Indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus and application thereof

A detection kit, Bacillus technology, applied in measuring devices, antibacterial immunoglobulins, instruments, etc., can solve the problem of short detection time, and achieve the effects of short detection time, high specificity, and simple operation.

Inactive Publication Date: 2012-11-07
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no indirect ELISA detection method for Alicyclobacillus at home and abroad. Compared with

Method used

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  • Indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus and application thereof
  • Indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus and application thereof
  • Indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus and application thereof

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Embodiment Construction

[0045] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0046] 1 Materials and methods

[0047] 1.1 Strains and serum

[0048] Alicyclobacillus was purchased from the German Culture Collection (No. DSM3299T ); the positive serum and negative serum of Alicyclobacillus were self-made serums.

[0049] 1.2 Main reagents

[0050] Horseradish peroxidase-labeled goat anti-rabbit IgG was purchased from Shanghai Sangon Bioengineering Co., Ltd., 96-well detachable ELISA plate was purchased from Nunc Company; BSA was purchased from Sigama Company; other reagents were imported or domestically analyzed pure.

[0051] 1.3 Experimental animals

[0052]Two healthy adult white rabbits, male, were purchased from the Experimental Ani...

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Abstract

The invention discloses an indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus, which comprises components (1)-(10). Simultaneously, the invention further discloses a using method of the indirect ELISA kit for alicyclobacillus. The indirect ELISA kit for alicyclobacillus provided by the invention fills in the blank that an ELISA method against alicyclobacillus is not established in China, and further has higher sensitivity and specificity.

Description

technical field [0001] The invention relates to an ELISA kit, in particular to an indirect ELISA detection kit for Alicyclobacillus. Background technique [0002] For the detection of Alicyclobacillus, the methods of bacterial isolation and biochemical identification are usually used. This method takes a long time to detect, is costly, and has high requirements for the operator's experimental level, which cannot meet the actual requirements of daily production monitoring. The indirect ELISA detection method adopted in the present invention is an improvement of the immunofluorescence method, and has the advantages of fast qualitative and quantitative, high sensitivity, wide application range, objective result judgment, low cost and the detection of thousands of samples at the same time, so the method It has been released, and it has been rapidly promoted. Therefore, ELISA detection methods for foodborne microorganisms such as Salmonella, E. coli 0157:H7, Shigella, and Staph...

Claims

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Application Information

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IPC IPC(8): G01N33/569C07K16/12C07K16/06
Inventor 刘磊张亮郭玉蓉
Owner GANSU AGRI UNIV
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