Amino acid water-soluble fertilizer as well as preparation method and application thereof

Abstract

The invention provides an amino acid water-soluble fertilizer as well as a preparation method and application thereof, and relates to the technical field of amino acid water-soluble fertilizers. The amino acid water-soluble fertilizer comprises compound amino acid, a microbial fermentation product, inorganic salt, a surfactant, a dispersing agent and a preservative, the microbial fermentation product is obtained by aerobic fermentation of radix puerariae and rhizoma atractylodis through a complex microbial inoculant; the compound amino acid is composed of animal protein hydrolyzed amino acid and vegetable protein hydrolyzed amino acid. According to the water-soluble fertilizer disclosed by the invention, through the synergistic interaction of the compound amino acid and the microbial fermentation product, multi-objective optimization of nutrition supply, stress resistance regulation and metabolism enhancement is realized, the yield and the quality of crops are remarkably improved, an efficient and environment-friendly solution for increasing the yield and improving the quality is provided for the crops, and the water-soluble fertilizer has a remarkable agricultural application value.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of amino acid water-soluble fertilizer, in particular to an amino acid water-soluble fertilizer, a preparation method and application thereof. BACKGROUND

[0002] In recent years, with the rapid development of modern agriculture, water-soluble fertilizer has been widely used in crop nutrition supplement and quality improvement due to its high efficiency, quick-acting and easy absorption. Amino acids, as important nutrients for plant growth, not only can directly participate in plant metabolism, but also can promote nutrient absorption and enhance stress resistance, so they have attracted much attention in the research and development of new fertilizers.

[0003] Although traditional chemical fertilizers have high nutrient content, long-term use can lead to soil compaction and environmental pollution. Ordinary organic fertilizers can improve soil, but have the disadvantages of slow effect and low absorption rate. Amino acid water-soluble fertilizer combines the environmental characteristics of organic fertilizer and the quick-acting property of chemical fertilizer, but the existing products still have problems such as single type of amino acid, insufficient stability, complex production process or high cost, which limits their popularization effect. In addition, some amino acid water-soluble fertilizers have limited functions and lack precise matching design for different crop growth needs, which affects their actual application effect.

[0004] Therefore, it is of great significance to develop an amino acid water-soluble fertilizer with scientific amino acid ratio, simple production process, high stability and both growth-promoting and stress-resistance functions, in order to meet the needs of green agriculture and improve crop yield and quality. SUMMARY

[0005] The present application aims to provide an amino acid water-soluble fertilizer, a preparation method and application thereof. The amino acid water-soluble fertilizer provided by the present application can significantly improve the yield and quality of crops.

[0006] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0007] The present application provides an amino acid water-soluble fertilizer, which comprises the following raw materials by weight: 20-30 parts of complex amino acid, 20-30 parts of microbial fermentation product, 10-20 parts of inorganic salt, 0.5-1.5 parts of surfactant, 1-3 parts of dispersant and 0.1-0.5 parts of preservative; the microbial fermentation product is obtained by aerobic fermentation of pueraria and atractylodes with a composite inoculant.

[0008] Preferably, the composite inoculant is composed of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1.

[0009] Preferably, the complex amino acid is composed of animal protein hydrolyzed amino acid and plant protein hydrolyzed amino acid, wherein the animal protein hydrolyzed amino acid is prepared by enzymolysis of defatted fish meal, and the plant protein hydrolyzed amino acid is prepared by enzymolysis of cottonseed meal.

[0010] Preferably, the inorganic salt is at least one of potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, and EDTA-Cu.

[0011] Preferably, the surfactant is at least one of dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether, the dispersant is at least one of polyacrylamide and sodium lignosulfonate, and the preservative is potassium sorbate.

[0012] The application further provides a preparation method of the amino acid water-soluble fertilizer, which comprises the following steps: mixing a compound amino acid, an inorganic salt, and water, and stirring under heating to obtain a mixed solution; and adding microbial fermentation products, a surfactant, a dispersant, and a preservative into the mixed solution, and stirring to obtain the amino acid water-soluble fertilizer.

[0013] The application further provides an application of the amino acid water-soluble fertilizer in promoting growth of crops, improving stress resistance, and increasing yield.

[0014] Preferably, the crops include grain crops, vegetable crops, and fruit tree crops.

[0015] Preferably, the amino acid water-soluble fertilizer is applied in a way of foliar spraying or flushing; the foliar spraying concentration is 0.5%-1.5% g / mL, and the flushing concentration is 0.2%-0.8% g / mL.

[0016] Compared with the prior art, the application has the following beneficial effects:

[0017] The application provides an amino acid water-soluble fertilizer, which comprises: a compound amino acid, microbial fermentation products, an inorganic salt, a surfactant, a dispersant, and a preservative; the microbial fermentation products are obtained by aerobic fermentation of kudzu root and atractylodes with a compound microbial agent; and the compound amino acid is composed of animal protein hydrolysis amino acid and plant protein hydrolysis amino acid. The compound amino acid prepared by the application can provide a comprehensive amino acid spectrum, enhance the osmotic regulation capacity of crops, and relieve drought, salinity, and other stresses; the microbial fermentation products contain various active ingredients, promote the absorption of crops, and have growth promotion and disease and pest resistance functions. The water-soluble fertilizer of the application realizes multi-target optimization of nutrient supply, stress resistance regulation, and metabolic enhancement through the synergistic effect of the compound amino acid and the microbial fermentation products, significantly improves the yield and quality of crops, provides an efficient and environmentally friendly yield-increasing and quality-improving solution for crops, and has significant agricultural application value. DETAILED DESCRIPTION

[0018] The application provides an amino acid water-soluble fertilizer, which comprises the following raw materials in parts by weight: 20-30 parts of complex amino acid, 20-30 parts of microbial fermentation product, 10-20 parts of inorganic salt, 0.5-1.5 parts of surfactant, 1-3 parts of dispersing agent and 0.1-0.5 parts of preservative; the microbial fermentation product is obtained by aerobic fermentation of pueraria and atractylodes by a composite microbial inoculant.

[0019] The composite microbial inoculant preferably consists of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1, wherein the Bacillus thuringiensis BRC-XQ7 is purchased from the China Agricultural Microbial Culture Collection Center, with the strain number ACCC 04358, the Aspergillus niger FSDZH-1 is purchased from the China Agricultural Microbial Culture Collection Center, with the strain number ACCC 32589, the number of Bacillus thuringiensis BRC-XQ7 in the composite microbial inoculant is preferably 200-500 million CFU / g, more preferably 300 million CFU / g, the number of Aspergillus niger FSDZH-1 in the composite microbial inoculant is preferably 300-800 million CFU / g, more preferably 500 million CFU / g, the inoculation amount of the composite microbial inoculant is preferably 1%-2% of the total weight of pueraria and atractylodes, more preferably 1.5%; the temperature of the aerobic fermentation is preferably 28-32 DEG C, more preferably 30 DEG C, the natural pH value is not additionally adjusted, the rotation speed is preferably 150-250 rpm, more preferably 200 rpm, and the time is preferably 3-5 days, more preferably 4 days.

[0020] Pueraria is rich in isoflavones such as puerarin and daidzin, and atractylodes contains atractylodes polysaccharide. After fermentation by Bacillus thuringiensis and Aspergillus niger, the proteins in the medicinal materials are decomposed into small molecule amino acids, and the polysaccharides are degraded into oligosaccharides, thereby improving the leaf surface absorption efficiency. At the same time, the fermentation product stimulates the crops to secrete defensive enzymes, activates the immune system and enhances the stress resistance. In addition, gibberellin GA3 (promoting cell elongation) and insecticidal proteins (such as Cry protein, preventing and treating lepidoptera pests) are generated during the fermentation process, further improving the growth and disease resistance of crops. It can be seen that the microbial fermentation product obtained by the preparation method of the application has the effects of providing high-activity plant-derived nutritional ingredients, improving stress resistance and promoting growth, thereby improving the yield, quality and stress resistance of crops.

[0021] The composite amino acid of the present application is preferably composed of animal protein hydrolyzed amino acid and plant protein hydrolyzed amino acid in a weight ratio of 1:2-4, more preferably 1:3. The animal protein hydrolyzed amino acid is prepared by enzymatic hydrolysis of defatted fish meal, and the plant protein hydrolyzed amino acid is prepared by enzymatic hydrolysis of cottonseed meal. The preparation of the animal protein hydrolyzed amino acid preferably comprises: mixing defatted fish meal with water, adding trypsin for enzymatic hydrolysis at 38-42℃ and pH 7.8-8.2 for 2-3h, inactivating the enzyme, adding flavor protease for enzymatic hydrolysis at 52-55℃ and pH 6.8-7.2 for 45-60min, filtering, and drying the filtrate to a water content of 2-5wt% to obtain the animal protein hydrolyzed amino acid. The preparation of the plant protein hydrolyzed amino acid preferably comprises: mixing cottonseed meal with water in a solid-liquid ratio of 1:20-25g / mL, adding papain and aminopeptidase for enzymatic hydrolysis at 48-52℃ and pH 7.2-7.8 for 2-3h, inactivating the enzyme, filtering, and drying the filtrate to a water content of 2-5wt% to obtain the animal protein hydrolyzed amino acid.

[0022] The enzymatic hydrolysates of animal protein (defatted fish meal) and plant protein (cottonseed meal) are complementary in amino acid composition. The fish meal hydrolysate is rich in neutral amino acids such as glycine and alanine, while the cottonseed meal hydrolysate contains more acidic amino acids such as glutamic acid and aspartic acid. The combination of the two can provide a more comprehensive amino acid spectrum to meet the nutritional needs of plants at different growth stages. The present application uses a double enzymatic hydrolysis process to decompose macromolecular proteins into small molecular active substances, which can promote the absorption of crops, improve the yield and quality of crops, and enhance the stability of crop cell membranes. Under adverse conditions such as drought, low temperature, and salt and alkali, it can regulate cell osmotic pressure, promote the accumulation of osmotic regulation substances such as proline, and alleviate stress damage.

[0023] The inorganic salt of the present application is preferably at least one of potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, and EDTA-Cu, more preferably the inorganic salt is composed of potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, and EDTA-Cu in a weight ratio of (50-60):(30-40):(20-30):(0.1-0.5):(0.01-0.05):(0.3-0.5):(0.1-0.3):(0.05-0.1):(0.02-0.05), more preferably 55:35:25:0.3:0.03:0.4:0.2:0.08:0.03. The inorganic salt can provide essential nutrients for plants, improve the growth, yield, and quality of crops by adjusting the nutrient ratio, improving solubility and bioavailability.

[0024] The surfactant is preferably at least one of dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether, more preferably consists of dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether at a weight ratio of 1-2:2-3, and more preferably 1.5:2.5. In use, dodecyl dimethyl betaine is mild and does not hurt leaves, is resistant to hard water, and enhances leaf spreading; lauryl alcohol polyoxyethylene ether has strong permeability, and can reduce interfacial tension and improve nutrient absorption efficiency when compounded with dodecyl dimethyl betaine, and the two compounds can reduce the dosage of a single agent while enhancing the rainwater washing resistance.

[0025] The dispersant is preferably at least one of polyacrylamide and sodium lignosulfonate, more preferably consists of polyacrylamide and sodium lignosulfonate at a weight ratio of 1:8-12, and more preferably 1:10. The selected dispersant can improve the suspension of the water-soluble fertilizer, prevent precipitation, and enhance stability.

[0026] The preservative is preferably potassium sorbate.

[0027] The application further provides a preparation method of the amino acid water-soluble fertilizer, which comprises: mixing the compound amino acid, the inorganic salt, and water, heating and stirring to obtain a mixed solution; adding the microbial fermentation product, the surfactant, the dispersant, and the preservative into the mixed solution, and stirring to obtain the amino acid water-soluble fertilizer.

[0028] The application further provides an application of the amino acid water-soluble fertilizer in promoting the growth of crops, improving the stress resistance, and increasing the yield, wherein the crops preferably include grain crops, vegetable crops, and fruit tree crops, and the amino acid water-soluble fertilizer is preferably applied in a way of foliar spraying or flushing; the foliar spraying concentration is preferably 0.5%-1.5% g / mL, and more preferably 1% g / mL, and the flushing concentration is preferably 0.2%-0.8% g / mL, and more preferably 0.5% g / mL.

[0029] The technical solutions in the application will be described clearly and completely in combination with the embodiments in the application. Obviously, the described embodiments are only some of the embodiments of the application, but not all the embodiments. Based on the embodiments in the application, all the other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.

[0030] In the following examples, if no special description is given, the methods are conventional methods.

[0031] In the following examples, if no special description is given, the materials and reagents can be obtained from commercial channels.

[0032] Bacillus thuringiensis BRC-XQ7 was purchased from China Agricultural Microorganism Culture Collection Center, with strain number ACCC04358; Aspergillus niger FSDZH-1 was purchased from China Agricultural Microorganism Culture Collection Center, with strain number ACCC 32589; Bacillus subtilis CICC 20522 was purchased from China Industrial Microorganism Culture Collection Center; Aspergillus oryzae CICC 40214 was purchased from China Industrial Microorganism Culture Collection Center; Defatted fish meal was purchased from Haixing Pasture Feed Co., Ltd., with item number 008; Cottonseed meal was purchased from Lingshou Xindeng Agricultural Products Co., Ltd., with item number XD-0087; Trypsin was purchased from Ningxia Xiasen Industry Group Co., Ltd., with enzyme activity of 200,000 U / g, and item number GDG-2009; Flavourzyme was purchased from Ningxia Xiasen Industry Group Co., Ltd., with enzyme activity of 50,000 U / g, and item number FFG-4710; Papain was purchased from Ningxia Xiasen Industry Group Co., Ltd., with enzyme activity of 100,000 U / g, and item number FDG-2203; Amino peptidase was purchased from Ningxia Xiasen Industry Group Co., Ltd., with enzyme activity of 5,000 U / g, and item number FDG-2251; Lauryl alcohol polyoxyethylene ether AEO-6 was purchased from Shandong Yousuo Chemical Technology Co., Ltd., with item number S12302125; Polyacrylamide was purchased from Guangzhou Lanquan Environmental Protection Technology Co., Ltd., with item number 2024003.

[0033] Example 1

[0034] (1) Preparation of microbial fermentation product

[0035] Pueraria and atractylodes were ground respectively, mixed according to a weight ratio of 2:1 to obtain a mixed powder, 20 times the weight of water was added to the mixed powder, sterilized, inoculated with a compound microbial inoculant, and fermented at 30°C and 200 rpm for 4 days. After sterilization, the filtrate was concentrated and dried to a water content of 5wt% to obtain a microbial fermentation product. The compound microbial inoculant was composed of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1. The number of Bacillus thuringiensis BRC-XQ7 in the compound microbial inoculant was 300 million CFU / g, the number of Aspergillus niger FSDZH-1 in the compound microbial inoculant was 500 million CFU / g, and the inoculation amount of the compound microbial inoculant was 1.5wt% of the mixed powder.

[0036] (2) Preparation of compound amino acid

[0037] Animal protein hydrolyzed amino acid: defatted fish meal was mixed with water according to a solid-liquid ratio of 1:15 g / mL, 3% of the weight of trypsin was added to the defatted fish meal, and the mixture was enzymatically hydrolyzed at 40°C and pH 8 for 2.5 h. After enzyme inactivation, 2% of the weight of flavourzyme was added to the defatted fish meal, and the mixture was enzymatically hydrolyzed at 53°C and pH 7 for 55 min. After enzyme inactivation, the filtrate was dried to a water content of 3wt% to obtain an animal protein hydrolyzed amino acid.

[0038] Plant protein hydrolyzed amino acid: cottonseed meal was mixed with water according to the solid-liquid ratio of 1:23 g / mL, 1% of the weight of the cottonseed meal was added with papain and 1.5% of the weight of the cottonseed meal was added with amino peptidase, and then the mixture was enzymolyzed at 50℃ and pH 7.5 for 2.5 h, the enzyme was inactivated, the mixture was filtered, and the filtrate was dried to a water content of 2 wt% to obtain the plant protein hydrolyzed amino acid;

[0039] The animal protein hydrolyzed amino acid and the plant protein hydrolyzed amino acid were mixed according to the weight ratio of 1:3 to obtain the compound amino acid;

[0040] (3) Preparation of the amino acid water-soluble fertilizer

[0041] Potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Cu were mixed according to the weight ratio of 50:40:25:0.3:0.03:0.4:0.2:0.08:0.03 to obtain the inorganic salt;

[0042] Dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether were mixed according to the weight ratio of 1.5:2.5 to obtain the surfactant;

[0043] Polyacrylamide and sodium lignosulfonate were mixed according to the weight ratio of 1:10 to obtain the dispersant;

[0044] The compound amino acid 25 parts, the microbial fermentation product 25 parts, the inorganic salt 15 parts, the surfactant 1 part, the dispersant 2 parts and potassium sorbate 0.3 part were weighed according to the weight parts;

[0045] The compound amino acid was mixed with deionized water according to the weight ratio of 1:4, and then the inorganic salt was added, and the mixture was stirred at 50℃ for 90 min to obtain the amino acid-inorganic salt complex, and then the microbial fermentation product, the surfactant, the dispersant and the potassium sorbate were added, and the mixture was stirred at 300 rpm for 40 min, and then the pH value was adjusted to 5.5 to obtain the amino acid water-soluble fertilizer.

[0046] Example 2

[0047] (1) Preparation of the microbial fermentation product

[0048] Kudzu root and Atractylodes lancea were pulverized separately and mixed in a 1:1 weight ratio to obtain a mixed powder. Water with 18 times the weight of the mixed powder was added, sterilized, and inoculated with a compound microbial agent. Fermentation was carried out at 28℃ and 150rpm for 5 days, sterilized, filtered, and the filtrate was concentrated and dried to a water content of 3wt% to obtain the microbial fermentation product. The compound microbial agent consisted of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1. The number of Bacillus thuringiensis BRC-XQ7 in the compound microbial agent was 200 million CFU / g, and the number of Aspergillus niger FSDZH-1 in the compound microbial agent was 300 million CFU / g. The inoculation amount of the compound microbial agent was 2wt% of the mixed powder.

[0049] (2) Preparation of complex amino acids

[0050] Hydrolyzed amino acids from animal protein: Defatted fish meal was mixed with water at a ratio of 1:12 g / mL. 2% (by weight) of trypsin from the defatted fish meal was added and hydrolyzed at 38℃ and pH 7.8 for 3 hours to inactivate the enzyme. 0.8% (by weight) of flavor protease from the defatted fish meal was added and hydrolyzed at 52℃ and pH 6.8 for 60 minutes to inactivate the enzyme. The mixture was then filtered, and the filtrate was dried to a water content of 4 wt% to obtain hydrolyzed amino acids from animal protein.

[0051] Hydrolyzed amino acids from plant protein: Cottonseed meal was mixed with water at a ratio of 1:20 g / mL, and 0.8% by weight of papain and 1% by weight of aminopeptidase from cottonseed meal were added. The mixture was enzymatically hydrolyzed at 48℃ and pH 7.2 for 3 hours, the enzymes were inactivated, the mixture was filtered, and the filtrate was dried to a water content of 4 wt% to obtain hydrolyzed amino acids from plant protein.

[0052] Animal protein hydrolysate amino acids and plant protein hydrolysate amino acids are mixed at a weight ratio of 1:2 to obtain a compound amino acid;

[0053] (3) Preparation of amino acid water-soluble fertilizer

[0054] Potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, and EDTA-Cu were mixed in a weight ratio of 60:30:20:0.5:0.01:0.5:0.1:0.1:0.02 to obtain an inorganic salt.

[0055] Dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether were mixed in a weight ratio of 1:2 to obtain a surfactant.

[0056] A dispersant was obtained by mixing polyacrylamide and sodium lignosulfonate at a weight ratio of 1:8.

[0057] Weigh out the following components by weight: 20 parts of compound amino acids, 20 parts of microbial fermentation product, 10 parts of inorganic salts, 0.5 parts of surfactant, 1 part of dispersant, and 0.1 parts of potassium sorbate.

[0058] The complex amino acid is mixed with deionized water at a weight ratio of 1:3, inorganic salt is added, and stirring is performed at 48°C for 100 min to obtain an amino acid-inorganic salt complex. Microbial fermentation product, surfactant, dispersant, and potassium sorbate are added, and stirring is performed at 250 rpm for 50 min. The pH value is adjusted to 5.3 to obtain an amino acid water-soluble fertilizer.

[0059] Example 3

[0060] (1) Preparation of microbial fermentation product

[0061] Pueraria and atractylodes are ground and mixed at a weight ratio of 3:1 to obtain a mixed powder. Water is added at 25 times the weight of the mixed powder, sterilized, inoculated with a complex microbial agent, and fermented at 32°C and 250 rpm for 3 days. The fermentation product is sterilized, filtered, concentrated, and dried to a water content of 3wt% to obtain a microbial fermentation product. The complex microbial agent is composed of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1. The number of Bacillus thuringiensis BRC-XQ7 in the complex microbial agent is 500 million CFU / g, the number of Aspergillus niger FSDZH-1 in the complex microbial agent is 800 million CFU / g, and the inoculation amount of the complex microbial agent is 1wt% of the mixed powder.

[0062] (2) Preparation of complex amino acid

[0063] Animal protein hydrolyzed amino acid: Defatted fish meal is mixed with water at a solid-liquid ratio of 1:18 g / mL. Trypsin is added at 3.5% of the weight of the defatted fish meal, and enzymatic hydrolysis is performed at 42°C and pH 8.2 for 2.5 h. The enzyme is inactivated, flavor protease is added at 2.5% of the weight of the defatted fish meal, and enzymatic hydrolysis is performed at 55°C and pH 7.2 for 45 min. The enzyme is inactivated, filtered, and the filtrate is dried to a water content of 4wt% to obtain animal protein hydrolyzed amino acid.

[0064] Plant protein hydrolyzed amino acid: Cottonseed meal is mixed with water at a solid-liquid ratio of 1:25 g / mL. Papain and aminopeptidase are added at 1.5% and 2% of the weight of the cottonseed meal, respectively, and enzymatic hydrolysis is performed at 52°C and pH 7.8 for 2 h. The enzyme is inactivated, filtered, and the filtrate is dried to a water content of 3wt% to obtain plant protein hydrolyzed amino acid.

[0065] The animal protein hydrolyzed amino acid and the plant protein hydrolyzed amino acid are mixed at a weight ratio of 1:4 to obtain a complex amino acid.

[0066] (3) Preparation of amino acid water-soluble fertilizer

[0067] Potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, EDTA-Cu are mixed according to the weight ratio of 55:45:30:0.1:0.05:0.3:0.3:0.05:0.05 to obtain inorganic salts;

[0068] Dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether are mixed according to the weight ratio of 1.5:2.5 to obtain a surfactant;

[0069] Polyacrylamide and sodium lignosulfonate are mixed according to the weight ratio of 1:10 to obtain a dispersant;

[0070] The composite amino acid 25 parts, the microbial fermentation product 25 parts, the inorganic salt 15 parts, the surfactant 1 part, the dispersant 2 parts and the potassium sorbate 0.3 part are weighed according to the weight parts respectively;

[0071] The composite amino acid is mixed with deionized water according to the weight ratio of 1:4, then the inorganic salt is added, and stirred at 50℃ for 90min to obtain an amino acid-inorganic salt complex, then the microbial fermentation product, the surfactant, the dispersant and the potassium sorbate are added, and stirred at 300rpm for 40min, and the pH value is adjusted to 5.5 to obtain an amino acid water-soluble fertilizer.

[0072] Comparative Example 1

[0073] The specific implementation is the same as that of Example 1, except that the "composite amino acid 25 parts, microbial fermentation product 25 parts" is replaced with "composite amino acid 50 parts", and the preparation of the microbial fermentation product in step (1) is deleted.

[0074] Comparative Example 2

[0075] The specific implementation is the same as that of Example 1, except that the "composite amino acid 25 parts, microbial fermentation product 25 parts" is replaced with "microbial fermentation product 50 parts", and the preparation of the composite amino acid in step (2) is deleted.

[0076] Comparative Example 3

[0077] The specific implementation is the same as that of Example 1, except that the "radix puerariae and atractylodes are respectively crushed, and mixed according to the weight ratio of 2:1 to obtain a mixed powder" in step (1) is replaced with "radix bupleuri and atractylodes are respectively crushed, and mixed according to the weight ratio of 2:1 to obtain a mixed powder".

[0078] Comparative Example 4

[0079] The specific implementation is the same as that of Example 1, except that step (1) is replaced by: the ginseng and atractylodes are respectively crushed, mixed according to a weight ratio of 2:1 to obtain a mixed powder, 20 times the weight of water is added to the mixed powder, sterilized, inoculated with a compound microbial inoculant, fermented at 37℃, 200rpm for 4d, sterilized, filtered, the filtrate is concentrated and dried to a water content of 5wt%, to obtain a microbial fermentation product; wherein the compound microbial inoculant is composed of Bacillus subtilis CICC 20522 and Aspergillus oryzae CICC 40214, the number of Bacillus subtilis CICC 20522 in the compound microbial inoculant is 300 million CFU / g, the number of Aspergillus oryzae CICC 40214 in the compound microbial inoculant is 500 million CFU / g, and the inoculation amount of the compound microbial inoculant is 1.5wt% of the mixed powder;

[0080] Comparative Example 5

[0081] The specific implementation is the same as that of Example 1, except that step (2) is replaced by: the defatted fish meal is mixed with water according to a solid-liquid ratio of 1:15g / mL, 3% of the weight of the defatted fish meal is added with trypsin for enzymolysis at 40℃, pH 8 for 2.5h, the enzyme is inactivated, 2% of the weight of the defatted fish meal is added with flavor protease for enzymolysis at 53℃, pH 7 for 55min, the enzyme is inactivated, filtered, and the filtrate is dried to a water content of 3wt% to obtain a compound amino acid.

[0082] Comparative Example 6

[0083] The specific implementation is the same as that of Example 1, except that step (2) is replaced by: the cottonseed meal is mixed with water according to a solid-liquid ratio of 1:23g / mL, 1% of the weight of the cottonseed meal is added with papain and 1.5% of the weight of the cottonseed meal is added with aminopeptidase for enzymolysis at 50℃, pH 7.5 for 2.5h, the enzyme is inactivated, filtered, and the filtrate is dried to a water content of 2wt% to obtain a compound amino acid.

[0084] Test Example 1

[0085] Tomato Field Experiment

[0086] Tomato Millennium is used as the experimental crop, and 8 groups (blank control group, experimental group (Example 1, Comparative Examples 1-6)) are set up, each group has 30m 2 , and each group has 100 plants. There is no significant difference in soil nutrient composition, light, temperature, humidity, etc. between the groups.

[0087] Each group of amino acid water-soluble fertilizer is diluted to 0.5%g / mL (flushing) and 1.0%g / mL (foliar spraying) with deionized water.

[0088] Spraying: the spraying dose is 100mL / m 2(i.e. the amount of diluted solution sprayed per square meter); spraying time, seedling stage (15 days after planting): first spray of ethanol to promote seedling growth; flowering and fruit setting stage (when the first inflorescence blooms): spray every 10 days for a total of 3 times; fruit enlargement stage: spray every 15 days for a total of 2 times.

[0089] Flush: the flush dose is 5L / m 2 (i.e. the amount of diluted solution flushed per square meter); flush time: growth stage (from planting to flowering), once every 15 days; fruiting stage, once every 10 days, staggered with foliar spraying by more than 3 days.

[0090] The blank control group is sprayed with an equal volume of water (100mL / m 2 ), and the flush is sprayed with an equal volume of water (5L / m 2 )

[0091] After the Qianxi matures, the yield of each group is counted, and the soluble solids and vitamin C content of the Qianxi fruit of each group are measured. The specific results are shown in Table 1.

[0092] The determination method of soluble solids is NY / T 2637-2014, and the determination method of vitamin C content is GB5009.86-2016.

[0093] Table 1: Yield, soluble solids and vitamin C content of tomatoes in each group

[0094]

[0095]

[0096] As can be seen from the data in Table 1, both the examples and the comparative examples can increase the yield, soluble solids and vitamin C content of Qianxi, and Example 1 has the greatest degree of increase. As can be seen from the data of Example 1 and Comparative Examples 1-2, the combination of complex amino acids and microbial fermentation products can increase the yield and quality of Qianxi. As can be seen from Example 1 and Comparative Examples 3-4, changes in fermentation substrate and fermentation microorganisms can change the composition of microbial fermentation products, thereby affecting the yield and quality of Qianxi. As can be seen from Example 1 and Comparative Examples 5-6, the addition of only animal-derived amino acids or plant-derived amino acids results in an incomplete range of amino acids, which can reduce the yield and quality of Qianxi.

[0097] Test Example 2

[0098] Cherry field experiment

[0099] 5-6 year old red cherry trees were used as experimental fruit trees, and 8 groups (blank control group, experimental group (Example 1, Comparative Examples 1-6)) were set up, with 10 red cherry trees in each group. There were no significant differences in soil nutrient composition, light, temperature, humidity, age of cherry trees, growth status, etc. between the groups.

[0100] Each group of amino acid water-soluble fertilizer was diluted with deionized water to 0.5% g / mL (drenching) and 1.0% g / mL (foliar spraying) respectively.

[0101] Spraying: the amount of each spraying was 120 mL / plant (i.e. the amount of diluted solution sprayed per square meter); the spraying time was as follows: the first spraying was performed at the germination stage (early March, when the bud was expanding), to promote flower bud differentiation; one spraying was performed before flowering (7 days before full bloom), to improve fruit setting rate; one spraying was performed at the young fruit stage (10 days after flowering), to promote fruit cell division; one spraying was performed at the fruit enlargement stage (30 days after flowering), to improve sugar content.

[0102] Drenching: the amount of drenching was 12 L / plant (i.e. the amount of diluted solution drenched per square meter); the drenching frequency was as follows: one drenching was performed before flowering, to promote root activity; one drenching was performed at the hard core stage (20 days after flowering), to reduce fruit drop; one drenching was performed after fruit picking, to restore tree vigor.

[0103] The blank control group was sprayed with an equal volume of water (120 mL / plant) and drenched with an equal volume of water (12 L / plant).

[0104] After the red light cherries were ripe, they were picked, and the yield of each group was counted, and the soluble solids and anthocyanin content of the cherries in each group were determined, and the specific results are shown in Table 2.

[0105] The determination method of soluble solids was NY / T 2637-2014, and the determination method of anthocyanin content referred to NY / T2640-2014.

[0106] Table 2 Yield, soluble solids and anthocyanin content of red light cherries in each group

[0107] Group Yield (kg / plant) Soluble solids (%) Anthocyanins (mg / g) Blank control 16.28 16.34 92.8 Example 1 24.23 23.66 188.1 Comparative Example 1 18.81 19.81 136.7 Comparative Example 2 19.66 19.58 143.0 Comparative Example 3 20.19 20.92 154.9 Comparative Example 4 21.82 21.49 161.2 Comparative Example 5 20.87 21.75 157.5 Comparative Example 6 21.04 22.07 149.3

[0108] As can be seen from the data in Table 2, the yield, soluble solids and anthocyanin content of cherries in the examples and comparative examples were all improved compared with the blank control group. As can be seen from the data in Example 1 and Comparative Examples 1-2, the compound amino acid and the microbial fermentation product had a synergistic effect, which could significantly improve the yield and quality of cherries. As can be seen from Example 1 and Comparative Examples 2-6, changing the fermentation substrate, changing the fermentation microorganism, adding only animal-derived amino acids or plant-derived amino acids would all affect the yield and quality of cherries.

[0109] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the protection scope of the present application.

Claims

1. An amino acid water-soluble fertilizer, characterized in that, The raw materials include the following parts by weight: 20-30 parts of compound amino acids, 20-30 parts of microbial fermentation product, 10-20 parts of inorganic salts, 0.5-1.5 parts of surfactant, 1-3 parts of dispersant, and 0.1-0.5 parts of preservative; The microbial fermentation product is obtained by aerobic fermentation of kudzu root and atractylodes lancea with a compound bacterial agent.

2. The amino acid water-soluble fertilizer according to claim 1, characterized in that, The compound microbial agent is composed of Bacillus thuringiensis BRC-XQ7 and Aspergillus niger FSDZH-1.

3. The amino acid water-soluble fertilizer according to claim 2, characterized in that, The compound amino acid is composed of hydrolyzed amino acids from animal protein and hydrolyzed amino acids from plant protein. The hydrolyzed amino acids from animal protein are obtained by enzymatic hydrolysis of defatted fish meal, and the hydrolyzed amino acids from plant protein are obtained by enzymatic hydrolysis of cottonseed meal.

4. The amino acid water-soluble fertilizer according to claim 1, characterized in that, The inorganic salt is at least one of potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, boric acid, sodium molybdate, EDTA-Fe, EDTA-Mn, EDTA-Zn, and EDTA-Cu.

5. The amino acid water-soluble fertilizer according to claim 1, characterized in that, The surfactant is at least one of dodecyl dimethyl betaine and lauryl alcohol polyoxyethylene ether.

6. The amino acid water-soluble fertilizer according to claim 1, characterized in that, The dispersant is at least one of polyacrylamide and sodium lignosulfonate, and the preservative is potassium sorbate.

7. A method for preparing an amino acid water-soluble fertilizer according to any one of claims 1-6, characterized in that, include: The compound amino acids, inorganic salts and water are mixed and heated and stirred to obtain a mixture; microbial fermentation products, surfactants, dispersants and preservatives are added to the mixture and stirred to obtain an amino acid water-soluble fertilizer.

8. The application of an amino acid water-soluble fertilizer according to any one of claims 1-6 in promoting crop growth, improving stress resistance and increasing yield.

9. The application according to claim 8, characterized in that, The crops mentioned include food crops, vegetable crops, and fruit trees.

10. The application according to claim 8, characterized in that, The amino acid water-soluble fertilizer is applied by foliar spraying or fertigation; the concentration for foliar spraying is 0.5%-1.5% g / mL, and the concentration for fertigation is 0.2%-0.8% g / mL.

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