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Strain of Acidithiobacillus ferrooxidans and its application in environmental treatment

A technology of Thiobacillus ferrooxidans and environmental treatment, which is applied in the direction of bacteria, biological sludge treatment, chemical instruments and methods, etc., can solve the problems of black and odorous water and sediment treatment difficulties, and achieve rapid deblackening and deodorization, Achieve ecological restoration and change the effect of surface activity

Active Publication Date: 2018-05-11
武汉新禹智水环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of acidophilic Thiobacillus ferrooxidans for the problem of black and odorous water and sediment treatment in the environment, which has been preserved in the China Typical Culture Collection Center on December 19, 2017 Classification name: Acidithiobacillus ferrooxidans DY-1, deposit number: CCTCC NO: M2017806, hereinafter referred to as Acidithiobacillus ferrooxidans DY-1, address: Wuhan University, Wuhan, China

Method used

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  • Strain of Acidithiobacillus ferrooxidans and its application in environmental treatment
  • Strain of Acidithiobacillus ferrooxidans and its application in environmental treatment
  • Strain of Acidithiobacillus ferrooxidans and its application in environmental treatment

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Embodiment 1

[0034] Obtaining of acidophilus Thiobacillus ferrooxidans DY-1 strain.

[0035] The samples were collected from the slag of a certain mine in Daye, Hubei Province, and after repeated enrichment and purification, single colony strains were obtained and identified.

[0036] The separation and purification process of the acidic Thiobacillus ferrooxidans DY-1 strain: take 5 g of slag samples and dissolve them in 50 mL of sterile water, shake and culture at 28° C. and 180 r / min for 6 h, and then let stand for 2 h. Take 10mL of the water sample containing the bacteria in the upper layer and add it to 100mL of sterilized 9K liquid medium, and cultivate it on a shaker at 28°C and 180r / min until the medium changes from light green to reddish brown, and then carry out 5 enrichment cultures , after the time until the reddish-brown color appeared changed from 5d to 1d, the bacteria were isolated and purified by the solid medium coating plate method to obtain pure cultured single strains. ...

Embodiment 2

[0040] The properties of acidophilus Thiobacillus ferrooxidans DY-1:

[0041] 1. Morphological identification, including the observation and description of colony morphology and microscopic examination records after Gram staining. The characteristics are as follows: cultured on solid medium for 7 days at 28°C, small and convex circular single colonies are formed, the cells are bright yellow, with small black protrusions in the center, and the edges of the colonies are regular ( figure 1 ); Gram staining, microscopic examination was Gram-negative. Observed under the microscope, it is usually distributed in a single, two or several chains. A single bacterium is a brevibacterium with a length of about 1-1.5 μm and a diameter of about 0.5 μm. figure 2 ).

[0042] 2. 16S rDNA molecular identification, that is, using prokaryotic 16S rDNA universal primers 27F and 1492R (27F sequence: -AGAGTTTGATCCTGGCTCAG-, 1492R sequence: -GGTTACCTTGTTACGACTT-) for PCR sequence amplification. A...

Embodiment 3

[0047] Application of acidophilus Thiobacillus ferrooxidans DY-1 in deblackening and deodorizing black and odorous water.

[0048] The black and smelly water samples used in the experiment were collected from a black and smelly river in Wuhan. The experimental device is a cylindrical container with an inner diameter of 10 cm and a height of 35 cm, filled with 1 L of black and odorous water samples. The experiment is divided into experimental group and control group. The experimental group was injected with 10% (volume ratio) activated DY-1 bacterial suspension, and the control group was injected with the same amount of sterilized distilled water. Each group was treated with 3 replicates, all placed at 28°C, aerated and oxygenated. Water samples were taken at 12h, 24h, 48h, and 72h for Fe 2+ The oxidation rate and S 2- , organic matter, ammonia nitrogen removal rate analysis, the results are shown in Table 2.

[0049] Fe 2+ The oxidation rate can reflect the activity of s...

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Abstract

The invention discloses a strain of Acidithiobacillus ferrooxidans and its application in environmental treatment. The Acidithiobacillus ferrooxidans is Acidithiobacillus ferrooxidans DY-1 and has a preservation number of CCTCC NO: M2017806. Through good biological oxidation, bio-acidic effect, strong metal tolerance and metal adsorption capacity, the Acidithiobacillus ferrooxidans DY-1 can produce significant effects in decoloration and deodorization of black and odor water and ecological restoration of sediments and does not damage the existing water system. The Acidithiobacillus ferrooxidans DY-1 can be used as a vanguard for the treatment on severely polluted water and lay a good water quality and ecological micro-environment foundation for water quality improvement and water ecological balance of the later treatment methods using other microorganisms and aquatic plants.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of polluted environment, and in particular relates to an acidophilic Thiobacillus ferrooxidans and its application in environmental treatment. technical background [0002] In recent years, the deterioration of the water environment has been particularly prominent. A series of problems such as general eutrophication of lakes, frequent outbreaks of blue-green algae, river siltation, functional attenuation, black and odorous water bodies, and heavy metal pollution have not only damaged the living environment of the people, but also seriously affected the city. image. With the improvement of living standards, people's expectations for the improvement of environmental governance are getting higher and higher. Environmental protection is facing a new normal where opportunities and challenges coexist, and water environment governance has also been raised to an unprecedented level. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F11/02C12R1/01C02F101/20C02F101/22
CPCC02F3/34C02F11/02C02F2101/20C02F2101/22C12N1/205C12R2001/01
Inventor 陈正军刘陈飞孙孟源李凌峰刘畅王子谦赵永顺雷静单梅王大正
Owner 武汉新禹智水环保科技有限公司
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