The invention belongs to the technical field of
cell immobilization, and particularly relates to an acidophilic
thiobacillus ferrooxidans immobilization material and application thereof in
cell immobilization and desulfurization. According to the
acidithiobacillus ferrooxidans immobilized material, Fe3O4 nano-clusters are used as raw materials, a PDA shell layer with a mesoporous structure is formed on the surfaces of the Fe3O4 nano-clusters by means of self-
polymerization reaction of
dopamine, a nano
composite material Fe3O4 (at) mPDA is constructed, meanwhile, biotinylated
chitosan derivatives Bio-CS are synthesized by using
biotin as raw materials, and the
acidithiobacillus ferrooxidans immobilized material is prepared. And combining the Fe3O4 (at) mPDA with a biotinylated
chitosan derivative Bio-CS by virtue of an emulsification method, thereby obtaining the Fe3O4 (at) mPDA. The
acidithiobacillus ferrooxidans immobilized material provided by the invention can be used for immobilizing
acidithiobacillus ferrooxidans. The material provided by the invention not only can effectively maintain the
biological activity of the
acidithiobacillus ferrooxidans BY3, but also has the advantages of mild
reaction conditions, no secondary
pollution and capability of maintaining the cyclic utilization of Fe < 3 + >, so that the method meets the manufacturing requirements of a green
biotechnology.