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Carrot seed antioxidant tripeptide as well as preparation method and application thereof

A carrot seed and oxidized tripeptide technology, applied in the field of health food, can solve the problems of carrot seed protein and active peptide exploration, and achieve the effects of high stability, strong acid resistance and strong alkali resistance

Inactive Publication Date: 2016-01-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on carrot seed mainly focuses on carrot seed oil, carotene and flavonoids, etc., and there is no related exploration on carrot seed protein and active peptides

Method used

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  • Carrot seed antioxidant tripeptide as well as preparation method and application thereof
  • Carrot seed antioxidant tripeptide as well as preparation method and application thereof
  • Carrot seed antioxidant tripeptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The separation and purification of the antioxidant tripeptide in the present invention includes two steps of SephadexG-25 gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).

[0021] Preparation of enzymatic hydrolyzate of carrot seed protein: Carrot seed protein was obtained by extraction and acid precipitation method, and acidic protease was used to hydrolyze it under the optimal enzymatic hydrolysis conditions (enzyme addition 419.36U / g, substrate concentration 5.28% (w / v)) The enzymatic hydrolysis time is 3.50 hours, inactivated in a boiling water bath for 10 minutes, centrifuged at 12000 rpm for 20 minutes, and the supernatant is freeze-dried, which is the enzymatic hydrolyzate of leek seed protein;

[0022] SephadexG-25 gel filtration chromatography: redissolve the lyophilized powder of carrot seed protein hydrolyzate in deionized water, centrifuge at 12000rpm for 15min, and remove the supernatant. The SephadexG-25 ge...

Embodiment 2

[0027] The antioxidant tripeptide obtained in Example 1 was subjected to solid-phase synthesis, and the activity of the synthesized polypeptide was studied:

[0028] ABTS free radical scavenging effect: prepare 7mmol / L ABTS with distilled water + solution and 2.45mmol / L potassium persulfate solution (must be placed at room temperature for 16h before use), ABTS + Mix with potassium persulfate solution at a volume ratio of 1:1, and dilute the mixture to the absorbance value A with pH 7.4, 5 mmol / L phosphate buffer solution before use 734 is 0.70±0.02. Take 0.5mL of samples with different concentrations and 0.5mL of ABTS free radical solution, mix and let stand for 10min, then measure the absorbance value at 734nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. ABTS free radical scavenging activity is calculated according to the following formula (1):

[0029]

[0030] In the formula, A 0 : Absorba...

Embodiment 3

[0033] Stability of the antioxidant tripeptide of the present invention:

[0034] Carrot seed antioxidant peptide YSL was heat-treated at 20°C-100°C for 1h-5h, and the ABTS free radical scavenging rate was measured every 1h. Such as Figure 5, it was determined that the free radical scavenging rate of YSL had no significant change after being treated at 20°C, 40°C and 60°C for 5 hours (p>0.05). The carrot seed antioxidant peptide YSL was treated with different pH deionized aqueous solutions (2.0, 4.0, 6.0, 8.0 and 10.0) for 1h~5h, and the ABTS free radical scavenging rate was measured every 1h. It has been determined that the tripeptide YSL has strong alkali resistance and strong acid resistance. Although the activity decreases under acidic conditions, its structure is not destroyed and can still maintain activity ( Image 6 ).

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Abstract

The invention discloses a carrot seed antioxidant tripeptide as well as a preparation method and an application thereof. The sequence of the carrot seed antioxidant tripeptide is Tyr-Ser-Leu (YSL). The in-vitro experiment shows that DPPH and ABTS free radicals can be effectively removed by the tripeptide, and meanwhile the tripeptide is relatively good in stability. The carrot seed antioxidant tripeptide disclosed by the invention has the characteristics of simple structure, high antioxidant activity, and the like, can be used as assistant and substitute of an artificially synthesized antioxidant, and has important values for development and application of novel antioxidant healthcare products.

Description

Technical field [0001] The invention involves a natural antioxidant trice peptide and its preparation methods and the application in the field of health products. It belongs to the field of health food. Background technique [0002] As a large agricultural country, there are many types of crops and rich output.Due to the limitation of the technical conditions of agricultural product processing, most of the processing of crops is in the initial stage.Some crops have large content of protein in the residual objects, and most of them are mainly used for the feed industry or natural emissions, which not only wastes resources, but also cause environmental pollution.The proposal of the concept of plant activated peptide and the improvement of separation detection technology has enabled people to gradually pay attention to the deep processing of agricultural and sideline products rich in protein and obtain a variety of active peptides from it The research and development of plant source...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/087C12P21/06C07K1/16C07K1/20A23L33/00
Inventor 洪晶户佩胡磊孟春杨娟娟叶南慧
Owner FUZHOU UNIV
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