Cell observation experimental method and device thereof

A cell and experiment technology, applied in the field of observation and experiment of cell research, can solve problems such as unsolved problems of automatic control of microfluidics, and achieve the effect of simple experiment

Inactive Publication Date: 2008-03-05
彭兴跃
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since it mainly involves experiments on cells in a microscopic environment, this patent does not solve the problem of how to realize the automatic control of microfluidics that affects cell observation experiments in practice, so there is a distance between theory and application

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  • Cell observation experimental method and device thereof
  • Cell observation experimental method and device thereof
  • Cell observation experimental method and device thereof

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Embodiment Construction

[0019] The present invention is an experimental method for cell observation, and the applicant has applied for a "PCT (PublicationNumber: WO / 2006 / 007701) for culturing and experimenting single-cell or multi-cellular granules in a chip" patent (the main content of this patent The content is that the selection, separation, positioning, retention and suspension culture of single cells in the microfluidic field of the chip are theoretically realized by using the microfluidic chip technology, and the cell particles can still be displayed continuously during the delivery and switching of reagents. Micro observation and recording), adding the following process: (1) The image capture device dynamically acquires the position, flow direction and speed data of the cells in the microchannel in the chip on the stage through the microscope and transmits them to the computer; (2) The computer real-time Collect and analyze the data collected by the image capture device, and calculate the relat...

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Abstract

Said apparatus comprises: a microscope, an image capturing device, a computer, a micro-pressure controller and a chip. In said chip, there are micro channels; said computer is connected to the port of image capturing unit through communication bus; the image capturing unit is connected to the microscope; the chip is placed on the object table; said micro-pressure controller is connected to each out-connection port of the chip micro channels via pneumatic pipeline; the micro-pressure controller is connected to the computer.

Description

technical field [0001] The invention relates to an observation experimental method for cell research, in particular to an experimental method for dynamic research on cells applied to scientific research and medical detection or teaching, and a device designed for realizing the method. Background technique [0002] In current biological or medical research and application, cell culture is the most important technology. However, as the basic unit of life, the method of isolating the basic unit for in vitro culture and experiment close to the living body has become a technical difficulty. The reason is that the microenvironment around the cells cannot be well controlled when the cells are cultured in vitro, but controlling the microenvironment is the basic method of scientific experiments and research. In particular, when researching cells, it is often necessary to introduce reagents and at the same time observe, record and track under the microscope, while the flowing liquid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84C12Q1/02
CPCB01L2300/0816B01L2200/146B01L2200/0647C12M41/46B01L2400/0487B01L3/50273C12M1/3446
Inventor 彭兴跃
Owner 彭兴跃
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