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Method for increasing antioxidation activity of flammulina velutipes polysaccharide

A technology of antioxidant activity and Flammulina velutipes polysaccharides, which is applied in the field of Flammulina velutipes deep processing, can solve the problems of low yield of Flammulina velutipes polysaccharides, cumbersome operations, complicated procedures, etc., achieve obvious antioxidant activity, reduce molecular weight, and have a wide range of applications

Active Publication Date: 2013-05-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Flammulina velutipes polysaccharide prepared by the above method not only has low yield and low activity, but also has complex procedures, cumbersome and uncontrollable operations.

Method used

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  • Method for increasing antioxidation activity of flammulina velutipes polysaccharide
  • Method for increasing antioxidation activity of flammulina velutipes polysaccharide
  • Method for increasing antioxidation activity of flammulina velutipes polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Wash the Flammulina velutipes, dry them and crush them to 60 meshes to obtain raw materials.

[0032] (2) Add 3 times 95% ethanol to the dried Flammulina velutipes, heat to make the liquid slightly boil, and reflux for 2 hours. Remove impurities, filter, remove alcohol, and take the filter residue to obtain Flammulina velutipes powder.

[0033] (3) Add 20 times of distilled water to the extraction material, extract at 90°C for 1 hour, filter, repeat the extraction once, and separate the residue of Flammulina velutipes to obtain the extract.

[0034] (4) Add 3 times Sevag reagent (chloroform:butanol=5:1) to the extract to remove protein, shake vigorously for 30 minutes, then let stand for 10 minutes, centrifuge, and repeat protein removal 3 times.

[0035] (5) Vacuum-concentrate the extract to 1 / 7 of its original volume to obtain a concentrate. Then add ethanol so that the final concentration of ethanol is 80% (v / v), mix well, let stand at 0-4°C for 6 hours, filter...

Embodiment 2

[0039] (1) Wash the Flammulina velutipes, dry them and crush them to 80 meshes to obtain raw materials.

[0040] (2) Add 5 times 95% ethanol to the dried Flammulina velutipes, heat to make the liquid slightly boil, and reflux for 3 hours. Remove impurities, filter, remove alcohol, and take the filter residue to obtain Flammulina velutipes powder.

[0041] (3) Add 25 times of distilled water to the extracted raw materials, extract at 95°C for 2 hours, filter, repeat the extraction once, and separate the residue of Flammulina velutipes to obtain the extract.

[0042] (4) Add 4 times Sevag reagent (chloroform:butanol=5:1) to the extract to remove protein, shake vigorously for 45 minutes, then let stand for 10 minutes, centrifuge, and repeat protein removal 3 times.

[0043] (5) Vacuum-concentrate the extract to 1 / 5 of the original volume to obtain a concentrate. Then add ethanol so that the final concentration of ethanol is 75% (v / v), mix well, let stand at 0-4°C for 10 hours, ...

Embodiment 3

[0047] (1) Wash the Flammulina velutipes, dry them and crush them to 60 meshes to obtain raw materials.

[0048] (2) Add 6 times of 95% ethanol to the dried Flammulina velutipes, heat to make the liquid slightly boil, and reflux for 4 hours. Remove impurities, filter, remove alcohol, and take the filter residue to obtain Flammulina velutipes powder.

[0049] (3) Add 30 times of distilled water to the extracted raw materials, extract at 100°C for 3 hours, filter, repeat the extraction once, and separate the residue of Flammulina velutipes to obtain the extract.

[0050] (4) Add 5 times Sevag reagent (chloroform:butanol=5:1) to the extract to remove protein, shake vigorously for 60 minutes, then let stand for 10 minutes, centrifuge, and repeat protein removal 3 times.

[0051] (5) Vacuum-concentrate the extract to 1 / 3 of the original volume to obtain a concentrate. Then add ethanol so that the final concentration of ethanol is 85% (v / v), mix well, let stand at 0-4°C for 12 hours...

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Abstract

The present invention discloses a preparation method for increasing antioxidation activity of flammulina velutipes polysaccharide, wherein an enzymolysis control method is adopted to regulate and control molecular weight distribution of flammulina velutipes polysaccharide, flammulina velutipes is sequentially subjected to crushing, impurity removing, hot water extraction, protein removing, concentration and ethanol fractional precipitation separation to obtain the flammulina velutipes polysaccharide, and an enzyme method is adopted to carry out degradation control so as to reduce a molecular weight of the flammulina velutipes polysaccharide to 19000-30000 Da. The flammulina velutipes polysaccharide prepared by using the method is suitable for industrial production, wherein a polysaccharide extraction rate is increased by 2.5-3.5 times compared with a polysaccharide extraction rate of the traditional water extraction method, significant antioxidation activity is provided, IC50 of the flammulina velutipes polysaccharide in a DPPH.radical system is 1.69-2.12 mg / mL, and an ORAC value can be up to 923 mumol Trolox / g.

Description

technical field [0001] The invention relates to the deep processing technology of Flammulina velutipes, in particular to a method for improving the antioxidant activity of Flammulina velutipes polysaccharides. Background technique [0002] Flammulina velutipes is a famous edible fungus, medicinal fungus and ornamental fungus in the world. It is second only to mushrooms and shiitake mushrooms, and belongs to the third edible fungus. At present, Flammulina velutipes has been widely used in food, condiment and health care products industries. Polysaccharides are important biologically active substances in Flammulina velutipes. Relevant studies have shown that the polysaccharides of Flammulina velutipes are a type of glucan mainly connected by β-(1, 3) glycosidic bonds, which have the functions of inhibiting tumors, anticancer, lowering blood sugar, lowering blood lipids and enhancing The body's immunity and so on. At present, Flammulina velutipes polysaccharides have been dev...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 游丽君赵谋明崔春刘钧发冯梦莹任娇艳
Owner SOUTH CHINA UNIV OF TECH
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