034] The preparation method of 1% agar gel of the present invention is as follows:

[0035] Weigh 0.3g of agarose with a balance, pour it into 30ml of 1×TAE buffer, shake well, heat it in a microwave oven until completely dissolved, take it out and let it cool slightly (to prevent EB from volatilizing at high temperature), and add 2 μL of EB. Put the gel plate (Bio-Rad company) in a horizontal position, and then insert the sample comb. Pour the previously prepared agarose gel into the inner groove of the gel plate. Cool for half an hour and take out the comb after the gel is solidified, put the gel into an electrophoresis tank (Bio-Rad Company) and add 1×TAE electrophoresis buffer to submerge the gel. Spot 1 / 5 volume of spotting buffer (bromophenol blue) on the spotting plate.

Embodiment 1

[0037] The method for identifying the germplasm molecular markers of Acipenser schneideri, using Acipenser schnecke, Acipenser dabryi, Acipenser sinensis, Acipenser russiensis, Acipenser siberia, Acipenser sterletii and Acipenser dabryii as research objects, includes the following steps:

[0038] (I) Genomic DNA was extracted from the samples of Sturgeon's sturgeon, Dabry's sturgeon, Chinese sturgeon, Russian sturgeon, Siberian sturgeon, sterlet and Dauric sturgeon, using a marine tissue DNA extraction kit (Tiangen Company).

[0039] The template DNA was then detected by agarose gel electrophoresis:

[0040] Prepare 1% agarose gel, add 2 μL DNA solution to the sample buffer and mix well for electrophoresis. 100V voltage electrophoresis for 25min. After electrophoresis, determine the concentration of the template DNA according to the brightness of the electrophoresis band on the ultraviolet transmission detector, and dilute the template accordingly so that the final concentrat...

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Abstract

The invention provides a molecular marker for identifying amur sturgeon germplasm and a method for identifying amur sturgeon germplasm by virtue of the molecular marker. Amur sturgeon or a finished product thereof can be directly identified through sequence alignment on the basis of an interspecific sequence difference of an fzd8 gene segment obtained from PCR amplification of a specific primer between the amur sturgeon and other sturgeons. The molecular marker disclosed by the invention, which is used for analyzing and identifying an SNP site on the fzd8 nuclear gene segment screened from acipenseridae fishes, has the advantages of being accurate in experiment, reliable and rapid in identification, convenient and simple in operation, and the like.

Description

technical field [0001] The invention relates to an identification method of fish germplasm, in particular to a molecular marker for identifying the germplasm of Acipenser's sturgeon and a method for identifying the germplasm of Acipenser's sturgeon by using the molecular marker. Background technique [0002] Sturgeon fishes are one of the oldest surviving fishes on earth and are known as "living fossils". Acipenserdabryanus (Acipenserdabryanus) belongs to Acipenseridae, Acipenseridae, Acipensergenus, commonly known as seven floaters, etc. It is mainly distributed in the Heilongjiang water system, from the lower reaches to the upper reaches of the Ergun River and the Shilika River; most of them are distributed in the middle reaches of Heilongjiang and the In the lower reaches of the Songhua River, there are fewer Wusuli Rivers. Due to reasons such as overfishing and water pollution, the resources of Sturgeon's sturgeon have been drastically reduced. In 2010, Sturgeon's stur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李久煊杨坤刘东奇宋昭彬代伟荆慧芳陈永柏李翀刘勇
Owner CHINA THREE GORGES CORPORATION
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