Microbial agent for promoting growth of fruits and vegetables

Abstract

The invention relates to the technical field of agricultural microorganisms, and provides a microbial agent for promoting growth of fruits and vegetables, the microbial agent is mainly prepared from a compound bacteria liquid, the total viable count of the compound bacteria liquid is greater than or equal to 1 * 10 < 10 > CFU / g, and the compound bacteria liquid comprises the following components in percentage by viable bacteria: 35-40% of aspergillus terreus, 10-20% of aspergillus niger, 5-10% of aspergillus niger, 5-10% of aspergillus niger, and the balance of water. 10 to 20% of bacillus subtilis; 15 to 20% of streptomyces sp. 10-12% of saccharomyces cerevisiae; and 18 to 25% of pseudomonas chlororaphis. The invention overcomes the defects of the prior art, is reasonable in design and relatively comprehensive in function, and also solves the technical problems that the function of the existing single microbial agent is limited and the activity of the complex microbial agent is relatively low under a high-temperature condition.

Description

Technical Field

[0001] This invention relates to the field of agricultural microbiology, specifically to a microbial agent that promotes the growth of fruits and vegetables. Background Technology

[0002] Microbial inoculants, through their specific fertilizer effects, not only provide crops with essential nutrients, but their effective bacteria can also secrete active substances such as cytokinins and auxins. These active substances can stimulate, regulate, and promote crop growth and development, thereby contributing to increased crop yields.

[0003] Single microbial agents have limited functionality, and the yield increase per acre of compound microbial agents needs to be further improved. Therefore, we propose a microbial agent to promote the growth of fruits and vegetables. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the present invention provides a microbial agent for promoting the growth of fruits and vegetables, which overcomes the shortcomings of the prior art, has a reasonable design, and has more comprehensive functions. It also solves the technical problems of the functional limitations of existing single microbial agents and the low activity of compound microbial agents under high temperature conditions.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A microbial agent for promoting the growth of fruits and vegetables, characterized in that the microbial agent is mainly prepared from a compound bacterial solution, wherein the total viable count of the compound bacterial solution is ≥1×10⁻⁶. 10 CFU / g, expressed as a percentage of viable bacteria, includes: Aspergillus terreus 35-40%; Bacillus subtilis 10-20%; Streptomyces 15-20%; 10-12% brewer's yeast; Pseudomonas aeruginosa 18-25%.

[0006] Furthermore, the compound bacterial solution, calculated by percentage of viable bacteria, comprises: Aspergillus terreus 35%; Bacillus subtilis 15%; Streptomyces 20%; 10% brewer's yeast; 20% of Pseudomonas aeruginosa.

[0007] Furthermore, the microbial agent is prepared from a compound bacterial solution and an encapsulating agent, wherein the mass ratio of the compound bacterial solution and the encapsulating agent is 1:5 to 1:3.

[0008] Furthermore, the embedding agent is composed of the following raw materials by weight percentage: 12% maltodextrin; Soy protein isolate 8%; Calcium citrate 1.5%; Nanocellulose 0.5%; β-glucan 3%; Gum arabic 2%; The remainder is water.

[0009] Furthermore, the microbial inoculant is prepared by the following method: S1. Preparation of compound bacterial solution: After separately activating and fermenting Aspergillus terreus, Bacillus subtilis, Streptomyces, Saccharomyces cerevisiae, and Pseudomonas aeruginosa, the fermentation solutions of each bacterial solution are mixed in proportion. S2. Preparation of the embedding agent: Maltodextrin, soy protein isolate, nanocellulose, β-glucan, and gum arabic are added to water and stirred evenly to obtain the embedding agent premix. S3. Mixing of compound bacterial solution and embedding agent: Mix the compound bacterial solution and embedding agent premix according to the ratio, and add calcium citrate while stirring. S4. Drying: Obtain microbial inoculants by fluidized bed drying at 30℃.

[0010] Furthermore, the fruits and vegetables include tomatoes, watermelons, strawberries, lettuce, and corn.

[0011] This invention provides a microbial inoculant that promotes the growth of fruits and vegetables, and has the following beneficial effects: Aspergillus terreus: core functional bacteria, producing organic acids (phosphorus solubilizers), gibberellins to promote growth, and inhibiting nematodes; Bacillus subtilis: a resilient backbone that produces antimicrobial peptides; Streptomyces: Synergistic effect, secreting chitinase, activating Aspergillus terreus), producing siderophores to inhibit pathogens; Yeast: Metabolic activation, rapid fermentation produces small-molecule sugars and B vitamins. Pseudomonas aeruginosa: biocontrol function, producing phenazine antibiotics (antifungal diseases), siderophores (promoting root growth), and low temperature adaptability (still active at 4℃). The microbial agent obtained by compounding the above-mentioned multiple bacteria can not only effectively increase the yield per acre, make the fruit uniform and neat, and improve the sweetness of the fruit pulp, but also effectively overcome the problem of poor consistency in disease resistance and yield increase caused by the application of single bacteria (for example, the growth and bactericidal effect of Bacillus subtilis are significantly affected by a variety of microenvironmental factors, including soil temperature and humidity, pH value, crop growth status, etc. These factors lead to the instability of its growth process, and the effective bactericidal substances secreted are also easily degraded, making it difficult to maintain the consistency of its disease resistance and yield increase). This application, by compounding multiple bacteria and encapsulating them, can reduce the damage of external factors to the bacteria and achieve the slow release of bacteria, thus prolonging the effective action time. Detailed Implementation

[0012] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0013] Note on bacterial strains: The following bacterial strains are all commercially available products.

[0014] Aspergillus terreus AS 3.3935 was cultured on Czapek agar at a temperature of 28°C. Bacillus subtilis HF5 was cultured on nutrient broth agar at a temperature of 35-37℃. Streptomyces LJ3, cultured on PDA agar at 28°C; Saccharomyces cerevisiae HQA-8 was cultured on malt extract agar at a temperature of 25-28℃. Pseudomonas aeruginosa JD37 was cultured on 0002 nutrient broth agar at a temperature of 28-30℃.

[0015] Example 1

[0016] A microbial agent for promoting the growth of fruits and vegetables, wherein the microbial agent is prepared from a compound bacterial solution and an encapsulating agent, and the mass ratio of the compound bacterial solution and the encapsulating agent is 1:3; The compound bacterial solution, calculated by percentage of viable bacteria, includes: Aspergillus terreus 35%; Bacillus subtilis 15%; Streptomyces 20%; 10% brewer's yeast; Pseudomonas aeruginosa 20%; The embedding agent is composed of the following raw materials by weight percentage: 12% maltodextrin; Soy protein isolate 8%; Calcium citrate 1.5%; Nanocellulose 0.5%; β-glucan 3%; Gum arabic 2%; The remainder is water; The microbial inoculant is prepared by the following method: S1. Preparation of the compound bacterial solution: Aspergillus terreus, Bacillus subtilis, Streptomyces, Saccharomyces cerevisiae, and Pseudomonas aeruginosa were individually activated and fermented, and the concentration of the bacterial solution after fermentation of each strain reached 5 × 10⁻⁶. 9 CFU / g, each fermentation broth is filtered or centrifuged to concentrate it to one-fifth of its original volume, and then mixed in proportion to obtain the composite broth; S2. Preparation of the embedding agent: Maltodextrin, soy protein isolate, nanocellulose, β-glucan, and gum arabic are added to water, stirred, and mixed to obtain the embedding agent premix. S3. Mixing of compound bacterial solution and embedding agent: Mix the compound bacterial solution and embedding agent premix according to the ratio, stir, mix and emulsify, and add the remaining calcium citrate while stirring. S4. Drying: The product from step S3 is fed into a fluidized bed for granulation and dried at 30°C to obtain the microbial inoculant.

[0017] Application Example 1 The microbial inoculant prepared in Example 1 above was applied to tomato cultivation.

[0018] Tomato: Tianyuan No. 1.

[0019] Base fertilizer: 3000 kg / mu of farmyard manure, 100 kg / mu of microbial inoculant prepared in Example 1, 60 kg / mu of NPK compound fertilizer (NPK ratio of 15:15:15), 200 kg / mu of superphosphate, and 40 kg / mu of urea. Deeply plow into the soil to ensure that the fertilizer is fully mixed with the soil. Topdressing: From November of the previous year to February of this year, apply fertilizer once a month through fertigation, with each application consisting of 12 kg / mu of NPK compound fertilizer (NPK ratio of 15:15:15) and 2 kg / mu of urea. Simultaneously, apply fertilizer in holes once a month at a rate of 10 kg / mu. From March to May of this year, apply fertilizer every two weeks through fertigation, with each application consisting of 25 kg / mu of NPK compound fertilizer (NPK ratio of 15:15:15) and 8 kg / mu of urea. Simultaneously, apply fertilizer in holes once a month at a rate of 5 kg / mu.

[0020] Irrigation: Conventional irrigation.

[0021] Comparison 11: The composite bacterial solution in Example 1 above was replaced by a single Aspergillus terreus solution (with the same bacterial solution concentration), and the encapsulation agent and preparation method were the same; Control 12: The above-mentioned compound bacterial solution was replaced by a single Bacillus subtilis bacterial solution (with the same bacterial solution concentration), and the encapsulation agent and preparation method were the same; Control 13: The above compound bacterial solution was replaced by a single Aspergillus terreus solution (with the same bacterial solution concentration), omitting the embedding process, and obtained by spray drying or freeze drying to obtain bacterial powder; Control 14: The above compound bacterial solution was replaced by a single Bacillus subtilis bacterial solution (with the same bacterial solution concentration), omitting the encapsulation step, and obtained by spray drying or freeze drying to obtain bacterial powder. The crops were planted in five different experimental fields. The yield per mu of application example 1 fluctuated by 4.7%, while the yield per mu of control examples 11 and 12 fluctuated by 6.6% and 7.5%, respectively. The yield per mu of control examples 13 and 14 fluctuated by 8.6% and 10.2%, respectively.

[0022] Application Example 2 The microbial agent prepared in Example 1 was applied to watermelon plants.

[0023] Watermelon: Chuanmi 716.

[0024] Base fertilizer: 2000 kg / mu of farmyard manure, 500 kg / mu of human excrement, 200 kg / mu of cake fertilizer, and 100 kg / mu of microbial inoculant prepared in Example 1. Apply in furrows with a width of 50-60 cm and a depth of 25-40 cm. Then apply manure 15 days before transplanting, mix it with the bottom soil, and then fill the furrows. Fertilizer for seedling growth: Apply in holes, 8g of NPK compound fertilizer (NPK ratio of 15:15:15) and 1g of microbial agent prepared in Example 1 per plant; Vine-promoting fertilizer: Apply in holes, 50g of NPK compound fertilizer (NPK ratio of 15:15:15) and 1g of microbial agent prepared in Example 1 per plant; Fertilizer for fruit expansion: Apply in furrows, with 5 kg / mu of nitrogen fertilizer and 5 kg / mu of potassium fertilizer, and 2 kg / mu of microbial inoculant prepared in Example 1.

[0025] Topdressing: From November of the previous year to February of this year, apply fertilizer once a month through fertigation, with each application consisting of 12 kg / mu of NPK compound fertilizer (NPK ratio of 15:15:15) and 2 kg / mu of urea. Simultaneously, apply fertilizer in holes once a month at a rate of 10 kg / mu. From March to May of this year, apply fertilizer every two weeks through fertigation, with each application consisting of 25 kg / mu of NPK compound fertilizer (NPK ratio of 15:15:15) and 8 kg / mu of urea. Simultaneously, apply fertilizer in holes once a month at a rate of 5 kg / mu.

[0026] Comparison 21: The composite bacterial solution in Example 1 above was replaced by a single Aspergillus terreus solution (with the same bacterial solution concentration), and the encapsulation agent and preparation method were the same; Control 22: The above-mentioned compound bacterial solution was replaced by a single Bacillus subtilis bacterial solution (with the same bacterial solution concentration), and the encapsulation agent and preparation method were the same; Control 23: The above compound bacterial solution was replaced by a single Aspergillus terreus solution (with the same bacterial solution concentration), omitting the embedding process, and obtained by spray drying or freeze drying to obtain bacterial powder; Control 24: The above compound bacterial solution was replaced by a single Bacillus subtilis bacterial solution (with the same bacterial solution concentration), omitting the encapsulation step, and obtained by spray drying or freeze drying to obtain bacterial powder.

[0027] kilograms per mu Sweetness % Application Example 2 12560 14 Compare with 21 10840 13 Compare with 12 11368 13 Compare with 13 10267 13 Compare with 14 10985 12 The crops were planted in five different experimental fields. The yield per mu of application example 1 fluctuated by 6.3%, while the yield per mu of control examples 11 and 12 fluctuated by 8.9% and 10.1%, respectively. The yield per mu of control examples 13 and 14 fluctuated by 9.6% and 13.2%, respectively.

[0028] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0029] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microbial inoculant for promoting the growth of fruits and vegetables, characterized in that, The microbial agent is mainly prepared from a compound bacterial solution, and the total viable count of the compound bacterial solution is ≥1×10⁻⁶. 10 CFU / g, expressed as a percentage of viable bacteria, includes: Aspergillus terreus 35-40%; Bacillus subtilis 10-20%; Streptomyces 15-20%; 10-12% brewer's yeast; Pseudomonas aeruginosa 18-25%.

2. The microbial inoculant for promoting fruit and vegetable growth as described in claim 1, characterized in that: The compound bacterial solution, calculated by percentage of viable bacteria, includes: Aspergillus terreus 35%; Bacillus subtilis 15%; Streptomyces 20%; 10% brewer's yeast; 20% of Pseudomonas aeruginosa.

3. A microbial inoculant for promoting fruit and vegetable growth as described in claim 1 or 2, characterized in that: The microbial agent is prepared from a compound bacterial solution and an embedding agent, wherein the mass ratio of the compound bacterial solution and the embedding agent is 1:5 to 1:

3.

4. The microbial inoculant for promoting fruit and vegetable growth as described in claim 3, characterized in that: The embedding agent is composed of the following raw materials by weight percentage: 12% maltodextrin; Soy protein isolate 8%; Calcium citrate 1.5%; Nanocellulose 0.5%; β-glucan 3%; Gum arabic 2%; The remainder is water.

5. The microbial inoculant for promoting fruit and vegetable growth as described in claim 4, characterized in that: The microbial inoculant is prepared by the following method: S1. Preparation of compound bacterial solution: After separately activating and fermenting Aspergillus terreus, Bacillus subtilis, Streptomyces, Saccharomyces cerevisiae, and Pseudomonas aeruginosa, the fermentation solutions of each bacterial solution are mixed in proportion. S2. Preparation of the embedding agent: Maltodextrin, soy protein isolate, nanocellulose, β-glucan, and gum arabic are added to water and stirred evenly to obtain the embedding agent premix. S3. Mixing of compound bacterial solution and embedding agent: Mix the compound bacterial solution and embedding agent premix according to the ratio, and add calcium citrate while stirring. S4. Drying: Obtain microbial inoculants by fluidized bed drying at 30℃.

6. The microbial inoculant for promoting fruit and vegetable growth as described in claim 5, characterized in that: The fruits and vegetables mentioned include tomatoes, watermelons, strawberries, lettuce, and corn.