Method for synchronously controlling alicyclobacillus in water and odor metabolism pollutant of alicyclobacillus

A bacillus, synchronous control technology, applied in the direction of water pollutants, water/sewage treatment, chemical instruments and methods, etc., can solve the problems of cost increase, stimulation of spores, poor stability of interception effect, etc., to reduce the dosage and improve the elimination Bacteria effect, low energy consumption effect

Inactive Publication Date: 2021-08-13
中国人民解放军疾病预防控制中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical disinfectants are commonly used in the process of fruit and vegetable processing and cleaning to kill spoilage microorganisms, but disinfection by-products harmful to human health will be produced during the disinfection process, and for Alicyclobacillus with strong stress resistance, inactivation The effect is not ideal
The commonly used sterilization methods in the food processing and production process are: 1. Membrane filtration, which requires frequent replacement of the filter membrane, resulting in increased costs and poor retention stability
2. High-temperature sterilization, because Alicyclobacillus is a heat-resistant bacterium, general high-temperature sterilization cannot kill it, and ultra-high temperature will affect the flavor of fruit juice
3. Pasteurization, conventional pasteurization is difficult to completely inactivate it, but may stimulate spores to germinate
Existing ultraviolet sterilization is generally irradiated by single-wavelength low-pressure mercury lamps (254nm). Due to the poor penetration of ultraviolet rays, it is usually used for the treatment of food factory workshops, equipment, surfaces of packaging materials, and processing water.

Method used

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  • Method for synchronously controlling alicyclobacillus in water and odor metabolism pollutant of alicyclobacillus
  • Method for synchronously controlling alicyclobacillus in water and odor metabolism pollutant of alicyclobacillus
  • Method for synchronously controlling alicyclobacillus in water and odor metabolism pollutant of alicyclobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Separate low-pressure ultraviolet ray irradiation sterilization

[0021] Add the spore suspension of Alicyclobacillus in a glass petri dish, utilize a 30W low-pressure mercury lamp to emit low-pressure ultraviolet light (254nm), and use a UV-B type ultraviolet radiation meter to measure the light intensity, and calculate according to the set irradiation dose corresponding exposure time. After reaching the estimated irradiation time, immediately add 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples and count them. Draw a graph of the relationship between the inactivation rate and the irradiation dose, such as figure 1 shown in the curve.

Embodiment 2

[0022] Embodiment 2: Sterilization by medium-pressure ultraviolet irradiation alone

[0023] Add the spore suspension of Alicyclobacillus into a glass petri dish, use a 3kW medium-pressure mercury lamp to emit medium-pressure ultraviolet rays, measure the light intensity with an ILT1700 ultraviolet radiation meter, and calculate the corresponding irradiation time according to the set irradiation dose . After reaching the estimated irradiation time, immediately add 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples and count them. Draw a graph of the relationship between the inactivation rate and the irradiation dose, such as figure 2 shown in the curve.

Embodiment 3

[0024] Embodiment 3: Potassium persulfate processing alone

[0025] Add the spore suspension of Alicyclobacillus to the brown bottle, shake and mix immediately after adding different concentrations of potassium persulfate solution. After reacting in the dark at room temperature for a certain period of time, immediately add a 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples for counting. Draw a graph of the relationship between the inactivation rate and the treatment time, such as image 3 shown in the curve.

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Abstract

The invention provides a method for synchronously controlling alicyclobacillus in water and guaiacol as an odor metabolism pollutant of the alicyclobacillus. Medium-pressure ultraviolet rays are adopted to sterilize and irradiate alicyclobacillus in water, so that the alicyclobacillus is prevented from entering a subsequent production link to cause cross contamination among production equipment from the source, and therefore, the quality of terminal products in the fruit and vegetable industry is ensured. Medium-pressure ultraviolet rays and potassium persulfate are combined for use, so that the irradiation dose of the medium-pressure ultraviolet rays can be properly reduced on the premise of ensuring a relatively good sterilization effect, a large amount of harmful disinfection byproducts cannot be generated in a treatment process, the method is safer to use in a food production and processing process, and particularly, the method has a relatively good application prospect in the aspects of processing and cleaning water for fruits and vegetables and disinfecting water for other foods.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for simultaneously controlling Alicyclobacillus and its odor-taste metabolic pollutant guaiacol in fruit and vegetable processing and cleaning water by using medium-pressure ultraviolet light combined with potassium persulfate. Background technique [0002] Juice spoilage incidents caused by Alicyclobacillus have occurred successively in many countries and regions in recent years, bringing huge economic losses to juice production enterprises. The bacteria has become one of the most concerned pollutants in the juice industry. [0003] Alicyclobacillus (Alicyclobacillus spp.) is acidophilic, heat-resistant, spore-forming, and stress-resistant. Guaiacol, a pollutant of its odor and taste metabolism, will affect the flavor of fruit juice at very low concentrations. The spores of the bacteria can contaminate semi-finished products or production materials in fruit juic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/32C02F1/72C02F101/34C02F103/32
CPCC02F1/32C02F1/722C02F1/72C02F2101/345C02F2303/02C02F2305/02C02F2103/32
Inventor 张灿白淼张明露张传福徐绍峰
Owner 中国人民解放军疾病预防控制中心
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