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A strain of Pseudomonas aeruginosa and its isolation method and application

A Pseudomonas aeruginosa and liquid separation technology, applied in the environmental field, can solve problems that have not yet been discovered, and achieve the effect of increasing the degradation rate

Inactive Publication Date: 2011-12-28
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching the literature and patents, no report has been found on Pseudomonas aeruginosa that degrades PAHs under anaerobic conditions using humic substances as electron acceptors

Method used

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  • A strain of Pseudomonas aeruginosa and its isolation method and application
  • A strain of Pseudomonas aeruginosa and its isolation method and application
  • A strain of Pseudomonas aeruginosa and its isolation method and application

Examples

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Embodiment Construction

[0029] Enrichment and isolation of strain PAH-1

[0030] 1) Take 5mL anolyte from the sludge microbial fuel cell with a pH of 7 and inoculate it in 50mL liquid separation medium (the configuration of the sludge microbial fuel cell is as described in CN200910041235.4), the composition of the liquid separation medium is : Each liter of liquid separation medium contains 1.0g polycyclic aromatic hydrocarbons (electron donor), 16g 2,6-sodium anthraquinone disulfonate (ie AQDS, electron acceptor), 2.5g NaHCO 3 , 0.25g NH 4 Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution. Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, B6 10.0mg vitamin, 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin per liter of deionized water B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; the trace element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO per liter of deionized water...

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Abstract

The invention discloses a Pseudomonas aeruginosa strain PAH-1 capable of degrading PAHs, and its preservation number is CGMCC No.4773. Pseudomonas aeruginosa of the present invention can use humus as an electron acceptor under anaerobic conditions to oxidize and degrade polycyclic aromatic hydrocarbons, and can significantly improve the degradation rate of phenanthrene under glucose or fructose and phenanthrene co-metabolism conditions, and can be widely used in mild In situ bioremediation of polycyclic aromatic hydrocarbon contaminated soil.

Description

technical field [0001] The invention belongs to the field of environmental technology, in particular to a strain of Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), which exhibited efficient humus reduction and PAH degradation activities under anaerobic conditions. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of organic compounds with two or more benzene rings, including more than 150 compounds such as naphthalene, anthracene, phenanthrene, pyrene, etc., which have strong teratogenic, carcinogenic, Mutagenicity, with the characteristics of poor water solubility, strong stability, high toxicity, and difficult degradation, is a class of persistent organic pollutants that widely exist in the environment. As the main destination and important transfer station of polycyclic aromatic hydrocarbons in the environment, soil continuously releases polycyclic aromatic hydrocarbons to the surrounding environment through various physical and ch...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/385A62D101/20
Inventor 王跃强马晨周顺桂袁勇
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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