Extraction and application of exopolysaccharide metabolite of bacillus amyloliquefaciens strain

A technology for dissolving starch spores and exopolysaccharides, which can be applied to bacteria, microorganisms, microorganism-based methods, etc., and can solve problems such as unreported research.

Inactive Publication Date: 2012-12-12
XI AN JIAOTONG UNIV
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Problems solved by technology

[0008] It has been reported that Bacillus amyloliquefaciens has bacteriostatic properties (including fungi and bacteria), an

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  • Extraction and application of exopolysaccharide metabolite of bacillus amyloliquefaciens strain
  • Extraction and application of exopolysaccharide metabolite of bacillus amyloliquefaciens strain
  • Extraction and application of exopolysaccharide metabolite of bacillus amyloliquefaciens strain

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[0038] The present invention will be described in detail below in conjunction with examples and accompanying drawings.

[0039] Isolation source: The no-clean vegetable assortment sample collected from a supermarket, homogenized in a sterile state, weighed 0.5 sample, added sterilized 0.1% peptone solution to the test tube, mixed well, stood at room temperature for 5 minutes, and took 1ml of the supernatant Mix with 25ml of sterilized melted beef extract-peptone solid medium, pour the plate, and incubate at 37°C for 48h. It was found that the bacterium grew rapidly on beef extract-peptone solid medium, and the colonies were smooth, moist, and viscous when touched. When cultured in LB liquid test tube medium, cell flocculation appeared after 48 hours.

[0040]Medium for separation: beef extract-peptone solid medium (0.3% beef extract, 1% peptone, 0.5% sodium chloride, 1.5% agar, autoclaved, 121°C, 20min)

[0041] The exopolysaccharide metabolites of a Bacillus amyloliquefacien...

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Abstract

The invention discloses an extraction and an application of exopolysaccharide metabolite of a bacillus amyloliquefaciens C-1 strain. A 16s rRNA amplified fragment of the bacillus amyloliquefaciens C-1 strain is sequenced and compared with a 16s rRNA sequence of bacteria in a NCBI (national center of biotechnology information) database; a comparison result proves that the bacillus amyloliquefaciens C-1 strain belongs to B.amyloliquefaciens; and an NCBI register number of the 16s rRNA sequence of the bacillus amyloliquefaciens C-1 strain is JN974457. The bacillus amyloliquefaciens C-1 strain is collected in china center for type culture collection (CCTCC), has the effects of reducing exopolysaccharide, removing DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, scavenging hydroxyl free radical (.OH), scavenging superoxide anion (O<2->), inhibiting lipid peroxidation and inhibiting human tumor cell proliferation, and opens up a new way and a new research field for exploring anti-tumor drugs.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the extraction and application of extracellular polysaccharide metabolites of a Bacillus amyloliquefaciens C-1 strain. technical background [0002] In recent years, the use of microorganisms and their antibacterial substances to prevent and control fungal diseases of crops has become one of the important research directions. Caldelra et al. isolated a strain of Bacillus amyloliquefaciens CCMI 1051 (Bacillus amyloliquefaciens) against Rhizopus L-122 (Rhizopussp). and Trichoderma harzianum CCMI 783 (Trichoderma harzianum) have a strong inhibitory effect [Annals of micrbilology, 2007,57:29-33]. Arrebola et al. reported that Bacillus amyloliquefaciens PPCB004 can inhibit the mycelium extension of Penicillium crustosum Thom and other fungi of the genus Penicillium. Lee et al. found that Paenibacillus lentimorbus strain WJ5 (Paenibacillus lentimorbus) inhibited the pathogenici...

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/07
Inventor 罗文娟韩蓓李旭刘翠翠韩蓁罗国刚张彦民
Owner XI AN JIAOTONG UNIV
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