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398results about How to "Avoid recontamination" patented technology

Primer middle sequence interference PCR (Polymerase Chain Reaction) technology

The invention relates to a primer middle sequence interference PCR (Polymerase Chain Reaction) technology. The improved PCR technology is characterized in that one segment of relatively non-complemented or same-sequence basic group primer molecules in the intermediate domain of primers perform the antisense interference inside and outside so as to competitively destroy the polymerization among the primers to selectively inhibit the primer dimer (PD) from being amplified. For the interference of the intermediate domain of the primers, based on the primers optimally selected by the conventional design principle, the technology that the intermediate domain (ID) of a pair of the primers are in parallel but are not complemented with each other or are in the same sequence or/and the technology that ID antisense oligonucleotides (Oligo) are added into the primers to perform the interference action or/and the Oligo antonymy is carried out in the primer molecules via the ID so as to perform the interference action are adopted, or the combined technology of the three types of the technologies is adopted. As a result, only the ID of the primers is interfered while the target specific amplification is not influenced; the combining force acting on the minority of base-group pairing hydrogen bonds at the tail end and the base-group hydrogen bonds outside the tail end due to the action of the primers is dispersed to a maximum extent, so that the PD is selectively inhibited. Therefore, the PD accumulation in the PCR system is avoided. If the mineral oil is additionally used, the sealed primers can slowly release the hot starting and the UDG pretreatment so as to prevent aerosol glue as a byproduct of the PCR system from causing the pollution. Consequently, the nucleic acid is amplified reliably and the real-time fluorescence PCR is quantified accurately.
Owner:珠海市坤元科技有限公司

Intelligent fresh air and indoor air synchronous purification equipment and intelligent fresh air and indoor air synchronous purification method

The invention discloses a piece of intelligent fresh air and indoor air synchronous purification equipment and an intelligent fresh air and indoor air synchronous purification method. The equipment comprises a main case 13. The main case 13 is equipped with a fresh air inlet pipe 2, and the fresh inlet air pipe 2 is equipped with a valve device 1 used for opening and closing the air inlet pipe. The main case 13 is further equipped with an exhaust pipe 6, and the exhaust pipe 6 is equipped with a valve device 5 used for opening and closing the exhaust pipe. The main case is internally provided with an intelligent detection component 8 for air detection, a control component 9, an intake fan 17, an exhaust fan 7, an indoor circulation purification fan 14, an indoor air filtration and purification component 15, a fresh air filtration and purification component 3, a heat exchange component 4, an operation display component 12, and an indoor air quality display component 10. The equipment and the method of the invention can achieve a better purification effect, and are suitable for air purification under the condition of serious outdoor air pollution in our country at present stage.
Owner:BEIJING SYNTEC SCI & TECH DEV CO LTD

Cleaning machine with supporting and thrusting mechanism

The invention discloses a cleaning machine with a supporting and thrusting mechanism, which comprises a rack and a plurality of cleaning tanks, wherein a lifting bracket used for supporting material baskets is arranged in each cleaning tank; at least one side edge of each lifting bracket is connected with a lifting cross beam through a bracket connecting rod; a lifting driving mechanism mounted on the rack can drive the lifting brackets to be lifted to and fro in the vertical direction through the lifting cross beam; horizontal material basket push rods used for horizontally pushing the material baskets to move are further arranged above the cleaning tanks and are driven by a horizontal driving mechanism; fixed brackets are fixedly arranged between adjacent cleaning tanks; upper bearing surfaces of the fixed brackets are positioned in the same bearing plane as bearing surfaces of the lifting brackets lifted to high positions; and the bearing plane is higher than tank openings of the cleaning tanks. The cleaning machine has the advantages of stability and reasonableness in structure and great carrying capacity; and particularly, a cleaning and conveying path of the cleaning machine is reasonable, the effective cleaning time is long and the work efficiency is high.
Owner:SHANGHAI SNA ELECTRONICS TECH DONGTAI

Production method for aramid fiber 1414 paper substrate material

The invention relates to a production method for aramid fiber 1414 paper substrate material, which comprises the following steps of uniformly mixing aramid fiber 1414 chopped fiber size, modified thermoplasticity fiber size and aramid fiber 1414 pulp fiber size and then defibering; adding maleic anhydride-acrylic copolymer for mixed pulp dispersing; then regulating the concentration of the pulp and forming and hot pressing the obtained pulp suspension through manufacture paper with pulp so as to obtain aramid fiber 1414 paper substrate body paper; dipping the aramid fiber 1414 paper substrate body paper in polyimide resin glue solution, and removing volatile solvent after drying so as to obtain the aramid fiber 1414 paper substrate material. The preparation method is environment-friendly, metal ion can be prevented from contaminating the fiber again, the problem of a conventional dispersing agent generating bubbles in manufacture paper with pulp process is solved, the defect of adding a third fiber and organic resin alone is overcome, the physical property, resistance to high temperature and insulating property of the produced end product paper are greatly improved, and the functional index of the high performance aramid fiber 1414 paper substrate material can be fully achieved.
Owner:SHAANXI UNIV OF SCI & TECH

Cold-hot type rinsing air purification device using multilayer staggered spray method

The invention relates to a rinsing air purification technology and in particular relates to a cold-hot type rinsing air purification device using a multilayer staggered spray mode. The cold-hot type rinsing air purification device using the multilayer staggered spray method comprises a water storage tank, a spray tank, a water supply pipe, a water pump and an exhaust fan, wherein the water storage tank comprises a wastewater storage tank, a clear water storage tank, an electronic refrigerator, an electronic heater, an isolation plate and a wastewater filtration screen; the spray tank comprises a spray tank shell, a spray plate, a spray pipe, an air inlet, an air outlet and an air filtration screen; the spray plate, the spray pipe, the air inlet, the air outlet and the air filtration screen are arranged in a tank body; due to the adoption of the multilayer staggered spray method and a curve air flowing path, an equipment resource can be used to the greatest extent; air is fully sprayed and washed, so that the best purification effect can be achieved; the temperature of the air in a room can be adjusted according to the requirement during purifying of the air; the device can be used as an indoor moving air purifier, can be mounted on the wall to be used as an air purification window, and can be applied to spray purification towers for purifying industrial waste gas generated during waste incineration; the defects that a filter element is blocked and parts are frequently replaced can be avoided.
Owner:霍橡楠

Glass cleaning device

The invention discloses a glass cleaning device, and particularly relates to the technical field of cleaning. The glass cleaning device comprises a first support and a second support. The first support and the second support are arranged parallelly in the up-down direction, a plurality of upper rotating shafts and lower rotating shafts are arranged in the vertical directions of the first support and the second support, and the upper rotating shafts are opposite to the lower rotating shafts in an up-down mode; one ends of the upper rotating shafts are rotatably connected with the first support, one ends of the lower rotating shafts are rotatably connected with the second support, and disc brushes are arranged at the other ends of the upper rotating shafts and the lower rotating shafts correspondingly; and a lifting unit capable of enabling the first support to extend and retract in the vertical direction is arranged at one end of the second support, and sliding units which are matched with each other are arranged at the other end of the second support, wherein the other end of the second support is opposite to the lifting unit. According to the glass cleaning device, the two faces of glass can be cleaned simultaneously, the cleaning time is shortened, and glass cleaning is more thoroughly; and the situation that when the glass is turned over manually, the glass is polluted again is avoided, the labor cost is saved, and the efficiency is improved.
Owner:CHONGQING HUARUI GLASS CO LTD

Compound eluting agent for repairing organic matter and heavy metal compound contaminated soil, leaching method of compound eluting agent and eluting agent recycling process

The invention discloses an eluting agent for repairing organic matter and heavy metal compound contaminated soil, an eluting method of the compound eluting agent and an eluting agent recycling method,wherein the eluting agent is prepared by compounding Tween 80 and EDTA. The eluting method comprises the following steps: adding the eluting agent into the contaminated soil, oscillating, mixing, centrifugally separating an eluting solution, adding the compound eluting agent solution again into the soil subjected to first-time eluting, performing secondary elution, and repeating for four times. The eluting agent recycling method comprises the following steps: transferring the separated eluting solution into a photoelectric recovery device, adding sodium persulfate and sodium sulfate electrolytes, turning an ultraviolet lamp and a power supply for photoelectric treatment for 2.5 hours to selectively degrade organic matters in the eluting solution and recover heavy metals. The compound solution of the Tween 80 and EDTA-2 Na is used, a plurality of times of continuous eluting are carried out, a photoelectric synergistic persulfate method is adopted to regenerate the eluting agent, and the organic matter and heavy metal compound contaminated soil can be effectively repaired synchronously. The method has the characteristics of simple process, convenience operation, short time consumption, high efficiency and the like.
Owner:WUHAN UNIV
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