Microorganism detection device and detection method

Abstract

The invention discloses a microbiological detection device and method, and belongs to the technical field of microbiological detection.The microbiological detection device comprises a detector body and a shell half-wrapping the outer side of the detector body, a suction filtration component is arranged on the upper side of a movable base, and the suction filtration component comprises two suction filtration cylinders fixedly communicated with the upper side of the movable base; air suction pipes are inserted into the outer sides of the two suction filtration cylinders, the other ends of the two air suction pipes communicate with an external air suction pump, and the upper sides of the two suction filtration cylinders are sleeved with conveying cylinders. Samples retained in the filter membranes are extracted through the arranged suction filtration cylinder, layered filtration is performed by means of the different filter membranes, the samples are accelerated to penetrate through the filter membranes through suction force generated by the suction pipe in the suction filtration cylinder, and the diversity of impurities in the samples is effectively reduced through multi-layer filtration; therefore, the accuracy of a sample when the sample passes through the detector body is guaranteed, and meanwhile, the influence of an undissolved antibiotic solution and drugs on sample detection data is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism detection, and more specifically, to a microorganism detection device and a detection method. Background Art

[0002] Microbial detection instruments are important tools for identifying, counting, classifying and identifying microorganisms. They are widely used in many fields such as food, medicine, cosmetics, water quality testing, environmental monitoring and clinical medicine. The principles of microbial detection instruments are mainly based on a variety of scientific technologies, including but not limited to optics, biochemistry, immunology, electrochemistry and molecular biology. Common microbial detection instruments include ATP fluorescence detectors, microscopes, incubators, colony counters and liquid chromatographs.

[0003] Among them, optical principle detection uses a spectrum of a specific wavelength range to irradiate the sample, and infers the number of microorganisms by measuring parameters such as the sample's absorption, transmission or reflection of light; biochemical principle detection is based on the characteristics of microbial metabolic processes, such as enzyme reactions, affinity, and the binding of fluorescent molecules, so as to calculate the number and type of microorganisms; immunological principle detection uses the interaction characteristics of antibodies and antigens to detect microorganisms by generating detectable signals through the binding of specific antibodies to microbial antigens; and molecular biology principle detection uses PCR amplification technology combined with fluorescent dyes or fluorescent probes to achieve quantitative detection of microorganisms by detecting fluorescent signals;

[0004] In the prior art, when a microbial sample contains a large amount of non-target bacteria or contaminants, in order to effectively inhibit the growth of these bacteria, the sample may be pretreated with a highly antibacterial drug or antibiotic solution to reduce the interference of the bacteria on the test results and improve the detection rate of the target microorganisms. However, highly antibacterial drugs or solutions containing poorly soluble antibiotics will also directly affect the sample being tested, making it difficult to obtain accurate results. Summary of the invention

[0005] In view of the problems existing in the prior art, the purpose of the present invention is to provide a microbial detection device and a detection method, which can accelerate the sample to pass through the filter membrane, and the multi-layer filtration will effectively reduce the diversity of impurities inside the sample, while avoiding the influence of undissolved antibiotic solutions and drugs on the sample detection data.

[0006] To solve the above problems, the present invention adopts the following technical solutions.

[0007] A microorganism detection device, comprising a detector body and a shell half-wrapped outside the detector body, a slide groove is provided in the middle of the front end of the shell, the number of the slide grooves is multiple, the interiors of the multiple slide grooves are all slidably connected with sliders, the front faces of the multiple sliders are commonly fixedly connected with a moving seat, a suction filter component is provided on the upper side of the moving seat, and the suction filter component includes two suction filter cartridges fixedly connected to the upper side of the moving seat;

[0008] The outer sides of the two suction filter cylinders are connected with suction pipes, the other ends of the two suction pipes are connected with an external suction pump, the upper outer sides of the two suction filter cylinders are sleeved with conveying cylinders, the interior of the conveying cylinders is connected with a bracket, the interior of the brackets is overlapped with two filter membranes, the suction filter cylinders are connected with the suction pipes, the suction filter cylinders are connected with the conveying cylinders, and the interior of the suction filter cylinders is provided with a material taking assembly.

[0009] Furthermore, the pore size of the filter membrane located below is smaller than the pore size of the filter membrane located above, and the material taking assembly includes a fixed plate fixedly connected to the lower side of the inner part of the filter cylinder, a tympanum is fixedly connected to the inner part of the fixed plate, and two lifting rods are fixedly connected to the upper side of the tympanum, and a baffle is hingedly provided inside the filter cylinder, and the number of the baffles is two, and the upper sides of the two baffles are overlapped on the bottom end of the same conveying cylinder, and the upper sides of the two baffles are fixedly connected to the first pressure springs, and the top ends of the two first pressure springs are fixedly connected to the inner upper side of the filter cylinder.

[0010] Furthermore, a liquid storage assembly is provided inside the suction filter cylinder and inside the movable seat. The liquid storage assembly includes two connecting plates fixedly connected to the outer sides of two lifting rods.

[0011] Furthermore, the lower sides of the two connecting plates are commonly fixedly connected with a baffle bar, and the number of baffle bars is two. The lower side of the filter cartridge is provided with a through groove, and the number of through grooves is two. After the two baffle bars move downward, they are respectively inserted into the two through grooves, and the two through grooves are connected to the interior of the filter cartridge. Two storage cavities are provided on the upper side of the movable seat, and the corresponding two through grooves are connected to the same storage cavity.

[0012] Furthermore, a liquid separation component is provided inside the movable seat, and the liquid separation component includes a liquid separation cavity opened on the lower side of the two storage cavities, and the number of the liquid separation cavities is two. An insertion cavity is opened on the lower side of the movable seat, and the number of the insertion cavities is multiple, and every four insertion cavities form a group, and each group of the insertion cavities is connected to the corresponding liquid separation cavity, and the interiors of the multiple insertion cavities are all plugged with storage tubes, and the interiors of the multiple storage tubes are all plugged with first magnetic blocks, and after the first magnetic block is inserted into the interior of the storage tube, the connection between the liquid separation cavity and the insertion cavity is blocked, and the multiple The upper side of the first magnetic block is fixedly connected with a second pressure spring, the other end of the second pressure spring is fixedly connected to the inner upper side of the insertion cavity, the inner upper side of the shell is fixedly connected with a fixing frame, the lower side of the fixing frame is fixedly connected with a second magnetic block, and the number of the second magnetic blocks is multiple, and after the moving seat moves upward, the multiple second magnetic blocks are inserted into the upper side of the moving seat, and after the second magnetic block is inserted into the upper side of the moving seat, it is magnetically attracted to the upper side of the first magnetic block, and after the first magnetic block and the second magnetic block are attracted to each other, the first magnetic block slides from the storage tube into the interior of the insertion cavity.

[0013] Furthermore, a locking component is provided on the interior of the movable base and the inner back of the shell. The locking component includes a plurality of limiting grooves provided inside the movable base.

[0014] Furthermore, the inner front sides of the plurality of limit grooves are fixedly connected with tension springs, the other ends of the plurality of tension springs are fixedly connected with moving bars, and the plurality of moving bars are respectively slidably connected in the plurality of limit grooves, a card slot is provided on one side of the moving bar that is close to the storage tube, and the number of the card slots is two, the outer sides of the plurality of storage tubes are provided with grooves, and the openings of the grooves are all oriented toward the corresponding moving bars, the moving bars are slidably connected in the grooves, and the card slots are connected to the grooves after the moving bars move, the rear ends of the plurality of moving bars are commonly fixedly connected with a first magnetic strip, and the inner back side of the shell is fixedly connected with a second magnetic strip, and the first magnetic strip is magnetically adsorbed to the opposite surface of the second magnetic strip after moving downward.

[0015] Furthermore, a membrane changing assembly is provided on the upper side and the interior of the shell, and the membrane changing assembly includes a cylinder fixedly installed inside the upper side of the shell.

[0016] Furthermore, the output end of the cylinder is fixedly connected to the upper side of the moving seat, the upper side of the outer shell is fixedly connected with a cover plate, the lower side of the cover plate is fixedly connected to the upper side of the bracket, and after the moving seat moves downward, the cover plate pulls the bracket, and the inner upper side of the outer shell is fixedly connected with a guide rod, and the number of the guide rods is two, and the two guide rods are movable through the moving seat.

[0017] A method for using a microbial detection device:

[0018] S1: First, pour the sample into the delivery tube, the internal filter membrane will retain the sample, and the suction force generated by the connection between the suction pipe and the external suction pump will extract the sample above the filter membrane;

[0019] S2: The tympanic membrane inside the filter cylinder bulges and lifts the two lifting rods, driving the two baffles to open, and the suction force will also enter the interior of the conveying cylinder;

[0020] S3: The lifting rod takes out the two baffles from the inside of the through slot through the connecting plate, and the bulging tympanic membrane introduces the sample into the through slot and into the storage cavity. After the tympanic membrane falls, the baffle re-blocks the conveying cylinder, and the baffle blocks the through slot;

[0021] S4: The sample enters multiple storage tubes from the liquid separation chamber, and after the movable seat moves downward, the first magnetic block pushes and seals the storage tube. After the storage tube is inserted into the detector body, the storage tube loses the restriction from the moving bar. After the cylinder is pulled upward, the storage tube will stay inside the detector body.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The present invention extracts the sample retained by the filter membrane through the provided suction filter cartridge, and performs layered filtration with the help of different filter membranes. The suction force generated by the suction tube inside the suction filter cartridge will accelerate the sample to pass through the filter membrane, and the multi-layer filtration will effectively reduce the diversity of impurities inside the sample, so as to ensure the accuracy of the sample when it is detected by the detector body, and at the same time avoid the influence of undissolved antibiotic solutions and drugs on the sample detection data.

[0024] (2) The present invention opens the baffle plate that blocks the conveying tube by lifting the tympanic membrane suction and storing energy for the suction. The baffle plate blocks the conveying tube only after the tympanic membrane swells due to the suction. At this time, sufficient suction will be accumulated inside the filter tube to accelerate the speed of the sample passing through the two filter membranes, further improving the filtration efficiency of the undissolved antibiotic solution and drugs, and preventing the detection data of the sample from being affected.

[0025] (3) The present invention transports samples by virtue of the eardrum that is arranged to swell up and down due to suction, and timely blocks the transport tube. Since the suction tube drives the eardrum to swell up and down due to suction, the filtered sample can be guided to flow rapidly, and when the extraction stops, the storage tube will be blocked by the baffle to prevent outside air from being drawn into the filter tube and causing the sample to be contaminated by outside impurities again, thereby once again ensuring the accuracy of the sample detection data. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the present invention;

[0027] Figure 2 It is a schematic diagram of the cross-section structure of the present invention;

[0028] Figure 3 It is a schematic cross-sectional structural diagram of the suction filter cartridge of the present invention;

[0029] Figure 4 It is a schematic cross-sectional structure diagram of the outer frame of the present invention;

[0030] Figure 5 It is a schematic cross-sectional structural diagram of the mobile seat of the present invention;

[0031] Figure 6 It is a schematic cross-sectional structural diagram of the insertion cavity of the present invention;

[0032] Figure 7 is a schematic cross-sectional structural diagram of the storage cavity of the present invention;

[0033] Figure 8 It is a schematic cross-sectional structure diagram of the limiting groove of the present invention;

[0034] Fig. 9 It is a schematic cross-sectional structural diagram of the moving strip of the present invention.

[0035] Description of the numbers in the figure:

[0036] 1. Detector body; 2. Shell; 3. Slide; 4. Sliding block; 5. Moving seat; 6. Filtering component; 61. Filtering cylinder; 62. Air extraction pipe; 63. Conveying cylinder; 64. Bracket; 65. Filter membrane; 66. Material taking component; 661. Fixed plate; 662. Tympanic membrane; 663. Lifting rod; 664. Baffle; 665. First pressure spring; 67. Liquid storage component; 671. Connecting plate; 672. Baffle bar; 673. Through slot; 674. Storage chamber ; 68. Liquid separation assembly; 681. Liquid separation chamber; 682. Insertion chamber; 683. Storage tube; 684. First magnetic block; 685. Second pressure spring; 686. Fixed bracket; 687. Second magnetic block; 7. Locking component; 71. Limiting groove; 72. Tension spring; 73. Card slot; 74. Groove; 75. First magnetic strip; 76. Second magnetic strip; 77. Membrane changing assembly; 771. Cylinder; 772. Cover plate; 773. Guide rod; 78. Moving strip. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work are within the scope of protection of the present invention.

[0038] See also Figures 1 to 9A microorganism detection device comprises a detector body 1 and a shell 2 half-wrapped on the outside of the detector body 1, a slide groove 3 is provided in the middle of the front end of the shell 2, the number of the slide grooves 3 is multiple, the interiors of the multiple slide grooves 3 are all slidably connected with sliders 4, the front faces of the multiple sliders 4 are commonly fixedly connected with a moving seat 5, a suction filter component 6 is provided on the upper side of the moving seat 5, and the suction filter component 6 comprises two suction filter cartridges 61 fixedly connected to the upper side of the moving seat 5;

[0039] The outer sides of the two suction filter cylinders 61 are connected with suction pipes 62, and the other ends of the two suction pipes 62 are connected to the external suction pump. The upper outer sides of the two suction filter cylinders 61 are sleeved with conveying cylinders 63, and the interior of the conveying cylinders 63 is connected with brackets 64. The interior of the brackets 64 is overlapped with two filter membranes 65. The suction filter cylinder 61 is connected with the suction pipe 62, and the suction filter cylinder 61 is connected with the conveying cylinder 63. The interior of the suction filter cylinder 61 is provided with a material taking assembly 66.

[0040] By adopting the above technical solution, the detector body 1 is first installed inside the shell 2, and the movable seat 5 is connected to the slide groove 3 inside the shell 2 through the slider 4 and can slide up and down freely. Then, one end of the suction pipe 62 is connected to the suction filter cylinder 61, and the other end is connected to the external suction pump. When the sample enters the inside of the conveying cylinder 63, the filter membrane 65 above the bracket 64 will cause the sample to stagnate above the filter membrane 65. When the suction pipe 62 generates suction through the external suction pump, the sample above the filter membrane 65 will pass through the upper filter membrane 65 to reach the top of the lower filter membrane 65, and finally pass through The lower filter membrane 65 enters the interior of the suction filter cartridge 61, and when the sample passes through the upper filter membrane 65, the impurities in the sample will be filtered out. When passing through the second filter membrane 65, the undissolved antibiotic solution and medicines will be further filtered and finally enter the suction filter cartridge 61. The suction force generated by the vacuum tube 62 inside the suction filter cartridge 61 will accelerate the sample to pass through the filter membrane 65, and the multi-layer filtration will effectively reduce the diversity of impurities inside the sample to ensure the accuracy of the sample when it is detected by the detector body 1, while avoiding the influence of the undissolved antibiotic solution and medicines on the sample detection data.

[0041] like Figures 3 to 5 As shown, the pore size of the filter membrane 65 located at the bottom is smaller than the pore size of the filter membrane 65 located at the top, and the material taking component 66 includes a fixed plate 661 fixedly connected to the lower side of the inner part of the suction filter cylinder 61, and a tympanum 662 is fixedly connected to the inner part of the fixed plate 661, and two lifting rods 663 are fixedly connected to the upper side of the tympanum 662, and a baffle plate 664 is hingedly connected to the inner part of the suction filter cylinder 61, and the number of baffle plates 664 is two, and the upper sides of the two baffle plates 664 are overlapped on the bottom end of the same conveying cylinder 63, and the upper sides of the two baffle plates 664 are fixedly connected to the first pressure springs 665, and the top ends of the two first pressure springs 665 are fixedly connected to the inner upper side of the suction filter cylinder 61.

[0042] By adopting the above technical solution, first, after the air extraction pipe 62 generates suction force on the inside of the filter cartridge 61, the eardrum 662 inside the fixed plate 661 will bulge upward due to the suction force, and drive the two lifting rods 663 to move upward, and after the lifting rods 663 come into contact with the baffle plate 664, they will push one side of the baffle plate 664, so that the part of the baffle plate 664 that is blocked below the conveying cylinder 63 is separated from the conveying cylinder 63. At this time, the suction force inside the filter cartridge 61 will enter the conveying cylinder 63 and filter the sample, causing the sample inside the conveying cylinder 63 to enter the inside of the filter cartridge 61. After the pump stops working, the eardrum 662 will move downward due to the weight of the lifting rod 663 and the thrust of the first pressure spring 665 on the baffle 664, and the baffle 664 will block the conveying tube 63 again after being pushed by the first pressure spring 665. The baffle 664 will release the blockage of the conveying tube 63 only after the eardrum 662 swells due to the suction force. At this time, the interior of the filter tube 61 will accumulate enough suction to accelerate the speed of the sample passing through the two filter membranes 65, further improving the filtration efficiency of the undissolved antibiotic solution and drugs, and preventing the detection data of the sample from being affected.

[0043] like Figures 5 to 7 As shown, a liquid storage assembly 67 is provided inside the suction filter cartridge 61 and inside the movable seat 5 . The liquid storage assembly 67 includes two connecting plates 671 fixedly connected to the outer sides of the two lifting rods 663 .

[0044] A baffle 672 is fixedly connected to the lower sides of the two connecting plates 671, and the number of baffles 672 is two. A through groove 673 is opened on the lower side of the filter cartridge 61, and the number of through grooves 673 is two. After the two baffles 672 move downward, they are respectively inserted into the two through grooves 673, and the two through grooves 673 are connected to the interior of the filter cartridge 61. Two storage chambers 674 are opened on the upper side of the movable seat 5, and the corresponding two through grooves 673 are connected to the same storage chamber 674.

[0045] By adopting the above technical solution, first, when the eardrum 662 bulges, the lifting rod 663 will also drive the connecting plate 671 to move upward, so that the baffle 672 under the connecting plate 671 will be separated from the inside of the through groove 673, and the slope generated after the eardrum 662 bulges will guide the sample to flow into the through groove 673, and finally the sample on the filtered outside will be input into the storage chamber 674 inside the movable seat 5, and when the eardrum 662 bulges downward, the connecting plate 671 will drive the baffle 672 to enter the through groove 673 again, because the exhaust pipe 62 drives the eardrum 662 to bulge up and down through suction, the filtered sample can be guided to flow quickly, and when the extraction stops, the storage cylinder will be blocked by the baffle 664 to prevent the outside air from being drawn into the filter cylinder 61 and causing the sample to be contaminated by external impurities again, thereby once again ensuring the accuracy of the sample detection data.

[0046] like Figure 7 As shown, a liquid separation component 68 is provided inside the movable seat 5, and the liquid separation component 68 includes a liquid separation cavity 681 opened on the lower side of the two storage cavities 674, and the number of the liquid separation cavities 681 is two, and an insertion cavity 682 is opened on the lower side of the movable seat 5, and the number of the insertion cavities 682 is multiple, and every four insertion cavities 682 form a group, and each group of insertion cavities 682 is connected to the corresponding liquid separation cavity 681, and the interiors of the multiple insertion cavities 682 are all plugged with storage tubes 683, and the interiors of the multiple storage tubes 683 are all plugged with first magnetic blocks 684, and after the first magnetic block 684 is inserted into the interior of the storage tube 683, the connection between the liquid separation cavity 681 and the insertion cavity 682 is blocked, and the multiple first The upper side of the magnetic block 684 is fixedly connected with a second pressure spring 685, and the other end of the second pressure spring 685 is fixedly connected to the inner upper side of the insertion cavity 682. The inner upper side of the outer shell 2 is fixedly connected with a fixing frame 686, and the lower side of the fixing frame 686 is fixedly connected with a second magnetic block 687, and the number of the second magnetic blocks 687 is multiple. After the movable seat 5 moves upward, the multiple second magnetic blocks 687 are inserted into the upper side of the movable seat 5. After the second magnetic block 687 is inserted into the upper side of the movable seat 5, it is magnetically attracted to the upper side of the first magnetic block 684. After the first magnetic block 684 and the second magnetic block 687 are attracted to each other, the first magnetic block 684 slides from the storage tube 683 into the interior of the insertion cavity 682.

[0047] By adopting the above technical solution, first, after the sample enters the storage chamber 674, the liquid separation chamber 681 inside the storage chamber 674 will guide the sample to the multiple insertion chambers 682. At this time, the upper part of the movable seat 5 is in contact with the fixed frame 686, and the second magnetic block 687 below the fixed frame 686 is inserted into the upper part of the movable seat 5, so that the second magnetic block 687 adsorbs the first magnetic block 684 inside the insertion chamber 682, so that the first magnetic block 684 squeezes the second pressure spring 685, and the sample will be collected by the storage tube 683 inside the insertion chamber 682. In the process of the movable seat 5 moving downward, the second magnetic block 687 is gradually Gradually move away from the first magnetic block 684. At this time, the second pressure spring 685 will push the first magnetic block 684 to insert it into the storage tube 683, and block the connection between the insertion cavity 682 and the storage cavity 674. At the same time, the entrance of the storage tube 683 will also be blocked. Since the storage tube 683 can be taken out from under the movable seat 5, the storage tube 683 is blocked after the sample is introduced into the storage tube 683, which can ensure the cleanliness of the test sample. At the same time, the same batch of samples can be divided into multiple groups for synchronous testing, avoiding errors in sample test data due to differences in regional distribution of microorganisms inside the sample.

[0048] like Figure 8 and Fig. 9 As shown, a locking component 7 is provided inside the movable base 5 and on the inner back side of the housing 2 . The locking component 7 includes a plurality of limiting grooves 71 formed inside the movable base 5 .

[0049] The inner front sides of the plurality of limit grooves 71 are fixedly connected with tension springs 72, the other ends of the plurality of tension springs 72 are fixedly connected with moving bars 78, and the plurality of moving bars 78 are respectively slidably connected in the plurality of limit grooves 71, a slot 73 is provided on one side of the moving bar 78 that is close to the storage tube 683, and the number of the slots 73 is two, the outer sides of the plurality of storage tubes 683 are provided with grooves 74, and the openings of the grooves 74 are all oriented toward the corresponding moving bars 78, the moving bars 78 are slidably connected in the grooves 74, and the slots 73 are connected to the grooves 74 after the moving bars 78 move, the rear ends of the plurality of moving bars 78 are commonly fixedly connected with a first magnetic strip 75, and the inner back side of the shell 2 is fixedly connected with a second magnetic strip 76, and after the first magnetic strip 75 moves downward, it is magnetically adsorbed to the opposite surface of the second magnetic strip 76.

[0050] By adopting the above technical solution, first, when the moving seat 5 moves downward, the moving seat 5 will drive the storage tube 683 to insert into the detection cavity at the corresponding position of the detector body 1. After the detection is completed, the moving bar 78 in the internal limit groove 71 of the moving seat 5 is fixed to the same first magnetic strip 75, and after the moving seat 5 and the detector body 1 are attached, the first magnetic strip 75 will correspond to the position of the second magnet, and the two are magnetically attracted, so that the first magnetic strip 75 pulls multiple moving bars 78 at the same time, and the card slot 73 opened on the side of the moving bar 78 and the storage tube 6 After the groove 74 on the outside of 83 corresponds, the moving bar 78 will lose the restriction on the storage tube 683. During the upward movement of the moving seat 5, the storage tube 683 will stay above the detector body 1. After the first magnetic strip 75 and the second magnetic strip 76 move away from each other, the tension spring 72 inside the limit groove 71 will pull the moving bar 78 to reset it. Since the storage tube 683 that collects multiple groups of samples will stay above the detector body 1 after the detection of the detector body 1 is completed, it is convenient to uniformly process the samples after the detection is completed, and at the same time, it also ensures the detection efficiency of subsequent samples.

[0051] like Figure 4 As shown, a membrane changing assembly 77 is provided on the upper side and the interior of the housing 2 . The membrane changing assembly 77 includes a cylinder 771 fixedly installed inside the upper side of the housing 2 .

[0052] The output end of the cylinder 771 is fixedly connected to the upper side of the moving seat 5, the upper side of the outer shell 2 is fixedly connected with a cover plate 772, the lower side of the cover plate 772 is fixedly connected to the upper side of the bracket 64, and after the moving seat 5 moves downward, the cover plate 772 pulls the bracket 64, and the inner upper side of the outer shell 2 is fixedly connected with a guide rod 773, and there are two guide rods 773, and both guide rods 773 are movable and penetrate the moving seat 5.

[0053] By adopting the above technical solution, the movable seat 5 is first pushed downward by the cylinder 771, and the movable seat 5 will always be on the outside of the two guide rods 773 during the downward movement. Since the upper part of the outer shell 2 is fixed to the cover plate 772, and the lower part of the cover plate 772 is fixed to the bracket 64, when the movable seat 5 drives the filter cylinder 61 to move downward, the bracket 64 and the filter membrane 65 inside it will be exposed from the inside of the conveying cylinder 63. When the movable seat 5 is at the upper part, the filter membrane 65 can filter the sample, and after the movable seat 5 moves downward to detect the sample, the filter membrane 65 will be directly exposed to facilitate the rapid replacement and extraction of the filter membrane 65, thereby further improving the detection efficiency of the sample.

[0054] A method for using a microbial detection device:

[0055] S1: First, the sample is poured into the conveying tube 63, and the internal filter membrane 65 retains the sample. The suction force generated by the air suction pipe 62 connected to the external air suction pump extracts the sample above the filter membrane 65;

[0056] S2: The tympanic membrane 662 inside the suction cylinder 61 swells and lifts the two lifting rods 663, driving the two baffles 664 to be opened, and the suction force will also enter the interior of the conveying cylinder 63;

[0057] S3: The lifting rod 663 removes the two baffles 672 from the through slot 673 through the connecting plate 671, and the bulging eardrum 662 introduces the sample into the through slot 673 and into the storage chamber 674. After the eardrum 662 falls, the baffle 664 blocks the conveying cylinder 63 again, and the baffle 672 blocks the through slot 673;

[0058] S4: The sample enters into the multiple storage tubes 683 from the liquid separation chamber 681, and after the movable seat 5 moves downward, the first magnetic block 684 pushes and seals the storage tubes 683. After the storage tubes 683 are inserted into the detector body 1, the storage tubes 683 lose the restriction from the moving bar 78. After the cylinder 771 is pulled upward, the storage tubes 683 will stay inside the detector body 1.

[0059] Working principle: First, install the detector body 1 inside the housing 2, and one end of the air extraction pipe 62 is connected to the filter cartridge 61, and the other end is connected to the external air extraction pump. After the sample passes through the cover plate 772 and enters the inside of the delivery cylinder 63, the air extraction pipe 62 generates suction through the external air extraction pump, so that the sample above the filter membrane 65 will pass through the upper filter membrane 65 to the top of the lower filter membrane 65, and finally pass through the lower filter membrane 65 to enter the filter cartridge 61, and finally enter the filter. Before this, the tympanic membrane 662 inside the fixed plate 661 will swell upward due to the suction force, and drive the two lifting rods 663 to move upward and push the baffle 664, so that the baffle 664 is separated from the bottom of the conveying cylinder 63. At this time, the suction force inside the filter cylinder 61 will enter the inside of the conveying cylinder 63. After the external vacuum pump stops working, the tympanic membrane 662 will move downward due to the weight of the lifting rod 663 and the thrust of the first pressure spring 665 on the baffle 664. , and the baffle plate 664 will be pushed by the first pressure spring 665 to block the conveying cylinder 63 again, and while the tympanic membrane 662 bulges, the lifting rod 663 will also drive the connecting plate 671 and the baffle bar 672 below it to move upward, and after the baffle bar 672 is separated from the inside of the through groove 673, the slope generated by the bulging of the tympanic membrane 662 will guide the sample to flow into the through groove 673, and finally enter the storage chamber 674 inside the movable seat 5, and when the tympanic membrane 662 bulges downward, the connecting plate 671 will drive the baffle bar 672 to enter the through groove 673 again, and the sample entering the storage chamber 674 will be guided to the multiple insertion chambers 682 through the liquid separation chamber 681, at this time, the upper part of the movable seat 5 is in contact with the fixed frame 686 and the second magnetic block 687 below it is inserted into the upper part of the movable seat 5, and the first magnetic block 684 inside the insertion chamber 682 is adsorbed, and the first magnetic block 684 is pulled upward, and the sample will be collected by the storage tube 683 inside the insertion chamber 682;

[0060] When the movable seat 5 is pushed downward by the cylinder 771, the second magnetic block 687 gradually moves away from the first magnetic block 684. At this time, the second pressure spring 685 will push the first magnetic block 684 to insert it into the storage tube 683, and block the connection between the insertion cavity 682 and the storage cavity 674. At the same time, the entrance of the storage tube 683 will also be blocked. After the movable seat 5 drives the storage tube 683 to insert into the detection cavity at the corresponding position of the detector body 1 and completes the detection, the movable strip 78 in the internal limiting groove 71 of the movable seat 5 is fixed to the same first magnetic strip 75, and after the movable seat 5 is attached to the detector body 1, the first magnetic strip 75 will correspond to the position of the second magnet, and the magnetic adsorption between the two makes the first magnetic strip 75 simultaneously move multiple movable strips 78. 8 is pulled, and after the slot 73 opened on the side of the moving bar 78 corresponds to the groove 74 on the outside of the storage tube 683, the moving bar 78 will lose the restriction on the storage tube 683, and the storage tube 683 will stay above the detector body 1 during the upward movement of the moving seat 5, and after the first magnetic strip 75 and the second magnet are separated, the tension spring 72 inside the limit groove 71 will pull the moving bar 78 and the first magnetic strip 75. At the same time, because the moving seat 5 will always be on the outside of the two guide rods 773 during the downward movement, and the upper part of the shell 2 is fixed to the cover plate 772, and the lower part of the cover plate 772 is fixed to the bracket 64, when the moving seat 5 drives the filter cartridge 61 to move downward, the bracket 64 and the filter membrane 65 inside it will be exposed from the inside of the conveying cylinder 63.

[0061] The above is only a preferred specific implementation of the present invention; however, the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and its improved conception within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A microorganism detection device, comprising a detector body (1) and a shell (2) half-wrapped outside the detector body (1), a slide groove (3) is provided in the middle of the front end of the shell (2), the slide grooves (3) are multiple in number, a slider (4) is slidably connected inside the multiple slide grooves (3), and a movable seat (5) is fixedly connected to the front of the multiple sliders (4), characterized in that: A suction filter component (6) is provided on the upper side of the movable seat (5), and the suction filter component (6) comprises two suction filter cartridges (61) fixedly connected to the upper side of the movable seat (5); The outer sides of the two suction filter cylinders (61) are both connected with suction pipes (62), and the other ends of the two suction pipes (62) are both connected with an external suction pump. The upper outer sides of the two suction filter cylinders (61) are both sleeved with a conveying cylinder (63), and the interior of the conveying cylinder (63) is connected with a bracket (64), and the interior of the bracket (64) is overlapped with two filter membranes (65). The suction filter cylinder (61) is connected with the suction pipe (62), and the suction filter cylinder (61) is connected with the conveying cylinder (63). A material taking component (66) is provided inside the suction filter cylinder (61).

2. A microorganism detection device according to claim 1, characterized in that: The aperture of the filter membrane (65) located at the bottom is smaller than the aperture of the filter membrane (65) located at the top. The material taking component (66) includes a fixed plate (661) fixedly connected to the lower side of the suction filter cylinder (61). The fixed plate (661) is fixedly connected to the inside of a tympanic membrane (662). The upper side of the tympanic membrane (662) is fixedly connected to two lifting rods (663). The interior of the suction filter cylinder (61) is hinged with a baffle (664). There are two baffles (664). The upper sides of the two baffles (664) are overlapped on the bottom end of the same conveying cylinder (63). The upper sides of the two baffles (664) are fixedly connected to a first pressure spring (665). The top ends of the two first pressure springs (665) are fixedly connected to the upper side of the suction filter cylinder (61).

3. A microorganism detection device according to claim 2, characterized in that: A liquid storage assembly (67) is provided inside the suction filter cylinder (61) and inside the movable seat (5). The liquid storage assembly (67) includes two connecting plates (671) fixedly connected to the outside of the two lifting rods (663).

4. A microorganism detection device according to claim 3, characterized in that: A baffle (672) is fixedly connected to the lower sides of the two connecting plates (671), and the number of baffles (672) is two. A through groove (673) is provided on the lower side of the suction filter cartridge (61), and the number of through grooves (673) is two. After the two baffles (672) move downward, they are respectively inserted into the two through grooves (673), and the two through grooves (673) are both connected to the interior of the suction filter cartridge (61). Two storage cavities (674) are provided on the upper side of the movable seat (5), and the corresponding two through grooves (673) are connected to the same storage cavity (674).

5. A microorganism detection device according to claim 1, characterized in that: The movable seat (5) is provided with a liquid separation component (68) inside, and the liquid separation component (68) includes a liquid separation cavity (681) opened on the lower side of the two storage cavities (674), and the number of the liquid separation cavities (681) is two. The lower side of the movable seat (5) is provided with an insertion cavity (682), and the number of the insertion cavities (682) is multiple, and every four of the insertion cavities (682) form a group, and each group of the insertion cavities (682) is connected to the corresponding liquid separation cavity (681), and the interiors of the multiple insertion cavities (682) are all plugged with storage tubes (683), and the interiors of the multiple storage tubes (683) are all plugged with first magnetic blocks (684), and after the first magnetic block (684) is inserted into the interior of the storage tube (683), the connection between the liquid separation cavity (681) and the insertion cavity (682) is blocked, and the multiple insertion cavities (682) are connected to the storage tube (683). The upper side of the first magnetic block (684) is fixedly connected with a second pressure spring (685), and the other end of the second pressure spring (685) is fixedly connected to the inner upper side of the insertion cavity (682). The inner upper side of the shell (2) is fixedly connected with a fixing frame (686), and the lower side of the fixing frame (686) is fixedly connected with a second magnetic block (687), and the number of the second magnetic blocks (687) is multiple. After the movable seat (5) moves upward, the multiple second magnetic blocks (687) are inserted into the upper side of the movable seat (5). After the second magnetic block (687) is inserted into the upper side of the movable seat (5), it is magnetically attracted to the upper side of the first magnetic block (684). After the first magnetic block (684) and the second magnetic block (687) are attracted to each other, the first magnetic block (684) slides from the storage tube (683) into the interior of the insertion cavity (682).

6. A microorganism detection device according to claim 1, characterized in that: The interior of the movable seat (5) and the inner back surface of the outer shell (2) are both provided with a locking component (7), and the locking component (7) comprises a plurality of limiting grooves (71) provided inside the movable seat (5).

7. A microorganism detection device according to claim 6, characterized in that: The inner front sides of the plurality of limit grooves (71) are all fixedly connected with tension springs (72), the other ends of the plurality of tension springs (72) are all fixedly connected with moving bars (78), and the plurality of moving bars (78) are respectively slidably connected in the plurality of limit grooves (71), a card slot (73) is provided on one side of the moving bar (78) that is in contact with the storage tube (683), and the number of the card slots (73) is two, and the outer sides of the plurality of storage tubes (683) are all provided with grooves (74), and The openings of the grooves (74) are all oriented toward the corresponding moving strips (78), the moving strips (78) are slidably connected in the grooves (74), the card slots (73) are connected to the grooves (74) after the moving strips (78) move, the rear ends of the plurality of moving strips (78) are commonly fixedly connected to a first magnetic strip (75), the front end of the housing (2) is fixedly connected to a second magnetic strip (76), and the first magnetic strip (75) is magnetically adsorbed to the opposite surface of the second magnetic strip (76) after moving downward.

8. A microorganism detection device according to claim 1, characterized in that: A membrane replacement assembly (77) is provided on the upper side and inside of the outer shell (2). The membrane replacement assembly (77) comprises a cylinder (771) fixedly mounted inside the upper side of the outer shell (2).

9. A microorganism detection device according to claim 8, characterized in that: The output end of the cylinder (771) is fixedly connected to the upper side of the movable seat (5); the upper side of the outer shell (2) is fixedly connected to a cover plate (772); the lower side of the cover plate (772) is fixedly connected to the upper side of the bracket (64); after the movable seat (5) moves downward, the cover plate (772) pulls the bracket (64); the inner upper side of the outer shell (2) is fixedly connected to a guide rod (773), and the number of the guide rods (773) is two, and both guide rods (773) movably penetrate the movable seat (5).

10. A method for using a microorganism detection device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: First, the sample is poured into the conveying tube (63), and the filter membrane (65) inside the conveying tube retains the sample. The suction force generated by the connection between the suction pipe (62) and the external suction pump extracts the sample above the filter membrane (65); S2: The tympanic membrane (662) inside the suction cylinder (61) swells and lifts the two lifting rods (663), driving the two baffles (664) to be opened, and the suction force will also enter the interior of the conveying cylinder (63); S3: The lifting rod (663) removes the two blocking bars (672) from the through slot (673) through the connecting plate (671), and the bulging eardrum (662) introduces the sample into the through slot (673) and into the storage chamber (674). After the eardrum (662) falls, the blocking plate (664) blocks the conveying cylinder (63) again, and the blocking bars (672) block the through slot (673); S4: The sample enters the interior of multiple storage tubes (683) from the liquid separation chamber (681), and after the movable seat (5) moves downward, the first magnetic block (684) pushes and seals the storage tube (683). After the storage tube (683) is inserted into the interior of the detector body (1), the storage tube (683) loses the restriction from the moving bar (78), and after the cylinder (771) is pulled upward, the storage tube (683) will stay inside the detector body (1).

Images