A strain of acidophilus Thiobacillus ferrooxidans and its application in environmental management

A technology of Thiobacillus ferrooxidans and environmental treatment, which is applied in the direction of bacteria, biological sludge treatment, chemical instruments and methods, etc., can solve the problems of black and odorous water and sediment treatment difficulties, and achieve the improvement of water quality and strong metal resistance. Acceptability and low cost effect

Active Publication Date: 2021-07-13
武汉新禹智水环保科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a strain of acidophilic Thiobacillus ferrooxidans for the problem of black and odorous water and sediment treatment in the environment, which has been preserved in the China Typical Culture Collection Center on December 19, 2017 Classification name: Acidithiobacillus ferrooxidans DY-1, deposit number: CCTCC NO: M2017806, hereinafter referred to as Acidithiobacillus ferrooxidans DY-1, address: Wuhan University, Wuhan, China

Method used

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  • A strain of acidophilus Thiobacillus ferrooxidans and its application in environmental management
  • A strain of acidophilus Thiobacillus ferrooxidans and its application in environmental management
  • A strain of acidophilus Thiobacillus ferrooxidans and its application in environmental management

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Experimental program
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Effect test

Embodiment 1

[0035] Obtaining of acidophilus Thiobacillus ferrooxidans DY-1 strain.

[0036] The samples were collected from the slag of a certain mine in Daye, Hubei Province, and after repeated enrichment and purification, single colony strains were obtained and identified.

[0037] The separation and purification process of the acidic Thiobacillus ferrooxidans DY-1 strain: take 5 g of slag samples and dissolve them in 50 mL of sterile water, shake and culture at 28° C. and 180 r / min for 6 h, and then let stand for 2 h. Take 10mL of the water sample containing the bacteria in the upper layer and add it to 100mL of sterilized 9K liquid medium, and cultivate it on a shaker at 28°C and 180r / min until the medium changes from light green to reddish brown, and then carry out 5 enrichment cultures , after the time until the reddish-brown color appeared changed from 5d to 1d, the bacteria were isolated and purified by the solid medium coating plate method to obtain pure cultured single strains. ...

Embodiment 2

[0041] The properties of acidophilus Thiobacillus ferrooxidans DY-1:

[0042] 1. Morphological identification, including the observation and description of colony morphology and microscopic examination records after Gram staining. The characteristics are as follows: cultured on solid medium for 7 days at 28°C, small and convex circular single colonies are formed, the cells are bright yellow, with small black protrusions in the center, and the edges of the colonies are regular ( figure 1 ); Gram staining, microscopic examination was Gram-negative. Observed under the microscope, it is usually distributed in a single, two or several chains. A single bacterium is a brevibacterium with a length of about 1-1.5 μm and a diameter of about 0.5 μm. figure 2 ).

[0043] 2. 16S rDNA molecular identification, that is, using prokaryotic 16S rDNA universal primers 27F and 1492R (27F sequence: -AGAGTTTGATCCTGGCTCAG-, 1492R sequence: -GGTTACCTTGTTACGACTT-) for PCR sequence amplification. A...

Embodiment 3

[0048] Application of acidophilus Thiobacillus ferrooxidans DY-1 in deblackening and deodorizing black and odorous water.

[0049] The black and smelly water samples used in the experiment were collected from a black and smelly river in Wuhan. The experimental device is a cylindrical container with an inner diameter of 10 cm and a height of 35 cm, filled with 1 L of black and odorous water samples. The experiment is divided into experimental group and control group. The experimental group was injected with 10% (volume ratio) activated DY-1 bacterial suspension, and the control group was injected with the same amount of sterilized distilled water. Each group was treated with 3 replicates, all placed at 28°C, aerated and oxygenated. Water samples were taken at 12h, 24h, 48h, and 72h for Fe 2+ The oxidation rate and S 2- , organic matter, ammonia nitrogen removal rate analysis, the results are shown in Table 2.

[0050] Fe 2+ The oxidation rate can reflect the activity of s...

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Abstract

The invention discloses a strain of acidophilic Thiobacillus ferrooxidans and its application in environmental management. The acidophilic Thiobacillus ferrooxidans is acidophilic Thiobacillus ferrooxidans ( Acidithiobacillus ferrooxidans ) DY‑1, the preservation number is: CCTCC NO: M2017806, with good biological oxidation, biological acidity, strong metal tolerance, metal adsorption capacity, etc., acidophilic Thiobacillus ferrooxidans DY‑1 in black Remarkable results have been achieved in the de-blackening and deodorization of odorous water bodies and the ecological restoration of sediment, and will not cause damage to the original water system. It can be used as a pioneer in the treatment of heavily polluted water bodies, laying a good foundation for water quality and ecological microenvironment for subsequent use of other treatment methods such as microorganisms and aquatic plants to further improve water quality and restore water ecological balance.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of polluted environment, and in particular relates to an acidophilic Thiobacillus ferrooxidans and its application in environmental treatment. [0002] technical background [0003] In recent years, the deterioration of the water environment has been particularly prominent. A series of problems such as general eutrophication of lakes, frequent outbreaks of blue-green algae, river siltation, functional attenuation, black and odorous water bodies, and heavy metal pollution have not only damaged the living environment of the people, but also seriously affected the city. image. With the improvement of living standards, people's expectations for the improvement of environmental governance are getting higher and higher. Environmental protection is facing a new normal where opportunities and challenges coexist, and water environment governance has also been raised to an unprecedented level. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F11/02C12R1/01C02F101/20C02F101/22
CPCC02F3/34C02F11/02C02F2101/20C02F2101/22C12N1/205C12R2001/01
Inventor 陈正军刘陈飞孙孟源李凌峰刘畅王子谦赵永顺雷静单梅王大正
Owner 武汉新禹智水环保科技有限公司
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