A kind of Lactobacillus rhamnosus and its application in the preparation of bacteriocin

A technology of Lactobacillus rhamnosus and bacteriocin, which is applied in the field of food microorganisms, can solve the problems of drug resistance of pathogenic bacteria and threaten human health, and achieve the effects of safe source, less toxic and side effects, and good thermal stability.

Active Publication Date: 2021-05-25
HENAN INST OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The growth of pathogenic bacteria can be inhibited by chemical drugs, but the abuse of antibiotics makes pathogenic bacteria resistant and seriously threatens human health, so natural products are getting more and more attention

Method used

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  • A kind of Lactobacillus rhamnosus and its application in the preparation of bacteriocin
  • A kind of Lactobacillus rhamnosus and its application in the preparation of bacteriocin
  • A kind of Lactobacillus rhamnosus and its application in the preparation of bacteriocin

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Embodiment 1

[0019] The Lactobacillus rhamnosus zrx01 is isolated and screened from kefir grains, and the frozen zrx01 strain is inoculated in the MRS medium, cultured at 200r / min, 37°C for 24h, and used as the primary seed solution. The primary seeds were inoculated into 100 mL MRS medium according to the inoculum amount of 2%, cultivated overnight as the secondary seed solution, and stored in a refrigerator at 4°C for later use. Streak the cultured secondary seed liquid zrx01 plate on the MRS plate, incubate at 37°C for 2-3 days, and observe the colony morphology. A single colony was picked and stained with Gram, and the morphology of the bacteria was observed under a microscope. The results showed that the bacterial colony of bacterial strain zrx01 was moist milky white drop shape, opaque, uniform in texture and neat in edge. Stained by Gram staining solution and observed under a microscope, the bacteria are short rod-shaped Gram-positive bacteria ( figure 1 ). Biochemical characteri...

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Abstract

The invention discloses a Lactobacillus rhamnosus and its application in the preparation of bacteriocins. The preserved taxonomic name of the Lactobacillus rhamnosus is: Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) zrx01; deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms; date of preservation: December 22, 2017; preservation number is CGMCC No.15076. The bacteriocin produced by Lactobacillus rhamnosus zrx01 screened by the present invention can inhibit a variety of harmful bacteria, especially for Gram-positive bacteria Staphylococcus aureus, Listeria monocytogenes, Bacillus anthracis, Gram-negative bacteria large intestine Bacillus, Campylobacter jejuni, and Alicyclobacillus acidterreus have strong inhibitory effects, but they have no inhibitory effect on probiotics such as Lactobacillus plantarum and Lactobacillus acidophilus. Same non-inhibitory.

Description

technical field [0001] The invention belongs to the technical field of food microorganisms, in particular to a kind of Lactobacillus rhamnosus and its application in the preparation of bacteriocins. Background technique [0002] Food safety is an important aspect of people's choice of food, and diseases caused by foodborne microorganisms are widespread in both developed and developing countries, posing a serious threat to public health. After years of research, food pathogenic microorganisms related to health hazards have been identified, such as Salmonella enteritidis and Escherichia coli, which cause 75% of foodborne diseases. The growth of pathogenic bacteria can be inhibited by chemical drugs, but the abuse of antibiotics has caused pathogenic bacteria to develop drug resistance, which seriously threatens human health, so natural products have received more and more attention. Bacteriocins are peptides or proteins with antibacterial activity produced by lactic acid bact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/225
CPCC07K14/335C12N1/205C12R2001/225
Inventor 冉军舰朱明明焦凌霞梁新红赵瑞香卢艳清
Owner HENAN INST OF SCI & TECH
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