Microbial agent capable of efficiently biologically converting form of cadmium in polluted soil

A microbial agent and biotransformation technology, applied in the restoration of contaminated soil, microorganisms, methods of using microorganisms, etc., can solve the problems of long time period and low leaching rate, and achieve difficult survival, short cultivation period and simple operation easy to control effect

Active Publication Date: 2017-07-14
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with the microbial agent of the present invention, they

Method used

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  • Microbial agent capable of efficiently biologically converting form of cadmium in polluted soil
  • Microbial agent capable of efficiently biologically converting form of cadmium in polluted soil

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: collection, cultivation and domestication of microbial bacterial agent

[0023] Medium preparation: 9K medium + 0.1% yeast extract + 0.1% glucose + 0.1% sulfur powder. 9K medium composition is as follows: distilled water 1000mL, (NH 4 ) 2 SO 4 3g / L, K 2 HPO 4 0.5g / L, KCl0.1g / L, Ca(NO 3 ) 2 0.01g / L, MgSO 4 ·7H 2 O0.5g / L. First, add 0.1% yeast extract to 9K medium to adjust the pH to 4.0, prepare 5% glucose solution at the same time, autoclave at 121°C for 20 minutes, and then weigh 0.1% sulfur powder for 20 minutes of ultraviolet sterilization before adding.

[0024] The bottom mud was collected from polluted farmland soil in the mining area of ​​Dapu Town, Hengyang, Hunan Province. The pollution period was as long as 40 years, and the cadmium content was as high as 100ppm. The five-point sampling method is used to collect 5-10cm deep soil samples polluted by high-concentration cadmium for a long time, mix them as the bottom mud for the preparatio...

Embodiment 2

[0027] Embodiment 2: the compounding of microbial bacterial agent of the present invention

[0028] According to the results of sequencing in Example 1, it is formed by mixing bacteria according to the following quantitative ratios: 25-35% Candida utilis (Candida utilis), 15-20% Schenck's Sporothrix sp., 15-35% 20% Acidithiobacillus ferrooxidans, 15-20% Sulfobacillus acidophilus, 5-15% Shewanella oneidensis; before mixing these bacteria in the cadmium-containing medium domesticated in.

[0029] The domestication process of the strain is as follows: set the concentration gradient of cadmium to 1mg / L, 5mg / L, 10mg / L, 15mg / L, 20mg / L, 25mg / L; the medium composition is: 9K medium +0.01~0.5% Yeast extract (m / v)+0.01%~0.5% glucose (m / v)+0.01~0.5%% sulfur powder (m / v); culture conditions: temperature 20~30°C, speed 150~200r / min, The initial pH was 4.0 for Candida utilis and Sporothrix schenckii and 2.0 for Thiobacillus ferrooxidans, Thiobacillus acidophilus and Ferro-dissimilarum. F...

Embodiment 3

[0032] Embodiment 3: microbial bacterial agent bioremoves cadmium in polluted soil

[0033] The cadmium-contaminated soil was collected from contaminated farmland soil in the mining area of ​​Dapu Town, Hengyang, Hunan Province, numbered A, B, and C. Air-dried the soil and ground it through a 100-mesh sieve, then weighed 20 g of the sample into clean small triangular flasks, packaged and sterilized. The bacterial agents used in the experiment include: the microbial bacterial agent a obtained in Example 2, the mine wastewater enrichment b of Example 1, the soil enrichment obtained by enriching the mine wastewater enrichment directly from cadmium-contaminated soil by the same method as that obtained in Example 1 Material c, the first generation enrichment d after the mixed culture of 5% mine wastewater enrichment and 5% bottom mud in Example 1.

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Abstract

The invention discloses a microbial agent capable of efficiently biologically converting a form of cadmium in polluted soil. The microbial agent can be utilized for efficiently converting cadmium difficult to utilize in the polluted soil into soluble cadmium and mainly contains the following components in percentage by weight: 25%-35% of Candida utilis 15%-20% of Sporotrichum sp., 15%-20% of Acidithiobacillus ferrooxidans, 15%-20% of Sulfobacillus acidophilus and 5%-15% of Shewanella oneidensis. By processing the soil by virtue of the microbial agent, the removal rate of cadmium is 82%-95%. Compared with an existing cadmium polluted soil converting and restoring technique, the microbial agent is capable of efficiently converting the form of cadmium, short in cycle, low in cost and easy to generalize.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of heavy metal pollution, and in particular relates to a microbial bacterial agent capable of efficiently biologically transforming the form of cadmium in polluted soil. Background technique [0002] With the development and utilization of mineral resources, the rapid development of industrial production and the widespread use of various chemical products, pesticides and fertilizers, pollutants containing heavy metals enter the environment through various channels, resulting in increasingly serious soil pollution. At present, the area of ​​cultivated land polluted by Cd, Hg, As, Cr, and Pb in my country is about 2000×10 4 hm 2 , the annual food loss due to heavy metal pollution is about 1000×10 4 t, contaminated grain up to 1200×10 4 t, the economic loss is at least 200×10 8 Yuan. [0003] Heavy metal pollutants cannot be chemically or biologically degraded, and are easy to accumu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C12N1/36B09C1/10C12R1/72C12R1/645C12R1/01
CPCB09C1/105C12N1/14C12N1/16C12N1/20C12N1/36
Inventor 刘学端柏连阳刘宏伟蒋慧丹邓燕
Owner CENT SOUTH UNIV
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