15 results about "Acidithiobacillus ferrooxidans" patented technology

The invention relates to the technical field of metallurgy, in particular to an efficient-utilization copper-cobalt ore dump leaching treatment method which comprises the steps that after copper-cobalt sulfide ore and copper-cobalt oxide ore are subjected to crushing treatment respectively, heap building is conducted; a solution of acidithiobacillus ferrooxidans is added into a sulfuric acid solution to serve as spraying liquid, heap leaching treatment is conducted on the heap-built copper-cobalt ore through a spraying device, and the spraying flow of the spraying device is controlled in the heap leaching treatment process according to changes of the environment temperature and humidity and changes of the pH value of heap leaching liquid; the obtained dump leaching liquid is subjected to extraction-electrodeposition treatment, and cathode metal copper, sediment and raffinate are obtained; and the raffinate serves as spraying liquid to be subjected to spraying circulation, precipitation is neutralized, and metal cobalt is recycled. The spraying flow of the spraying device is accurately controlled and adjusted, and the leaching effect of copper and cobalt metal in the copper-cobalt ore is improved.

The invention discloses a method for biologically leaching a retired ternary lithium battery positive electrode material under the catalysis of silver ions, which comprises the following steps of: (1) discharging, roasting, disassembling and grinding a retired ternary lithium battery to obtain ternary lithium battery positive electrode material powder; (2), Acidithiobacillus ferrooxidans is inoculated into the culture medium, and when the redox potential in a culture system is larger than or equal to 600 mV, a bacterial culture solution is obtained; and (3) adding the waste ternary lithium battery positive electrode powder into a bacterial culture solution, then adding a silver nitrate solution for leaching, and culturing for 3-7 days to obtain a biological leaching solution. According to the method, the biological leaching rate of metal is increased through silver ions, operation is easy and convenient to implement, the catalyst is simple and easy to obtain, the production cost is low, and environmental pollution is small, and the method has good application prospects.

The invention discloses a fluoride-free flotation-microbiological leaching-multistage magnetic separation synergistic tailing purification process, which takes mine tailings as raw materials and is realized by the following steps: 1, fluoride-free flotation desliming and impurity removal: replacing hydrofluoric acid with a novel anion collector (fatty acid derivative), and preferentially removing mica and feldspar under the condition that the pH is 2.5-3.5; 2, removing iron and aluminum by microbiological leaching: leaching for 48 hours at the temperature of 35 DEG C by utilizing an acidophilus bacillus ferroxidans bacterial solution, and dissolving Fe2O3 and Al2O3 in a wrapped state; 3, high-gradient magnetic separation refining: removing magnetic minerals through 1.2 T strong magnetic separation, and then separating weak magnetic impurities through 0.8 T weak magnetic separation; the indexes of the final product are as follows: the purity of SiO2 is greater than or equal to 99.9%, the whiteness is greater than or equal to 94% (ISO standard), the content of Fe2O3 is less than or equal to 80ppm, Al2O3 is less than or equal to 1200ppm, and the requirements of electronic grade (IOTA-4 standard) quartz powder are met.

The invention discloses a method for enhancing biological leaching of copper in a waste circuit board by utilizing magnetite. The method comprises the following steps: adding the pre-crushed waste circuit board and magnetite into a 9K culture medium with the pH value of 1.8-2.0 according to a certain mass ratio, inoculating Acidithiobacillus ferrooxidans as a leaching strain, and carrying out a leaching reaction under a constant-temperature oscillation condition. The magnetite is added, so that the leaching of copper in the circuit board is remarkably enhanced, the leaching rate is remarkably improved, and the leaching period is shortened. And compared with the microbiological leaching result without magnetite, the copper leaching rate is improved by 12-32%. By utilizing the difference between the circuit board and the magnetite in particle size, the magnetite can be recycled from the leaching residues through a physical separation means for a new round of leaching. The method is simple in process, the strain is easy to culture, and the method has good environmental friendliness and is suitable for green and efficient recovery of copper in the waste circuit board.

The invention discloses a method for recycling vanadium through biological dump leaching by using fluorine-resistant acidophilic ore leaching bacteria. According to the technical scheme, an acidophilic ore leaching bacterium strain named as Acidithiobacillus ferrooxidans is adopted, a fluorine-resistant acidophilic ore leaching bacterium solution is obtained through domestication, and the F <->-resistant content of the fluorine-resistant acidophilic ore leaching bacterium solution reaches 0.36-2.7 g / L. The fluorine-resistant acidophilic mineral leaching bacteria liquid preserved at the ultralow temperature is subjected to expanding culture, and the fluorine-resistant acidophilic mineral leaching bacteria liquid subjected to expanding culture is obtained. And roasting the vanadium shale, building an ore heap, and intermittently spraying sulfuric acid to the ore heap. And then a fluorine-containing leaching aid is added into the fluorine-resistant acidophilic ore leaching bacterium liquid subjected to expanding culture to obtain a fluorine-containing acidophilic ore leaching bacterium liquid, the fluorine-containing acidophilic ore leaching bacterium liquid is intermittently dripped and sprayed to the ore heap subjected to sulfuric acid spraying for heap leaching, a leaching solution is obtained, and vanadium recovery is further achieved. The acidophilic ore leaching bacteria are subjected to fluorine-resistant domestication and enlarged culture, and are used for heap biological leaching of the low-grade vanadium shale, so that the leaching rate is high, and the treatment capacity is large.

The invention belongs to the technical field of cell immobilization, and particularly relates to an acidophilic thiobacillus ferrooxidans immobilization material and application thereof in cell immobilization and desulfurization. According to the acidithiobacillus ferrooxidans immobilized material, Fe3O4 nano-clusters are used as raw materials, a PDA shell layer with a mesoporous structure is formed on the surfaces of the Fe3O4 nano-clusters by means of self-polymerization reaction of dopamine, a nano composite material Fe3O4 (at) mPDA is constructed, meanwhile, biotinylated chitosan derivatives Bio-CS are synthesized by using biotin as raw materials, and the acidithiobacillus ferrooxidans immobilized material is prepared. And combining the Fe3O4 (at) mPDA with a biotinylated chitosan derivative Bio-CS by virtue of an emulsification method, thereby obtaining the Fe3O4 (at) mPDA. The acidithiobacillus ferrooxidans immobilized material provided by the invention can be used for immobilizing acidithiobacillus ferrooxidans. The material provided by the invention not only can effectively maintain the biological activity of the acidithiobacillus ferrooxidans BY3, but also has the advantages of mild reaction conditions, no secondary pollution and capability of maintaining the cyclic utilization of Fe < 3 + >, so that the method meets the manufacturing requirements of a green biotechnology.

The application discloses a composite bactericide and application, which comprises cetyltrimethylammonium bromide, 5-chloro-2-methyl-4-isothiazolin-3-one, tetramethylammonium phosphonium sulfate, benzalkonium chloride, wherein the mass concentration of the cetyltrimethylammonium bromide is 0.2-0.5 mg / L, the mass concentration of the tetramethylammonium phosphonium sulfate is 0.4-1 mg / L, the mass concentration of the 5-chloro-2-methyl-4-isothiazolin-3-one is 0.2-0.5 mg / L, and the mass concentration of the benzalkonium chloride is 0.2-0.5 mg / L. The composite bactericide can simultaneously effectively kill Acidithiobacillus ferrooxidans A. ferrooxidans and common desulfurization vibrio D. vulgaris with a sterilization rate of 98%, and has a synergistic sterilization effect.

The present application relates to a kind of composite strains and its application in coal gangue and acid mine drainage treatment and rare earth leaching, belong to environmental microbial repair, mine solid waste resource and rare earth biological leaching technical field.The composite strain of the present application includes Acidithiobacillus ferrooxidans WZ33 and acid deposit acidophilic thiobacillus WY33;The preservation number of the Acidithiobacillus ferrooxidans is: CGMCC No.37726;The preservation number of the acid deposit acidophilic thiobacillus is CGMCC No.38196.Acidithiobacillus ferrooxidans (A. ferrooxidans WZ33) in the present application is mainly responsible for ferrous oxidation, rapid acid production and Fe 3+ Oxidizing agent is generated;Acid deposit acidophilic thiobacillus (A. acidisediminis WY33) is mainly responsible for sulfur material oxidation, eliminate sulfur passivation film, continuously maintain low pH environment.The two form complementary synergic system, significantly improve leaching and processing efficiency, better than single strain.

Methods and genomic editing systems for Acidithiobacillus ferrooxidans are described herein involving use of Cpf1 (Cas12a).

This application relates to the field of metallurgical technology, and specifically relates to a method for heap leaching treatment of copper-cobalt ore with high efficiency utilization. The method includes: after respectively crushing sulfide copper-cobalt ore and oxide copper-cobalt ore, building a heap; adding a solution of Acidithiobacillus ferrooxidans to sulfuric acid solution as a spraying liquid, and performing heap leaching treatment on the copper-cobalt ore after building the heap through a spraying device. During the heap leaching treatment process, according to the changes in ambient temperature and humidity and the change in the pH value of the heap leaching solution, controlling the spraying flow rate of the spraying device; performing extraction-electrowinning treatment on the obtained heap leaching solution to obtain cathode metallic copper, precipitate and raffinate; using the raffinate as a spraying liquid for spraying circulation, performing neutralization treatment on the precipitate, and recovering metallic cobalt. This application aims to accurately control and adjust the spraying flow rate of the spraying device to improve the leaching effect of copper and cobalt metals in copper-cobalt ore.

This application discloses an algae-bacterial symbiotic system and its application in accelerating iron biomineralization in acidic mine wastewater, belonging to the field of acidic mine wastewater treatment technology. By simultaneously inoculating *Parachlorella sp. AH02* and *Acidithiobacillus ferrooxidans* LX5, supplemented with a small amount of nutrients, light, and oxygenation, an algae-bacterial symbiotic reaction system is formed. Based on the dual effects of electron shuttle in algal-derived organic matter and seed crystal template regulation by algal cells, high-concentration dissolved Fe(II) in acidic mine water is rapidly oxidized to Fe(III), and Fe(III) is rapidly hydrolyzed to ferric hydroxide minerals, thereby significantly improving the biomineralization efficiency of Fe and contributing to the green and efficient treatment of acidic mine wastewater.

This application provides a method for enhanced bioleaching of uranium ore based on sulfur autotrophic denitrification pre-desulfurization, comprising: placing uranium ore in a reactor, inoculating with sulfur autotrophic denitrifying bacteria, and introducing nitrate-containing feed water under anoxic conditions; utilizing the denitrifying bacteria to selectively oxidize and remove the sulfide coating layer on the ore surface by using sulfide-iron compounds in the uranium ore as electron donors and nitrates as electron acceptors, to obtain desulfurized uranium ore; then placing the desulfurized uranium ore in a leaching system and inoculating with *Acidithiobacillus ferrooxidans* for bioleaching to achieve uranium extraction. This invention selectively removes the sulfide coating layer on the uranium ore surface through sulfur autotrophic denitrification pre-desulfurization treatment, fully exposing the uranium minerals and significantly improving bioleaching efficiency. After optimized process treatment, the uranium leaching rate increased from 41.2% in the untreated ore to 66.5%. This method has high selectivity and low operating costs, providing a new technical path for the efficient extraction of low-grade, complex, associated uranium ores.

A method to increase bioleaching efficiency comprising contacting a modified Acidithiobacillus ferrooxidans with pyrite, chalcopyrite or combination thereof, wherein the bioleaching efficiency is increased as compared to a wild type Acidithiobacillus ferrooxidans. The modified Acidithiobacillus ferrooxidans can comprises one or more mutations in the petB2 gene (in petll operon) and / or the petBl gene (in petl operon) of A. ferrooxidans so as to knock down or knock out expression of petB2 or petB 1. Considering that the dPetB2 cells can still oxidize sulfur, these cells can overcome restricted chalcopyrite bioleaching through accelerated iron oxidation, while maintaining levels of sulfur oxidation. This advantage can be reinforced by the reduced formation ofjarosite. An increase in the contribution of the non-contact iron oxidation over the direct contact oxidation mechanism can enhance the bioleaching of iron sulfide ores.

The invention provides a rapid analysis method of metal corrosive microorganism acidithiobacillus ferrooxidans, and belongs to the technical field of biology.The rapid analysis method comprises the steps that a microorganism genome DNA sample to be detected and a photosensitizer As2MoO6 at-NiS are taken to jointly construct a triple asymmetric photon PCR system, asymmetric photon PCR amplification is carried out under the condition of LED light source excitation, and the amplification result is obtained; after amplification is completed, the obtained amplification product is mixed with a photo-thermal signal probe prepared from a photosensitizer As2MoO6 at NiS, and photo-thermal temperature change detection is carried out. The rapid analysis method has the characteristics of high efficiency, convenience and accuracy, and is suitable for rapid detection of acidithiobacillus ferrooxidans.

The application relates to the technical field of electronic material preparation, and particularly discloses a lead-ruthenate preparation method based on acidithiobacillus ferrooxidans mediation. The application takes soluble lead salt and soluble ruthenium salt as raw materials, utilizes the biological metabolic characteristics of acidithiobacillus ferrooxidans, regulates the oxidation-reduction environment and ion adsorption-deposition behavior of the system, realizes the directional synthesis of a lead-ruthenate precursor under mild conditions, and obtains high-purity and high-crystallinity lead-ruthenate powder through subsequent calcination treatment. The method has the advantages of mild reaction conditions, high raw material utilization rate, controllable product purity, green environmental protection and the like, solves the technical problems of high energy consumption of a traditional high-temperature solid-phase method, easy product agglomeration, complex process of a sol-gel method and high cost, and the prepared lead-ruthenate powder has good application prospects in the fields of thick-film resistors, high-temperature electrode materials and the like.