A strain of Pseudomonas aeruginosa and its isolation method and application
A Pseudomonas aeruginosa and liquid separation technology, applied in the environmental field, can solve problems that have not yet been discovered, and achieve the effect of increasing the degradation rate
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[0029] Enrichment and isolation of strain PAH-1
[0030] 1) Take 5mL anolyte from the sludge microbial fuel cell with a pH of 7 and inoculate it in 50mL liquid separation medium (the configuration of the sludge microbial fuel cell is as described in CN200910041235.4), the composition of the liquid separation medium is : Each liter of liquid separation medium contains 1.0g polycyclic aromatic hydrocarbons (electron donor), 16g 2,6-sodium anthraquinone disulfonate (ie AQDS, electron acceptor), 2.5g NaHCO 3 , 0.25g NH 4 Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution. Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, B6 10.0mg vitamin, 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin per liter of deionized water B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; the trace element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO per liter of deionized water...
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