Pseudomonas aeruginosa for producing rhamnolipid biosurfactant with palm oil and application thereof

A technology of Pseudomonas aeruginosa and rhamnolipid, applied in the biological field, can solve the problems of medium waste, product loss, inability to achieve defoaming, etc., and achieve the effects of high fermentation yield, high conversion rate, and high fermentation yield

Active Publication Date: 2016-03-23
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional defoaming methods such as feeding defoaming agent, mechanical defoaming and pressurized defoaming cannot achieve the effect of defoaming, resulting in a large amount of foam carrying medium

Method used

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  • Pseudomonas aeruginosa for producing rhamnolipid biosurfactant with palm oil and application thereof
  • Pseudomonas aeruginosa for producing rhamnolipid biosurfactant with palm oil and application thereof
  • Pseudomonas aeruginosa for producing rhamnolipid biosurfactant with palm oil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Optimization of ammonium nitrate concentration in Pseudomonas aeruginosa TIBR02 inorganic salt fermentation medium: Pseudomonas aeruginosa TIBR02 was inoculated in 10% palm oil as carbon source, and the concentration of sodium nitrate was 5g / L, 7.5g / L, 10g In the inorganic salt medium of / L, 12.5g / L and 15g / L, the other components of the medium are: 1.1g / LKCl; 1.1g / LNaCl; 0.00028g / LFeSO4.7H2O; 3.4g / LKH2PO4; 4.4g / LK2HPO4; 0.5g / LMgSO4.7H2O; 0.5g / L yeast powder. The appropriate trace elements added to the inorganic salt medium include: 0.29g / LZnSO4.7H2O; 0.24g / LCaCl2.4H2O; 0.25g / LCuSO4.5H2O; 0.17g / LMnSO4.H2O. Fermentation uses 250ml shake flasks with a liquid volume of 25ml, cultured at 30°C and 200r / min for 144 hours, and samples are taken every 24h to detect rhamnolipid production, dry cell weight and residual palm oil. Pseudomonas aeruginosa TIBR02 has the highest yield and highest conversion rate when the concentration of sodium nitrate is 7.5g / L, and the...

Embodiment 2

[0026] Example 2 Optimization of palm oil concentration in inorganic salt fermentation medium of Pseudomonas aeruginosa TIBR02:

[0027] Pseudomonas aeruginosa TIBR02 was inoculated in inorganic salt medium with palm oil concentration of 75g / L, 100g / L, 125g / L and 150g / L respectively. The composition of inorganic salt medium was: 7.5g / LNaNO3; 1.1g / L LKCl; 1.1g / LNaCl; 0.00028g / LFeSO4.7H2O; 3.4g / LKH2PO4; 4.4g / LK2HPO4; 0.5g / LMgSO4.7H2O; 0.5g / L yeast powder. The appropriate trace elements added to the inorganic salt medium include: 0.29g / LZnSO4.7H2O; 0.24g / LCaCl2.4H2O; 0.25g / LCuSO4.5H2O; 0.17g / LMnSO4.H2O. A 250ml shake flask was used for fermentation with a liquid volume of 25ml, cultured at 30°C and 200r / min for 196 hours, and samples were taken every 24h to detect rhamnolipid production, dry cell weight and residual palm oil. The conversion rate of Pseudomonas aeruginosa TIBR02 was the highest when the concentration of palm oil was 75g / L, about 76.9%. Therefore, the concentra...

Embodiment 3

[0028] Example 3Under the optimal C / N conditions, palm oil was used as carbon source to carry out shake flask fermentation with Pseudomonas aeruginosa TIBR02: Pseudomonas aeruginosa TIBR02, 10% inoculum was inoculated in palm oil concentration of 75g / L, sodium nitrate 7.5g / L inorganic salt medium The other components of the medium are 1.1g / LKCl; 1.1g / LNaCl; 0.00028g / LFeSO4.7H2O; 3.4g / LKH2PO4; g / L yeast powder. The appropriate trace elements added to the inorganic salt medium include: 0.29g / LZnSO4.7H2O; 0.24g / LCaCl2.4H2O; 0.25g / LCuSO4.5H2O; 0.17g / LMnSO4.H2O. A 250ml shake flask was used for fermentation with a liquid volume of 25ml, cultured at 30°C and 200r / min for 216 hours, and samples were taken every 24h to detect rhamnolipid production, dry cell weight and residual palm oil. The highest yield was 57.73g / L in 192 hours, and the conversion rate was about 77%.

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Abstract

Rhamnolipid is an anionic surfactant which is free of toxicity and pollution, biodegradable, high in selectivity and specificity and excellent in surface activity and has unique application prospects in multiple industrial fields such as medicine, food, cosmetics and environment protection. The invention provides Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 with the high rhamnolipid yield and the high conversion rate through screening. Related fermentation equipment is transformed according to the fermentation characteristic of Pseudomonas aeruginosa, and a series connection fermentation experiment of 300L fermentation tanks is realized. According to the production state of the producing strain and the characteristic of the equipment, the production process of producing rhamnolipid in a fermented mode through Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 is developed, and while the rhamnolipid yield is high, the liquid leakage problem caused by surface activity brought by a great deal of rhamnolipid can be controlled. By using the equipment and the fermentation process, when palm oil with the concentration of 75 g/L serves as a carbon source, the rhamnolipid yield reaches 60.6 g/l, and the conversion rate reaches 80% or more. Therefore, the strain and the production process have good industrial application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Pseudomonas aeruginosa ( Pseudomonasaeruginosa ) TIB-R02CGMCCNo9376 and the method for producing rhamnolipid by fermentation of palm oil by the bacteria. Background technique [0002] Biosurfactant is a kind of non-toxic, biodegradable, non-polluting, good selectivity and specificity compared with chemically synthesized surfactants. Uniformity, good biocompatibility, etc., have unique application prospects in many industrial fields such as medicine, food, cosmetics, and environmental protection. Vigorously developing the biosurfactant technology industry will successfully drive the overall development of petroleum, medicine, detergent and other related fields, promote social progress and economic development, enable my country to have independent intellectual property rights, and improve international competitiveness. [0003] Rhamnosyl esters are anionic bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02C12R1/385
Inventor 王钦宏彭彦峰巩志金马延和
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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