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A kind of preparation method of Dictyophora mycotopolysaccharide with antioxidant activity

A technology of anti-oxidative activity and polysaccharides, applied in the direction of anti-toxic agents, drug combinations, etc., to achieve synergistic effects of anti-oxidative function, acceleration of polysaccharide extraction, and strong efficacy

Active Publication Date: 2022-06-17
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the technical research on the use of tea soup to extract the polysaccharides with antioxidant activity in the fungus receptacle of Dictyophora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] A kind of preparation method of the Pseudomonas spp. polysaccharide with antioxidant activity, comprises the steps:

[0029] (1) Preparation of tea soup: Select dry old selenium-enriched tea leaves, remove foreign matter such as weeds, add pure water according to the weight ratio of material to liquid 1:30, boil for 50 minutes, filter with 40 mesh screen to obtain tea soup, use 3% food Grade citric acid solution to adjust the pH of the tea soup to 4.5, for later use.

[0030] (2) Bamboo fungus tray pretreatment: soak the dried bamboo fungus tray with 75% food-grade alcohol for 48 hours, dry to constant weight, pulverize through a 40-mesh sieve, and set aside for subsequent use;

[0031] (3) Extraction of the polysaccharide from the bamboo fungus receptacle: the above dried bamboo fungus receptacle powder is added to the tea soup at a mass ratio of 1:30 to the liquid, and extracted at a constant temperature of 60 ° C for 3 hours;

[0032] (4) Separation of the polysacch...

Embodiment 2

[0036] A kind of preparation method of Pseudomonas spp. polysaccharide with antioxidant activity, comprising the steps:

[0037] (1) Preparation of tea soup: Select dry old leaves of selenium-enriched tea, remove foreign matter such as weeds, add pure water according to the weight ratio of material to liquid 1:40, boil for 40 minutes, filter with 40 mesh screen to obtain tea soup, use 2% food Grade citric acid solution to adjust the pH of the tea soup to 5.0 for use.

[0038] (2) Bamboo fungus tray pretreatment: soak the dried bamboo fungus tray with 75% food-grade alcohol for 48 hours, dry to constant weight at 45°C, pulverize through a 40-mesh sieve, and set aside for subsequent use;

[0039] (3) Extraction of the polysaccharide from the bamboo fungus receptacle: the above dried bamboo fungus receptacle powder was added to the tea soup at a mass ratio of 1:50 to the liquid, and was extracted at a constant temperature of 70 ° C for 2 hours;

[0040] (4) Separation of the pol...

Embodiment 3

[0044] A kind of preparation method and application of P. chinensis polysaccharide with antioxidant activity, comprising the steps:

[0045] (1) Preparation of tea soup: Select dry old selenium-enriched tea leaves, remove foreign matter such as weeds, add pure water according to the weight ratio of material to liquid 1:50, boil for 60 minutes, filter through a 40-mesh sieve to obtain tea soup, use 1% food Grade citric acid solution to adjust the pH of the tea soup to 5.5 and set aside.

[0046] (2) Bamboo fungus tray pretreatment: soak the dried bamboo fungus tray with 75% food-grade alcohol for 48 hours, dry to constant weight at 45°C, pulverize through a 40-mesh sieve, and set aside for subsequent use;

[0047] (3) Extraction of the polysaccharide from the bamboo fungus receptacle: the above dried bamboo fungus receptacle powder is added to the tea soup at a mass ratio of 1:40 to the liquid, and extracted at a constant temperature of 80°C for 4 hours;

[0048] (4) Separatio...

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Abstract

The present invention relates to the processing field of edible fungi, in particular to a method for preparing Dictyophora mycotrichum polysaccharides with antioxidant activity, comprising the steps of degreasing, pulverizing, extracting, separating, removing protein, and purifying, and is characterized in that the extracting Using the selenium-enriched tea soup with pH 4.5-5.5 as a solvent to extract the fungus receptacle of bamboo fungus at a constant temperature; the invention uses the old leaves of waste planted in tea and the fungus receptacle of waste cultivation of bamboo fungus to extract polysaccharides with antioxidant activity, which can Increasing the extraction rate of polysaccharides has a synergistic effect on the anti-oxidation function of polysaccharides, and the extracted polysaccharides have stronger anti-oxidation and free radical scavenging effects; the preparation process of the invention is simple, the product is safe, and it can be produced on a large scale, and can turn waste into treasure. Protecting the environment has good economic, social and ecological benefits.

Description

technical field [0001] The invention relates to the processing field of edible fungi, in particular to a method for preparing a polysaccharide with antioxidative activity. Background technique [0002] Bamboo fungus is a fungus belonging to the Phalloidinaceae family, with high edible and medicinal value. It is the main economic edible fungus widely grown in Yunnan, Sichuan, Guizhou, Fujian and other places. The whole plant of bamboo fungus is composed of susceptor, stipe, and fungus cap. Commercial bamboo fungus is the fungus stipe and fungus cap. The total amount is more than 14%, the protein content is more than 23%, and it also contains trace elements such as calcium, iron, and zinc, which have high economic value. Environmental pollution. [0003] Existing studies have shown that the main physiological functions of bamboo fungus polysaccharides are: (1) regulating immune function; (2) inhibiting tumor and cancer cells; (3) anti-oxidation, delaying aging; (4) lowering ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003A61P39/06
Inventor 杨玲罗丽平康超王万坤郑旋杨彝华
Owner GUIZHOU INST OF BIOLOGY
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