Acidophilic iron-oxidizing composite microbial agent and preparation method thereof

Abstract

The invention discloses an acidophilic iron-oxidizing composite microbial agent which comprises the following communities: a ferroplasma acidiphilum-containing strain, an acidithiobacillus ferrivorans-containing strain, an acidithiobacillus ferrooxidans-containing strain, a leptospirillum ferriphilum-containing strain and a sulfobacillus acidophilus-containing strain, wherein a preparation method of the composite microbial agent comprises the following steps: the aforementioned five strains are placed in a specific basic iron salt / nutrient-containing culture medium, and are subjected to the temperature gradient compounding culture, tolerance mixed-heavy-metal gradient pressure type domestication, temperature cycling gradient type domestication, ferrous oxidation activity improvement and tolerance high-iron domestication, so as to obtain the acidophilic iron-oxidizing composite microbial agent. According to the invention, the acidophilic iron-oxidizing composite microbial agent is simple in formulation, economical, adaptable, versatile and efficient, and can be used for the efficient extraction of heavy metals in electroplating sludge.

Description

technical field [0001] The invention belongs to the technical field of sludge harmless biological treatment in the field of environmental protection, and in particular relates to a microbial bacterial agent which can be used for the harmless treatment of electroplating sludge and its application. Background technique [0002] Electroplating sludge is the ultimate solid waste in the surface treatment industry. It has very complex components and high toxicity. The content is much higher than the grade of mineral development, and the potential value of reuse is high, so it has become a rare and cheap renewable non-ferrous metal resource. [0003] Traditional recycling of electroplating sludge mainly includes pyrometallurgy, chemical wet extraction or the combination of the two, but it has limitations such as high energy consumption, high cost, serious secondary pollution, and simple treatment objects. In recent years, the bioleaching (extraction) method has attracted more and ...

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Problems solved by technology

[0013] At present, although there have been a large number of reports on microorganisms that extract heavy metals from sludge by biological methods, the research results mainly focus on acidophilic iron / sulfur oxidizing microorganisms at room temperature. The acidophilic Thiobacillus ferrooxidans and acidophilic Thiobacillus thiooxidans, etc., thus the obtained strains and their community structure and function are relatively simple, which is not conducive to the cultivation and preparation of composite bacterial agents and sludge leaching treatment under high temperature and low temperature conditions , and it is not conducive to the efficient biological treatment of electroplating sludge from complex sources

In addition, the Chinese patent document CN 103451437 A discloses a method for recovering valuable metal sludge containing copper, cobalt and nickel, which is dominated by high-temperature archaea, which is assisted by bacteria. and high-efficiency biological treatment of electroplating sludge

In order to be applicable to different environmental pressure conditions all year round and to achieve efficient leaching of metals from sludge from different sources, the bioleaching process introduced in the existing patent literature must additionally add certain cultivation temperature control equipment and long-term sludge adaptive domestication process. This leads to long process flow, low efficiency and high cost, which is not conducive to the wide industrial application of biological extraction process

In view of the limitations of the microbial population structure and functional application of the current biological extraction sludge, it is of great significance to study a "universal and efficient" acidophilic microbial agent for extraction of heavy metals in electroplating sludge and its preparation method. There are few such reports

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