Microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore
A technology for manganese oxide ore and valuable metals, applied in the field of microbial metallurgical beneficiation, can solve the problems of slow growth and low quantity, and achieve the effects of high total biomass, improved efficiency and fast growth
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[0021] Example 1: Using pyrite as a donation of ferrous sulfate
[0022] 1. The domestication of leaching bacteria
[0023] (1) Expanded culture of leaching bacteria
[0024] Inoculate the Alicyclobacillus BM strain into the culture medium and cultivate it at 25~35℃ to OD 600 Stop the culture when it reaches 0.8-1.0, and get the expanded culture solution of BM strain;
[0025] The medium is BAM medium. In order to increase the carbon source of the culture medium and adapt the BM strain to a higher concentration of ferrous ion solution, glucose with a mass percentage of 0.5% to 1% and a mass of 4-20% ferrous sulfate were added to the BAM medium. Iron; adjust the pH of the medium to 1.5 to 5. The culture is cultured in a shaking flask with a rotation speed of 100 rpm to 180 rpm and a culture temperature of 25 to 35°C.
[0026] (2) Domestication of leaching bacteria:
[0027] a. Domesticating bacteria in seed solution to adapt to pyrite
[0028] Based on the BAM culture solution, add 0.5%...
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