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Acidithiobacillus and application thereof

A technology of Thiobacillus and Thiobacillus ferrooxidans, which is applied in the direction of bacteria and process efficiency improvement, can solve the problems of bacterial leaching technology constraints, and achieve the effect of strong molybdenum resistance

Active Publication Date: 2011-09-07
BEIJING RESEARCH INSTITUTE OF CHEMICAL ENGINEERING AND METALLURGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limit tolerance concentration of leaching bacteria to molybdenum is 0.16g / L -1 , which restricts the application of bacterial leaching technology in the leaching of uranium-molybdenum ore and pure molybdenum ore

Method used

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  • Acidithiobacillus and application thereof
  • Acidithiobacillus and application thereof
  • Acidithiobacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Use sterile 9K medium, with Fe 2+ For energy, the temperature is 28°C, the inoculum amount of SWLM1 strain is 10%, and the shaker culture is carried out. Sampling at 0h, 5h, 10h, 12h, 15h, 18h, 20h, 22h, 25h, 28h, 30h, 32h, 35h and 40h to measure OD 420 , with non-inoculated 9K medium as a control.

[0024] The test results showed that the SWLM1 strain remained in the lag phase within 20 hours, and the number of bacteria increased slightly, but the change was not significant. After 20h, it began to enter the logarithmic phase, and the number of bacteria increased rapidly. After 30h, the stable phase was reached, and the number of bacteria remained at the maximum state.

Embodiment 2

[0026] Use sterile 9K medium, with Fe 2+ For energy, the inoculum amount of the strain was 10%, and the shaker culture was carried out at the same time at a temperature ranging from 25 to 40°C, and the uninoculated 9K medium was used as a control.

[0027] The test results showed that 28~32℃ was the suitable growth temperature for SWLM1 strain, and 30℃ was the most suitable growth temperature for SWLM1 strain. The culture temperature lower than 28℃ significantly inhibited the growth of SWLM1 strain; the culture temperature higher than 32℃ also inhibited the growth of bacteria.

Embodiment 3

[0029] Use sterile 9K medium, with Fe 2+For energy, the temperature is 30°C, the inoculum amount of the strain is 10%, and the shaker culture is carried out at the same time in the range of pH 0.0-3.0, and the non-inoculated 9K medium is used as a control.

[0030] The test results showed that the SWLM1 strain could grow at pH 1.0-3.0, and the optimum growth pH was 2-2.5. When the pH is lower than 1, the growth of the bacteria almost stagnates; when the pH is higher than 2.5, the growth of the bacteria is also inhibited.

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Abstract

The invention relates to an acidithiobacillus and application thereof. The preserving number of acidithiobacillus ferrooxidans SWLM1 of the acidithiobacillus is CGMCC No.3265. The invention also provides the application of the acidithiobacillus ferrooxidans in metallurgy, particularly the acidithiobacillus ferrooxidans is applied to the leaching of molybdenum ore containing Fe2<+> or sulfides. In the invention, the acidithiobacillus ferrooxidans is added into the molybdenum ore pulp containing the Fe2<+>, the leaching temperature is between 28 DEG C and 32 DEG C, the leaching pH is between 1 and 3, and the Fe2<+> is oxidized into Fe3<+>; the acidithiobacillus ferrooxidans is also added into the molybdenum ore pulp containing Fe2<+> and sulfides, the leaching temperature is between 28 DEG C and 32 DEG C, the leaching pH is between 1 and 3, and the Fe2<+> and the sulfides are oxidized into Fe3<+> and sulfuric acid or sulfate respectively. The acidithiobacillus ferrooxidans provided by the invention has a stronger molybdenum resisting characteristic, can also grow well when the content of the molybdenum is 1.6 g / L, and can be widely applied to the leaching of molybdenum-bearing minerals.

Description

technical field [0001] The invention relates to an acidophilic chemoautotrophic bacterium used for the extraction and recovery of metals in metal ores, in particular to an acidophilic thiobacterium ferrooxidans and an application method thereof. Background technique [0002] Bacterial leaching technology is commonly used in the extraction and recovery of metals in low- and medium-grade metal ores. In conventional chemical leaching, a large amount of sulfuric acid and oxidants are artificially added for metal leaching. Due to the large consumption of sulfuric acid and oxidant, the process cost is also greatly improved. Bacterial leaching technology can use acidophilic autotrophic bacteria to overcome the above shortcomings. Acidophilic autotrophic bacteria mainly include ThiobaciLLus ferrooxidans (thiobacterium ferrooxidans), ThiobaciLLus thiooxidans (thiothiobacterium oxidans), FerrobaciLLus ferrooxidans (iron bacteria), LeptospiriLLum ferrooxidans (Leptospirulina ferrooxi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C22B34/34
CPCY02P10/20
Inventor 孟运生郑英周磊程浩刘辉师留印
Owner BEIJING RESEARCH INSTITUTE OF CHEMICAL ENGINEERING AND METALLURGY
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