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Acidithiobacillus ferrooxidans and applications thereof

A technology of Thiobacillus ferrooxidans and strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problem of few types of microorganisms, and achieve the effects of high leaching rate, strong activity, and high leaching efficiency

Active Publication Date: 2013-08-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, the types of microorganisms suitable for bioleaching are still relatively small
On the other hand, the metal leaching efficiency and leaching rate of these bacteria from waste PCBs need to be improved. Therefore, finding a highly efficient PCBs leaching bacteria that can truly adapt to the environment is a hot issue in the field of bioleaching.

Method used

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  • Acidithiobacillus ferrooxidans and applications thereof
  • Acidithiobacillus ferrooxidans and applications thereof
  • Acidithiobacillus ferrooxidans and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Screening and Isolation of Strain Z1

[0032] Take 40mL of raw water and inoculate into 200mL of 9K liquid culture based on 500ml Erlenmeyer flask, shake and cultivate in a constant temperature shaker at 30°C at 120rpm for 5-8d, then use 0.45μm organic microporous filter to remove the reddish-brown precipitate in the solution, and then Centrifuge the obtained filtrate in a high-speed centrifuge at 10,000rpm for 20min, collect the milky white precipitate at the bottom, which is the bacterial aggregate, and finally dilute it with deionized water to obtain a bacterial suspension, which is the mixed culture of acidophilic bacteria (MCAB). The MCAB continued to be enriched for 3 to 4 generations, and then the fresh bacterial solution cultivated to the logarithmic phase was diluted to 10 with sterile water with a pH of 2.5 on the aseptic operation bench. -5 , 10 -6 , 10 -7 Add 0.1mL of diluted bacterial solution to each TSM I solid medium plate with a diameter of 12cm, spre...

Embodiment 2

[0034] Application and effect of strain Z1 in recycling valuable metals from waste circuit boards

[0035]In order to efficiently bioleach MC powder by strain Z1, the isolated strain must first adapt to the toxicity of metals in MC powder, so it is domesticated with MC powder. The domestication method is as follows: inoculate 20mL of bacterial suspension into 200mL of sterile 9K medium, shake culture at 30°C and 160rpm, add 5.00g / L MC powder when it is in the logarithmic growth phase, and measure the pH value of the leachate After the pH began to drop and tended to be stable, 1 mL of the culture was inoculated into a new 9K medium and 7.00 g / L of metal-enriched substances were added. According to this, gradually increase the dosage of MC powder to 9.00g / L, 11.00g / L, 13.00g / L and 15.00g / L. The final acclimatization product was harvested and prepared into a bacterial suspension. Described 9K liquid culture medium is (NH 4 ) 2 SO 4 , 3.0g; KCl, 0.1g; K 2 HPO 4 , 0.5g; MgSO...

Embodiment 3

[0045] Application of initial pH value in leaching MC of strain Z1

[0046] The initial pH levels for this example were: 1.75, 2.00, 2.25, 2.50. Inoculate 90mL9K liquid culture solution into 250mL Erlenmeyer flask with 10% inoculum amount of bacterial strain Z1, and the initial Fe 2+ With a concentration of 9.00g / L, add 0.80g (8.00g / L) of MC powder with a particle size below 40-60 mesh, and place it in a constant temperature shaker at 30°C and 160rpm for shaking culture.

[0047] Instance results such as Figure 4 as shown, Figure 4 It reflects the effect of Z1 leaching MC powder under different initial pH conditions. It can be clearly seen from the figure that the leaching effect is the best when the initial pH is 2.25, which is consistent with the optimum pH for bacterial growth. Under the condition of initial pH1.75, the leaching rate of Cu increases significantly after a lag period of about 60h. It can be seen that the pH is too low or too high is not conducive to le...

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Abstract

The present invention discloses a strain of Acidithiobacillus ferrooxidans, and applications thereof. The Acidithiobacillus ferrooxidans is Acidithiobacillus ferrooxidans Z1, and is preserved in China Center for Type Culture Collection (short for CCTCC) on March 25, 2013, wherein a preservation number is CCTCC NO:M2013102. With the Acidithiobacillus ferrooxidans, valuable metals in waste PCB can be leached under an aerobic condition. The Acidithiobacillus ferrooxidans has characteristics of efficient bio-leaching, efficient leaching rate, and good environment adaptability.

Description

technical field [0001] The invention belongs to the field of solid waste biological treatment and resource utilization, and in particular relates to a Thiobacillus ferrooxidans and its application in recycling valuable metals in waste circuit boards. Background technique [0002] Discarded printed circuit boards are mainly defective products, leftovers and PCBs substrates of disassembled and assembled components of discarded electronic products produced during the manufacturing process of printed circuit boards (PCBs). Its composition is complex, containing about 30% plastic, 30% inert oxide and 40% metal. It not only contains a large amount of base metals, but also contains a certain amount of precious metals. As the production of waste PCBs increases year by year, the pollution of soil, water and air is becoming more and more serious. Therefore, the recycling of PCBs has obvious environmental significance and certain economic benefits. [0003] At present, the relatively...

Claims

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Application Information

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IPC IPC(8): C22B3/18C12N1/20C12R1/01
CPCY02P10/20
Inventor 朱能武张婷许治国吴平霄
Owner SOUTH CHINA UNIV OF TECH
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