A kind of extraction method of fungus polysaccharide

A technology of ground fungus and polysaccharides, which is applied in the field of extraction of ground fungus polysaccharides, can solve the problems of large loss of raw materials, complicated preparation process, and low extraction rate of polysaccharides, and achieve reduced raw material costs, simple operation methods, high extraction rates and recovery rates Effect

Active Publication Date: 2018-10-26
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] It can be seen from the above scheme that the preparation method needs to go through more operation steps, the preparation process is more complicated, the loss of raw materials is more, and the extraction rate of polysaccharides is low.

Method used

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  • A kind of extraction method of fungus polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for extracting polysaccharides from fungus, such as figure 1 shown, including the following steps:

[0053] (1) Preparation of ground fungus powder suspension: 100 g of ground fungus powder was dissolved in [Bmim]Cl ionic liquid with a concentration of 0.4 mol / L, and extracted for 30 minutes to prepare ground fungus powder suspension.

[0054] (2) Preparation of ground fungus cell crushing solution: use ultrasonic-microwave combined reaction system to crush the ground fungus powder cells in the prepared ground fungus powder suspension, set the ultrasonic power to 150w, ultrasonic treatment time 8min; microwave power to 160w , microwave heating treatment time 3min, during microwave heating treatment, adopt intermittent treatment mode, heating 1min, intermittent 1min, centrifugation treatment 15min with the speed of 3500rpm afterwards, take supernatant, obtain the ground fungus cell broken liquid.

[0055] (3) Preparation of crude extract of Auricularia auricula...

Embodiment 2

[0062] Weigh 100g of ground fungus powder, add 15 times the amount of 0.3mol / L ionic liquid, and extract at room temperature for 30 minutes; at 100w ultrasonic power, 5min ultrasonic time, 150w microwave power, microwave heating adopts intermittent treatment, heating 1min, intermittent 1min, microwave Treat for 3 minutes, centrifuge at 3000 rpm for 15 minutes to obtain supernatant;

[0063] Add ammonium sulfate to the supernatant to make the saturation 70%, let stand at 4°C for 2 hours, centrifuge at 5000 rpm for 10 minutes, and take the supernatant to obtain the crude extract of Auricularia polysaccharide;

[0064] The crude polysaccharide extract and the reverse micelles solution were fully mixed according to V / V=1:1, extracted for 30 minutes, and centrifuged at 2000rpm for 5 minutes to obtain the reverse micelles phase; the reverse micelles solution was chloroform with a volume ratio of 1:1 After mixing with n-butanol, add TOMAC to make a mixed solution with a final concent...

Embodiment 3

[0069] Weigh 100g of ground fungus powder, add 25 times the amount of 0.7mol / L ionic liquid, and extract at room temperature for 50 minutes; at 200w ultrasonic power, 10min ultrasonic time, 200w microwave power, microwave heating adopts intermittent treatment, heating 1min, intermittent 1min, microwave Treat for 5 minutes, centrifuge at 5000 rpm for 20 minutes to obtain supernatant;

[0070] Add ammonium sulfate to the supernatant to make the saturation 70%, let stand at 8°C for 2 hours, centrifuge at 7000rpm for 15 minutes, and take the supernatant to obtain the crude extract of Auricularia polysaccharide;

[0071] The crude polysaccharide extract and the reverse micelles solution were fully mixed according to V / V=2:1, extracted for 60 minutes, and centrifuged at 3000rpm for 10 minutes to obtain the reverse micelles phase; wherein the reverse micelles solution was chloroform with a volume ratio of 4:1 After mixing with n-butanol, add TOMAC to make a mixed solution with a conc...

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Abstract

The invention belongs to the field of nostoc commune polysaccharide extraction, and concretely relates to an extraction method. The method comprises the following steps: preparing a nostoc commune powder suspending liquid, preparing a nostoc commune cell disruption liquid, preparing a nostoc commune polysaccharide crude extract, and pre-extracting the material to obtain a reversed micelle phase, taking the separated water phase as the ionic liquid, removing the ionic liquid and performing cycle utilization and back extraction to prepare the nostoc commune polysaccharide, taking the recovered reversed micelle phase as a reversed micelle solution for cycle utilization and pre-extraction again, taking a supernatant generated after alcohol precipitation and separation of the water phase for effecting the ionic liquid, and performing recovery and cycle utilization. The method has the advantages of simple operation and repeatable used raw materials, can reduce the production cost of the nostoc commune polysaccharide, keeps high extraction yield and recovery rate, and is in favor of an application of polysaccharide in food industry.

Description

technical field [0001] The invention belongs to the field of extracting auricularia polysaccharides, and in particular relates to a method for extracting auricularia polysaccharides. Background technique [0002] Ground vegetables, also known as ground ears, lichens, ground fungus, etc., are a combination of fungi and algae, generally growing in dark and humid places, dark black, a bit like soaked black fungus. Peeled vegetables are rich in protein, multivitamins, phosphorus, zinc, calcium and other minerals. Scientists from Weizmann, Israel, have found that a component contained in wild vegetables can inhibit the activity of acetylcholinesterase in the human brain, thereby Can produce curative effect on senile dementia. [0003] Polysaccharides refer to a class of natural macromolecular compounds composed of more than 10 monosaccharide molecules, and are one of the macromolecular substances that maintain normal life activities. [0004] Studies have shown that the fungus ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 罗光宏张喜峰杨生辉王丹霞陈天仁
Owner HEXI UNIV
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