Spectrum-effect relationship-based propolis quality control method

A quality control method and technology of propolis, applied in color/spectral property measurement, measurement device, material analysis by observing the influence on chemical indicators, etc., can solve the problems of quality and efficacy decline, uneven quality of propolis and other problems

Active Publication Date: 2013-03-20
GUANGZHOU CITY TANSHAN APICULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality levels of propolis currently on the market are uneven, resulting in a significant decline in the quality and effica

Method used

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  • Spectrum-effect relationship-based propolis quality control method
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  • Spectrum-effect relationship-based propolis quality control method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] (1) Instruments and reagents: Waters ALLIANCE high performance liquid chromatography (E2695 quaternary pump, autosampler, column thermostat, 2998 diode array detector, vacuum degasser, Empower3 Chromatography Workstation); BP211D electronic balance from Sedlis, Germany; Luna C from Phiromens, USA 18(2） Chromatography column (4.6mm×250mm, 5μm). Methanol is chromatographically pure, and other reagents are analytically pure.

[0071] (2) Test solution: Take propolis medicinal material powder, accurately weigh about 1.0000g, put it in a Soxhlet extractor, extract it with 100mL acetone Soxhlet until it is colorless, evaporate the extract to dryness in a water bath, dissolve the residue in methanol, and place in a Soxhlet extractor. In a 100mL measuring bottle, dilute to the 100mL mark with methanol.

[0072] (3) The mobile phase is: A: methanol, B: phosphoric acid solution with a concentration of 0.4% by volume; gradient elution method: 0min→10min→20min→30min→38min→56min→6...

Embodiment 2

[0093] (1) Instruments and reagents: Waters ALLIANCE high performance liquid chromatography (E2695 quaternary pump, autosampler, column thermostat, 2998 diode array detector, vacuum degasser, Empower3 Chromatography Workstation); BP211D electronic balance from Sedlis, Germany; Luna C from Phiromens, USA 18(2） Chromatography column (4.6mm×250mm, 5μm). Methanol is chromatographically pure, and other reagents are analytically pure.

[0094] (2) Test solution: Take propolis medicinal material powder, accurately weigh about 1.0000g, put it in a Soxhlet extractor, extract it with 100mL acetone Soxhlet until it is colorless, evaporate the extract to dryness in a water bath, dissolve the residue in methanol, and place in a Soxhlet extractor. In a 100mL volumetric flask, dilute methanol to the mark.

[0095] (3) The mobile phase is: A: methanol, B: phosphoric acid solution with a concentration of 0.4% by volume; gradient elution method: 0min→10min→20min→30min→38min→56min→67min→72min→...

Embodiment 3

[0117] (1) Instruments and reagents: Waters ALLIANCE high performance liquid chromatography (E2695 quaternary pump, autosampler, column thermostat, 2998 diode array detector, vacuum degasser, Empower3 Chromatography Workstation); BP211D electronic balance from Sedlis, Germany; Luna C from Phiromens, USA 18(2） Chromatography column (4.6mm×250mm, 5μm). Methanol is chromatographically pure, and other reagents are analytically pure.

[0118] (2) Test solution: Take propolis medicinal material powder, accurately weigh about 1.0000g, put it in a Soxhlet extractor, extract it with 100mL acetone Soxhlet until it is colorless, evaporate the extract to dryness in a water bath, dissolve the residue in methanol, and place in a Soxhlet extractor. In a 100mL volumetric flask, dilute methanol to the mark.

[0119] (3) The mobile phase is: A: methanol, B: phosphoric acid solution with a concentration of 0.4% by volume; gradient elution method: 0min→10min→20min→30min→38min→56min→67min→72min→...

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Abstract

The invention provides a spectrum-effect relationship-based propolis quality control method. The spectrum-effect relationship-based propolis quality control method can forecast the antioxidant activity of propolis according to a propolis fingerprint. The spectrum-effect relationship-based propolis quality control method utilizes high performance liquid chromatography (HPLC) to build a propolis fingerprint and realizes evaluation of antioxidant activity according to potassium permanganate fading time, a DPPH free radical clearance rate, a phosphomolybdic acid binding rate and a hydroxyl radical clearance rate. Through analysis of a propolis fingerprint chromatograph and spectral characteristics, the spectrum-effect relationship-based propolis quality control method determines 25 characteristic peaks and 5 characteristic peak groups. Through fitting of a polynomial, the spectrum-effect relationship-based propolis quality control method builds a mathematical model for forecasting the antioxidant activity of propolis according to a propolis fingerprint. According to the characteristic peaks and the characteristic peak groups, only through the mathematical model, the spectrum-effect relationship-based propolis quality control method can directly determine strength of the antioxidant activity of a propolis drug without an anti-oxidation test thereby determining quality. The spectrum-effect relationship-based propolis quality control method has the advantages of simple processes, strong objectivity and more characteristic peaks and realizes perfect and scientific propolis quality control.

Description

【Technical field】 [0001] The invention belongs to the technical field of quality standards of traditional Chinese medicines, in particular to a propolis quality control method based on spectrum-effect relationship for predicting the antioxidant activity of propolis samples to evaluate their quality. 【Background technique】 [0002] Propolis is a resin collected by worker bees from new shoots or branches of plants, mixed with bee mandibular gland secretions and beeswax, and processed into a sticky, aromatic solid jelly. Propolis contains bioactive substances such as flavonoids, terpenes, phenols and esters, among which flavonoids have significant antioxidant activity. Among the 62 health foods newly approved by the State Food and Drug Administration in 2011 with bee products as raw materials, there are 57 health products with propolis as raw materials, accounting for 91.94% of all bee product health foods. The status of propolis health food in my country’s bee product health ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/78G01N21/31
Inventor 林励陈南迪谭东山
Owner GUANGZHOU CITY TANSHAN APICULTURE
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